Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry

This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quali...

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Detalles Bibliográficos
Autores principales: Jennings, Emma K., Lecky, David A.J., Ono, Masahiro, Bending, David
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821036/
https://www.ncbi.nlm.nih.gov/pubmed/33532733
http://dx.doi.org/10.1016/j.xpro.2020.100284
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author Jennings, Emma K.
Lecky, David A.J.
Ono, Masahiro
Bending, David
author_facet Jennings, Emma K.
Lecky, David A.J.
Ono, Masahiro
Bending, David
author_sort Jennings, Emma K.
collection PubMed
description This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining. For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020.
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spelling pubmed-78210362021-02-01 Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry Jennings, Emma K. Lecky, David A.J. Ono, Masahiro Bending, David STAR Protoc Protocol This protocol uses Nr4a1-GFP Nr4a3-Tocky mice to study T cell receptor (TCR) signaling using flow cytometry. It identifies the optimal mouse transgenic status and fluorochromes compatible with the dual reporter. This protocol has applications in TCR signaling, and we outline how to obtain high-quality datasets. It is not compatible with cellular fixation, and cells should be analyzed immediately after staining. For complete details on the use and execution of this protocol, please refer to Jennings et al., 2020. Elsevier 2021-01-21 /pmc/articles/PMC7821036/ /pubmed/33532733 http://dx.doi.org/10.1016/j.xpro.2020.100284 Text en © 2020 The Author(s) http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Protocol
Jennings, Emma K.
Lecky, David A.J.
Ono, Masahiro
Bending, David
Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry
title Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry
title_full Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry
title_fullStr Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry
title_full_unstemmed Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry
title_short Application of dual Nr4a1-GFP Nr4a3-Tocky reporter mice to study T cell receptor signaling by flow cytometry
title_sort application of dual nr4a1-gfp nr4a3-tocky reporter mice to study t cell receptor signaling by flow cytometry
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821036/
https://www.ncbi.nlm.nih.gov/pubmed/33532733
http://dx.doi.org/10.1016/j.xpro.2020.100284
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