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Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds

BACKGROUND: Lawsonia intracellularis is causing diarrhea, poor growth and sudden death in pigs. It can be found in most pig populations leading to large economic losses worldwide. Many potential risk factors for the occurrence of disease or seropositivity have been described. The current study there...

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Autores principales: Arnold, Mirjam, Crienen, Annelies, Swam, Hanny, Berg, Stephan v., Jolie, Rika, Nathues, Heiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821494/
https://www.ncbi.nlm.nih.gov/pubmed/33482877
http://dx.doi.org/10.1186/s40813-021-00192-4
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author Arnold, Mirjam
Crienen, Annelies
Swam, Hanny
Berg, Stephan v.
Jolie, Rika
Nathues, Heiko
author_facet Arnold, Mirjam
Crienen, Annelies
Swam, Hanny
Berg, Stephan v.
Jolie, Rika
Nathues, Heiko
author_sort Arnold, Mirjam
collection PubMed
description BACKGROUND: Lawsonia intracellularis is causing diarrhea, poor growth and sudden death in pigs. It can be found in most pig populations leading to large economic losses worldwide. Many potential risk factors for the occurrence of disease or seropositivity have been described. The current study therefore focused on herd characteristics in European countries associated with direct detection of the pathogen determined by quantitative polymerase chain reaction. RESULTS: A median number of less than 30 nursery pigs per pen was correlated to less positive nursery pigs (p < 0.01) and generally less samples positive per herd (p < 0.05) as well as a lower median of genome equivalents determined per herd (p < 0.05). Routine use of zinc oxide at/ around weaning, which was mentioned by 41.0% of all farmers, was correlated to higher number of positive nursery pigs (p < 0.01) as well as higher median genome equivalents determined per herd (p < 0.05). Slatted flooring of more than 78.0% of the surface in nursery units was correlated to lower number of positive animals (p < 0.05) and a lower median of genome equivalents per herd (p < 0.05). A weight of more than 7.8 kg at weaning was correlated to a higher number of positive growing pigs (p < 0.05) as well as general higher number of positive samples/ herd (p < 0.01). CONCLUSIONS: Weaning and subsequent accommodation of nursery pigs seem to be of particular importance in prevention of infection with Lawsonia intracellularis and the spread of the pathogen within the herd. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40813-021-00192-4.
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spelling pubmed-78214942021-01-22 Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds Arnold, Mirjam Crienen, Annelies Swam, Hanny Berg, Stephan v. Jolie, Rika Nathues, Heiko Porcine Health Manag Research BACKGROUND: Lawsonia intracellularis is causing diarrhea, poor growth and sudden death in pigs. It can be found in most pig populations leading to large economic losses worldwide. Many potential risk factors for the occurrence of disease or seropositivity have been described. The current study therefore focused on herd characteristics in European countries associated with direct detection of the pathogen determined by quantitative polymerase chain reaction. RESULTS: A median number of less than 30 nursery pigs per pen was correlated to less positive nursery pigs (p < 0.01) and generally less samples positive per herd (p < 0.05) as well as a lower median of genome equivalents determined per herd (p < 0.05). Routine use of zinc oxide at/ around weaning, which was mentioned by 41.0% of all farmers, was correlated to higher number of positive nursery pigs (p < 0.01) as well as higher median genome equivalents determined per herd (p < 0.05). Slatted flooring of more than 78.0% of the surface in nursery units was correlated to lower number of positive animals (p < 0.05) and a lower median of genome equivalents per herd (p < 0.05). A weight of more than 7.8 kg at weaning was correlated to a higher number of positive growing pigs (p < 0.05) as well as general higher number of positive samples/ herd (p < 0.01). CONCLUSIONS: Weaning and subsequent accommodation of nursery pigs seem to be of particular importance in prevention of infection with Lawsonia intracellularis and the spread of the pathogen within the herd. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s40813-021-00192-4. BioMed Central 2021-01-22 /pmc/articles/PMC7821494/ /pubmed/33482877 http://dx.doi.org/10.1186/s40813-021-00192-4 Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Arnold, Mirjam
Crienen, Annelies
Swam, Hanny
Berg, Stephan v.
Jolie, Rika
Nathues, Heiko
Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds
title Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds
title_full Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds
title_fullStr Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds
title_full_unstemmed Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds
title_short Correlation of Lawsonia intracellularis positivity in quantitative PCR and herd factors in European pig herds
title_sort correlation of lawsonia intracellularis positivity in quantitative pcr and herd factors in european pig herds
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821494/
https://www.ncbi.nlm.nih.gov/pubmed/33482877
http://dx.doi.org/10.1186/s40813-021-00192-4
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