Modifications to the classical rat aortic ring model to allow vascular degeneration studies

The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days...

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Autores principales: Blot, Guillaume, Sartoris, Thérèse-Marie, Sennlaub, Florian, Guillonneau, Xavier
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2021
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821348/
https://www.ncbi.nlm.nih.gov/pubmed/33532730
http://dx.doi.org/10.1016/j.xpro.2020.100281
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author Blot, Guillaume
Sartoris, Thérèse-Marie
Sennlaub, Florian
Guillonneau, Xavier
author_facet Blot, Guillaume
Sartoris, Thérèse-Marie
Sennlaub, Florian
Guillonneau, Xavier
author_sort Blot, Guillaume
collection PubMed
description The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days.
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spelling pubmed-78213482021-02-01 Modifications to the classical rat aortic ring model to allow vascular degeneration studies Blot, Guillaume Sartoris, Thérèse-Marie Sennlaub, Florian Guillonneau, Xavier STAR Protoc Protocol The classical aortic ring model is well suited for deciphering pro-angiogenic processes. Here, we propose simple modifications of the standard protocol to study various anti-angiogenic processes from growth arrest to capillary degeneration. Aortic rings are cultured under basal conditions for 6 days to allow physiological vessel sprouting and then split into treatment groups to follow capillary growth or degeneration for an additional 2 days. Elsevier 2021-01-21 /pmc/articles/PMC7821348/ /pubmed/33532730 http://dx.doi.org/10.1016/j.xpro.2020.100281 Text en © 2021 The Authors http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Blot, Guillaume
Sartoris, Thérèse-Marie
Sennlaub, Florian
Guillonneau, Xavier
Modifications to the classical rat aortic ring model to allow vascular degeneration studies
title Modifications to the classical rat aortic ring model to allow vascular degeneration studies
title_full Modifications to the classical rat aortic ring model to allow vascular degeneration studies
title_fullStr Modifications to the classical rat aortic ring model to allow vascular degeneration studies
title_full_unstemmed Modifications to the classical rat aortic ring model to allow vascular degeneration studies
title_short Modifications to the classical rat aortic ring model to allow vascular degeneration studies
title_sort modifications to the classical rat aortic ring model to allow vascular degeneration studies
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821348/
https://www.ncbi.nlm.nih.gov/pubmed/33532730
http://dx.doi.org/10.1016/j.xpro.2020.100281
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