High-content imaging-based pooled CRISPR screens in mammalian cells

CRISPR (clustered regularly interspaced short palindromic repeats)-based gene inactivation provides a powerful means for linking genes to particular cellular phenotypes. CRISPR-based screening typically uses large genomic pools of single guide RNAs (sgRNAs). However, this approach is limited to phen...

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Detalles Bibliográficos
Autores principales: Yan, Xiaowei, Stuurman, Nico, Ribeiro, Susana A., Tanenbaum, Marvin E., Horlbeck, Max A., Liem, Christina R., Jost, Marco, Weissman, Jonathan S., Vale, Ronald D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2021
Materias:
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821101/
https://www.ncbi.nlm.nih.gov/pubmed/33465779
http://dx.doi.org/10.1083/jcb.202008158

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