Cargando…
Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat
KEY POINTS: Rhythmic processes in living organisms are controlled by biological clocks. The orexinergic system of the lateral hypothalamus carries circadian information to provide arousal for the brain during the active phase. Here, we show that orexins exert an excitatory action in three parts of t...
Autores principales: | , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821336/ https://www.ncbi.nlm.nih.gov/pubmed/32997815 http://dx.doi.org/10.1113/JP280275 |
_version_ | 1783639402161897472 |
---|---|
author | Chrobok, Lukasz Jeczmien‐Lazur, Jagoda Stanislawa Pradel, Kamil Klich, Jasmin Daniela Bubka, Monika Wojcik, Michal Kepczynski, Mariusz Lewandowski, Marian Henryk |
author_facet | Chrobok, Lukasz Jeczmien‐Lazur, Jagoda Stanislawa Pradel, Kamil Klich, Jasmin Daniela Bubka, Monika Wojcik, Michal Kepczynski, Mariusz Lewandowski, Marian Henryk |
author_sort | Chrobok, Lukasz |
collection | PubMed |
description | KEY POINTS: Rhythmic processes in living organisms are controlled by biological clocks. The orexinergic system of the lateral hypothalamus carries circadian information to provide arousal for the brain during the active phase. Here, we show that orexins exert an excitatory action in three parts of the lateral geniculate nucleus (LGN), in particular upon directly retinorecipient neurons in the non‐image forming visual structures. We provide evidence for the high nocturnal levels of orexins with stable circadian expression of predominant orexin receptor 2 in the LGN. Our data additionally establish the convergence of orexinergic and pituitary adenylate cyclase (PAC)‐activating peptide/PAC1 receptor systems (used by melanopsin‐expressing retinal ganglion cells), which directly regulates responses to the retinal input. These results help us better understand circadian orexinergic control over the non‐image forming subcortical visual system, forming the animal's preparedness for the behaviourally active night. ABSTRACT: The orexinergic system of the lateral hypothalamus is tightly interlinked with the master circadian clock and displays daily variation in activity to provide arousal‐related excitation for the plethora of brain structures in a circadian manner. Here, using a combination of electrophysiological, optogenetic, histological, molecular and neuronal tracing methods, we explore a particular link between orexinergic and visual systems in rat. The results of the present study demonstrate that orexinergic fibre density at the area of subcortical visual system exerts a clear day to night variability, reaching a maximum at behaviourally active night. We also show pronounced electrophysiological activations of neurons in the lateral geniculate nucleus by orexin A through 24 h, via identified distinct orexin receptors, with the ventrolateral geniculate displaying a daily cycle of responsiveness. In addition, for the first time, we provide a direct evidence for orexins to act on retinorecipient neurons with a high convergence of orexinergic and putatively retinal pituitary adenylate cyclase (PAC)‐activating peptide/PAC1 receptor systems. Altogether, the present study ties orexins to non‐image forming visual structures with implications for circadian orexinergic modulation of neurons, which process information on ambient light levels. |
format | Online Article Text |
id | pubmed-7821336 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-78213362021-01-29 Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat Chrobok, Lukasz Jeczmien‐Lazur, Jagoda Stanislawa Pradel, Kamil Klich, Jasmin Daniela Bubka, Monika Wojcik, Michal Kepczynski, Mariusz Lewandowski, Marian Henryk J Physiol Neuroscience KEY POINTS: Rhythmic processes in living organisms are controlled by biological clocks. The orexinergic system of the lateral hypothalamus carries circadian information to provide arousal for the brain during the active phase. Here, we show that orexins exert an excitatory action in three parts of the lateral geniculate nucleus (LGN), in particular upon directly retinorecipient neurons in the non‐image forming visual structures. We provide evidence for the high nocturnal levels of orexins with stable circadian expression of predominant orexin receptor 2 in the LGN. Our data additionally establish the convergence of orexinergic and pituitary adenylate cyclase (PAC)‐activating peptide/PAC1 receptor systems (used by melanopsin‐expressing retinal ganglion cells), which directly regulates responses to the retinal input. These results help us better understand circadian orexinergic control over the non‐image forming subcortical visual system, forming the animal's preparedness for the behaviourally active night. ABSTRACT: The orexinergic system of the lateral hypothalamus is tightly interlinked with the master circadian clock and displays daily variation in activity to provide arousal‐related excitation for the plethora of brain structures in a circadian manner. Here, using a combination of electrophysiological, optogenetic, histological, molecular and neuronal tracing methods, we explore a particular link between orexinergic and visual systems in rat. The results of the present study demonstrate that orexinergic fibre density at the area of subcortical visual system exerts a clear day to night variability, reaching a maximum at behaviourally active night. We also show pronounced electrophysiological activations of neurons in the lateral geniculate nucleus by orexin A through 24 h, via identified distinct orexin receptors, with the ventrolateral geniculate displaying a daily cycle of responsiveness. In addition, for the first time, we provide a direct evidence for orexins to act on retinorecipient neurons with a high convergence of orexinergic and putatively retinal pituitary adenylate cyclase (PAC)‐activating peptide/PAC1 receptor systems. Altogether, the present study ties orexins to non‐image forming visual structures with implications for circadian orexinergic modulation of neurons, which process information on ambient light levels. John Wiley and Sons Inc. 2020-10-14 2021-01-01 /pmc/articles/PMC7821336/ /pubmed/32997815 http://dx.doi.org/10.1113/JP280275 Text en © 2020 The Authors. The Journal of Physiology published by John Wiley & Sons Ltd on behalf of The Physiological Society This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Neuroscience Chrobok, Lukasz Jeczmien‐Lazur, Jagoda Stanislawa Pradel, Kamil Klich, Jasmin Daniela Bubka, Monika Wojcik, Michal Kepczynski, Mariusz Lewandowski, Marian Henryk Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
title | Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
title_full | Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
title_fullStr | Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
title_full_unstemmed | Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
title_short | Circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
title_sort | circadian actions of orexins on the retinorecipient lateral geniculate complex in rat |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821336/ https://www.ncbi.nlm.nih.gov/pubmed/32997815 http://dx.doi.org/10.1113/JP280275 |
work_keys_str_mv | AT chroboklukasz circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT jeczmienlazurjagodastanislawa circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT pradelkamil circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT klichjasmindaniela circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT bubkamonika circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT wojcikmichal circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT kepczynskimariusz circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat AT lewandowskimarianhenryk circadianactionsoforexinsontheretinorecipientlateralgeniculatecomplexinrat |