Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells

Cytosolic Ca(2+) levels are maintained at low nanomolar concentrations, and disruption of Ca(2+) homeostasis is associated with cell/tissue damage. Thus, methods have been developed to accurately assess cellular Ca(2+) levels, each with intrinsic advantages and disadvantages. Here, we present in det...

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Detalles Bibliográficos
Autores principales: Rodrigues, Tiago, Ferraz, Letícia Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821037/
https://www.ncbi.nlm.nih.gov/pubmed/33532731
http://dx.doi.org/10.1016/j.xpro.2020.100282
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author Rodrigues, Tiago
Ferraz, Letícia Silva
author_facet Rodrigues, Tiago
Ferraz, Letícia Silva
author_sort Rodrigues, Tiago
collection PubMed
description Cytosolic Ca(2+) levels are maintained at low nanomolar concentrations, and disruption of Ca(2+) homeostasis is associated with cell/tissue damage. Thus, methods have been developed to accurately assess cellular Ca(2+) levels, each with intrinsic advantages and disadvantages. Here, we present in detail a ratiometric fluorometric method for cytosolic Ca(2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging. For complete details on the use and execution of this protocol, please refer to Esteves et al. (2020).
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spelling pubmed-78210372021-02-01 Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells Rodrigues, Tiago Ferraz, Letícia Silva STAR Protoc Protocol Cytosolic Ca(2+) levels are maintained at low nanomolar concentrations, and disruption of Ca(2+) homeostasis is associated with cell/tissue damage. Thus, methods have been developed to accurately assess cellular Ca(2+) levels, each with intrinsic advantages and disadvantages. Here, we present in detail a ratiometric fluorometric method for cytosolic Ca(2+) measurement in cultured melanoma cells using Fura 2-AM cell loading and fluorescence microscopy imaging. For complete details on the use and execution of this protocol, please refer to Esteves et al. (2020). Elsevier 2021-01-21 /pmc/articles/PMC7821037/ /pubmed/33532731 http://dx.doi.org/10.1016/j.xpro.2020.100282 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Rodrigues, Tiago
Ferraz, Letícia Silva
Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
title Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
title_full Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
title_fullStr Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
title_full_unstemmed Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
title_short Cytosolic Ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
title_sort cytosolic ca(2+) measurements by ratiometric fluorescence microscopy in melanoma cells
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821037/
https://www.ncbi.nlm.nih.gov/pubmed/33532731
http://dx.doi.org/10.1016/j.xpro.2020.100282
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AT ferrazleticiasilva cytosolicca2measurementsbyratiometricfluorescencemicroscopyinmelanomacells

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