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RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report

Retinoic acid inducible gene I (RIG-I) is associated to the DExD/H box RNA helicases. It is a pattern recognition receptor (PRR), playing a crucial role in the system and is a germ line encoded host sensor to perceive pathogen-associated molecular patterns (PAMPs). So far, reports are available for...

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Autores principales: Banerjee, Samiddha, Pal, Aruna, Pal, Abantika, Mandal, Subhas Chandra, Chatterjee, Paresh Nath, Chatterjee, Jayanta Kumar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821740/
https://www.ncbi.nlm.nih.gov/pubmed/33488570
http://dx.doi.org/10.3389/fimmu.2020.534705
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author Banerjee, Samiddha
Pal, Aruna
Pal, Abantika
Mandal, Subhas Chandra
Chatterjee, Paresh Nath
Chatterjee, Jayanta Kumar
author_facet Banerjee, Samiddha
Pal, Aruna
Pal, Abantika
Mandal, Subhas Chandra
Chatterjee, Paresh Nath
Chatterjee, Jayanta Kumar
author_sort Banerjee, Samiddha
collection PubMed
description Retinoic acid inducible gene I (RIG-I) is associated to the DExD/H box RNA helicases. It is a pattern recognition receptor (PRR), playing a crucial role in the system and is a germ line encoded host sensor to perceive pathogen-associated molecular patterns (PAMPs). So far, reports are available for the role of RIG-I in antiviral immunity. This is the first report in which we have documented the role of RIG-I in parasitic immunity. Haemonchus contortus is a deadly parasite affecting the sheep industry, which has a tremendous economic importance, and the parasite is reported to be prevalent in the hot and humid agroclimatic region. We characterize the RIG-I gene in sheep (Ovis aries) and identify the important domains or binding sites with Haemonchus contortus through in silico studies. Differential mRNA expression analysis reveals upregulation of the RIG-I gene in the abomasum of infected sheep compared with that of healthy sheep, further confirming the findings. Thus, it is evident that, in infected sheep, expression of RIG-I is triggered for binding to more pathogens (Haemonchus contortus). Genetically similar studies with humans and other livestock species were conducted to reveal that sheep may be efficiently using a model organism for studying the role of RIG-I in antiparasitic immunity in humans.
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spelling pubmed-78217402021-01-23 RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report Banerjee, Samiddha Pal, Aruna Pal, Abantika Mandal, Subhas Chandra Chatterjee, Paresh Nath Chatterjee, Jayanta Kumar Front Immunol Immunology Retinoic acid inducible gene I (RIG-I) is associated to the DExD/H box RNA helicases. It is a pattern recognition receptor (PRR), playing a crucial role in the system and is a germ line encoded host sensor to perceive pathogen-associated molecular patterns (PAMPs). So far, reports are available for the role of RIG-I in antiviral immunity. This is the first report in which we have documented the role of RIG-I in parasitic immunity. Haemonchus contortus is a deadly parasite affecting the sheep industry, which has a tremendous economic importance, and the parasite is reported to be prevalent in the hot and humid agroclimatic region. We characterize the RIG-I gene in sheep (Ovis aries) and identify the important domains or binding sites with Haemonchus contortus through in silico studies. Differential mRNA expression analysis reveals upregulation of the RIG-I gene in the abomasum of infected sheep compared with that of healthy sheep, further confirming the findings. Thus, it is evident that, in infected sheep, expression of RIG-I is triggered for binding to more pathogens (Haemonchus contortus). Genetically similar studies with humans and other livestock species were conducted to reveal that sheep may be efficiently using a model organism for studying the role of RIG-I in antiparasitic immunity in humans. Frontiers Media S.A. 2021-01-08 /pmc/articles/PMC7821740/ /pubmed/33488570 http://dx.doi.org/10.3389/fimmu.2020.534705 Text en Copyright © 2021 Banerjee, Pal, Pal, Mandal, Chatterjee and Chatterjee http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Banerjee, Samiddha
Pal, Aruna
Pal, Abantika
Mandal, Subhas Chandra
Chatterjee, Paresh Nath
Chatterjee, Jayanta Kumar
RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report
title RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report
title_full RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report
title_fullStr RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report
title_full_unstemmed RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report
title_short RIG-I Has a Role in Immunity Against Haemonchus contortus, a Gastrointestinal Parasite in Ovis aries: A Novel Report
title_sort rig-i has a role in immunity against haemonchus contortus, a gastrointestinal parasite in ovis aries: a novel report
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7821740/
https://www.ncbi.nlm.nih.gov/pubmed/33488570
http://dx.doi.org/10.3389/fimmu.2020.534705
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