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miR-335-5p suppresses gastric cancer progression by targeting MAPK10

BACKGROUND: Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlyi...

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Autores principales: Gao, Yi, Wang, Yanfeng, Wang, Xiaofei, Zhao, Changan, Wang, Fenghui, Du, Juan, Zhang, Huahua, Shi, Haiyan, Feng, Yun, Li, Dan, Yan, Jing, Yao, Yan, Hu, Weihong, Ding, Ruxin, Zhang, Mengjie, Wang, Lumin, Huang, Chen, Zhang, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821696/
https://www.ncbi.nlm.nih.gov/pubmed/33482821
http://dx.doi.org/10.1186/s12935-020-01684-z
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author Gao, Yi
Wang, Yanfeng
Wang, Xiaofei
Zhao, Changan
Wang, Fenghui
Du, Juan
Zhang, Huahua
Shi, Haiyan
Feng, Yun
Li, Dan
Yan, Jing
Yao, Yan
Hu, Weihong
Ding, Ruxin
Zhang, Mengjie
Wang, Lumin
Huang, Chen
Zhang, Jing
author_facet Gao, Yi
Wang, Yanfeng
Wang, Xiaofei
Zhao, Changan
Wang, Fenghui
Du, Juan
Zhang, Huahua
Shi, Haiyan
Feng, Yun
Li, Dan
Yan, Jing
Yao, Yan
Hu, Weihong
Ding, Ruxin
Zhang, Mengjie
Wang, Lumin
Huang, Chen
Zhang, Jing
author_sort Gao, Yi
collection PubMed
description BACKGROUND: Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated. METHODS: Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting. RESULTS: The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p. CONCLUSIONS: These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression.
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spelling pubmed-78216962021-01-25 miR-335-5p suppresses gastric cancer progression by targeting MAPK10 Gao, Yi Wang, Yanfeng Wang, Xiaofei Zhao, Changan Wang, Fenghui Du, Juan Zhang, Huahua Shi, Haiyan Feng, Yun Li, Dan Yan, Jing Yao, Yan Hu, Weihong Ding, Ruxin Zhang, Mengjie Wang, Lumin Huang, Chen Zhang, Jing Cancer Cell Int Primary Research BACKGROUND: Recent studies have established the roles of microRNAs (miRNAs) in cancer progression. The aberrant expression of miR-335-5p has been reported in many cancers, including gastric cancer (GC). In this study, the precise roles of miR-335-5p in GC as well as the molecular mechanisms underlying its effects, including the role of its target MAPK10, were evaluated. METHODS: Quantitative real-time PCR was used to evaluate miR-335-5p levels in GC cell lines and tissues. MTT and colony formation assays were used to detect cell proliferation, and Transwell and wound-healing assays were used to evaluate the invasion and migration of GC cells. The correlation between levels of miR-335-5p and the cell cycle-related target gene mitogen-activated protein kinase 10 (MAPK10) in GC was analyzed. In addition, the candidate target was evaluated by a luciferase reporter assay, qRT-PCR, and western blotting. RESULTS: The levels of miR-335-5p were downregulated in GC tissues and cell lines. Furthermore, miR-335-5p inhibited the proliferation and migration of GC cells and induced apoptosis. Additionally, miR-335-5p arrested the cell cycle at the G1/S phase in GC cells in vitro. Levels of miR-335-5p and the cell cycle-related target gene MAPK10 in GC were correlated, and MAPK10 was directly targeted by miR-335-5p. CONCLUSIONS: These data suggest that miR-335-5p is a tumor suppressor and acts via MAPK10 to inhibit GC progression. BioMed Central 2021-01-22 /pmc/articles/PMC7821696/ /pubmed/33482821 http://dx.doi.org/10.1186/s12935-020-01684-z Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Primary Research
Gao, Yi
Wang, Yanfeng
Wang, Xiaofei
Zhao, Changan
Wang, Fenghui
Du, Juan
Zhang, Huahua
Shi, Haiyan
Feng, Yun
Li, Dan
Yan, Jing
Yao, Yan
Hu, Weihong
Ding, Ruxin
Zhang, Mengjie
Wang, Lumin
Huang, Chen
Zhang, Jing
miR-335-5p suppresses gastric cancer progression by targeting MAPK10
title miR-335-5p suppresses gastric cancer progression by targeting MAPK10
title_full miR-335-5p suppresses gastric cancer progression by targeting MAPK10
title_fullStr miR-335-5p suppresses gastric cancer progression by targeting MAPK10
title_full_unstemmed miR-335-5p suppresses gastric cancer progression by targeting MAPK10
title_short miR-335-5p suppresses gastric cancer progression by targeting MAPK10
title_sort mir-335-5p suppresses gastric cancer progression by targeting mapk10
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821696/
https://www.ncbi.nlm.nih.gov/pubmed/33482821
http://dx.doi.org/10.1186/s12935-020-01684-z
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