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Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822047/ https://www.ncbi.nlm.nih.gov/pubmed/33375072 http://dx.doi.org/10.3390/cells10010004 |
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author | Kretschmer, Rafael Gunski, Ricardo José Garnero, Analía del Valle de Freitas, Thales Renato Ochotorena Toma, Gustavo Akira Cioffi, Marcelo de Bello de Oliveira, Edivaldo Herculano Corrêa O’Connor, Rebecca E. Griffin, Darren K. |
author_facet | Kretschmer, Rafael Gunski, Ricardo José Garnero, Analía del Valle de Freitas, Thales Renato Ochotorena Toma, Gustavo Akira Cioffi, Marcelo de Bello de Oliveira, Edivaldo Herculano Corrêa O’Connor, Rebecca E. Griffin, Darren K. |
author_sort | Kretschmer, Rafael |
collection | PubMed |
description | Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined conventional and molecular cytogenetic approaches to investigate chromosome homologies between chicken and the smooth-billed ani (Crotophaga ani). Our results demonstrate extensive chromosome reorganization in C. ani, with interchromosomal rearrangements involving macro and microchromosomes. Intrachromosomal rearrangements were observed in some macrochromosomes, including the Z chromosome. The most evolutionary notable finding was a Robertsonian translocation between the microchromosome 17 and the Z chromosome, a rare event in birds. Additionally, the simple short repeats (SSRs) tested here were preferentially accumulated in the microchromosomes and in the Z and W chromosomes, showing no relationship with the constitutive heterochromatin regions, except in the W chromosome. Taken together, our results suggest that the avian sex chromosome is more complex than previously postulated and revealed the role of microchromosomes in the avian sex chromosome evolution, especially cuckoos. |
format | Online Article Text |
id | pubmed-7822047 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78220472021-01-23 Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation Kretschmer, Rafael Gunski, Ricardo José Garnero, Analía del Valle de Freitas, Thales Renato Ochotorena Toma, Gustavo Akira Cioffi, Marcelo de Bello de Oliveira, Edivaldo Herculano Corrêa O’Connor, Rebecca E. Griffin, Darren K. Cells Article Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined conventional and molecular cytogenetic approaches to investigate chromosome homologies between chicken and the smooth-billed ani (Crotophaga ani). Our results demonstrate extensive chromosome reorganization in C. ani, with interchromosomal rearrangements involving macro and microchromosomes. Intrachromosomal rearrangements were observed in some macrochromosomes, including the Z chromosome. The most evolutionary notable finding was a Robertsonian translocation between the microchromosome 17 and the Z chromosome, a rare event in birds. Additionally, the simple short repeats (SSRs) tested here were preferentially accumulated in the microchromosomes and in the Z and W chromosomes, showing no relationship with the constitutive heterochromatin regions, except in the W chromosome. Taken together, our results suggest that the avian sex chromosome is more complex than previously postulated and revealed the role of microchromosomes in the avian sex chromosome evolution, especially cuckoos. MDPI 2020-12-22 /pmc/articles/PMC7822047/ /pubmed/33375072 http://dx.doi.org/10.3390/cells10010004 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Kretschmer, Rafael Gunski, Ricardo José Garnero, Analía del Valle de Freitas, Thales Renato Ochotorena Toma, Gustavo Akira Cioffi, Marcelo de Bello de Oliveira, Edivaldo Herculano Corrêa O’Connor, Rebecca E. Griffin, Darren K. Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation |
title | Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation |
title_full | Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation |
title_fullStr | Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation |
title_full_unstemmed | Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation |
title_short | Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation |
title_sort | chromosomal analysis in crotophaga ani (aves, cuculiformes) reveals extensive genomic reorganization and an unusual z-autosome robertsonian translocation |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822047/ https://www.ncbi.nlm.nih.gov/pubmed/33375072 http://dx.doi.org/10.3390/cells10010004 |
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