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Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation

Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined...

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Autores principales: Kretschmer, Rafael, Gunski, Ricardo José, Garnero, Analía del Valle, de Freitas, Thales Renato Ochotorena, Toma, Gustavo Akira, Cioffi, Marcelo de Bello, de Oliveira, Edivaldo Herculano Corrêa, O’Connor, Rebecca E., Griffin, Darren K.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822047/
https://www.ncbi.nlm.nih.gov/pubmed/33375072
http://dx.doi.org/10.3390/cells10010004
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author Kretschmer, Rafael
Gunski, Ricardo José
Garnero, Analía del Valle
de Freitas, Thales Renato Ochotorena
Toma, Gustavo Akira
Cioffi, Marcelo de Bello
de Oliveira, Edivaldo Herculano Corrêa
O’Connor, Rebecca E.
Griffin, Darren K.
author_facet Kretschmer, Rafael
Gunski, Ricardo José
Garnero, Analía del Valle
de Freitas, Thales Renato Ochotorena
Toma, Gustavo Akira
Cioffi, Marcelo de Bello
de Oliveira, Edivaldo Herculano Corrêa
O’Connor, Rebecca E.
Griffin, Darren K.
author_sort Kretschmer, Rafael
collection PubMed
description Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined conventional and molecular cytogenetic approaches to investigate chromosome homologies between chicken and the smooth-billed ani (Crotophaga ani). Our results demonstrate extensive chromosome reorganization in C. ani, with interchromosomal rearrangements involving macro and microchromosomes. Intrachromosomal rearrangements were observed in some macrochromosomes, including the Z chromosome. The most evolutionary notable finding was a Robertsonian translocation between the microchromosome 17 and the Z chromosome, a rare event in birds. Additionally, the simple short repeats (SSRs) tested here were preferentially accumulated in the microchromosomes and in the Z and W chromosomes, showing no relationship with the constitutive heterochromatin regions, except in the W chromosome. Taken together, our results suggest that the avian sex chromosome is more complex than previously postulated and revealed the role of microchromosomes in the avian sex chromosome evolution, especially cuckoos.
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spelling pubmed-78220472021-01-23 Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation Kretschmer, Rafael Gunski, Ricardo José Garnero, Analía del Valle de Freitas, Thales Renato Ochotorena Toma, Gustavo Akira Cioffi, Marcelo de Bello de Oliveira, Edivaldo Herculano Corrêa O’Connor, Rebecca E. Griffin, Darren K. Cells Article Although cytogenetics studies in cuckoos (Aves, Cuculiformes) have demonstrated an interesting karyotype variation, such as variations in the chromosome morphology and diploid number, their chromosome organization and evolution, and relation with other birds are poorly understood. Hence, we combined conventional and molecular cytogenetic approaches to investigate chromosome homologies between chicken and the smooth-billed ani (Crotophaga ani). Our results demonstrate extensive chromosome reorganization in C. ani, with interchromosomal rearrangements involving macro and microchromosomes. Intrachromosomal rearrangements were observed in some macrochromosomes, including the Z chromosome. The most evolutionary notable finding was a Robertsonian translocation between the microchromosome 17 and the Z chromosome, a rare event in birds. Additionally, the simple short repeats (SSRs) tested here were preferentially accumulated in the microchromosomes and in the Z and W chromosomes, showing no relationship with the constitutive heterochromatin regions, except in the W chromosome. Taken together, our results suggest that the avian sex chromosome is more complex than previously postulated and revealed the role of microchromosomes in the avian sex chromosome evolution, especially cuckoos. MDPI 2020-12-22 /pmc/articles/PMC7822047/ /pubmed/33375072 http://dx.doi.org/10.3390/cells10010004 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kretschmer, Rafael
Gunski, Ricardo José
Garnero, Analía del Valle
de Freitas, Thales Renato Ochotorena
Toma, Gustavo Akira
Cioffi, Marcelo de Bello
de Oliveira, Edivaldo Herculano Corrêa
O’Connor, Rebecca E.
Griffin, Darren K.
Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
title Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
title_full Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
title_fullStr Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
title_full_unstemmed Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
title_short Chromosomal Analysis in Crotophaga ani (Aves, Cuculiformes) Reveals Extensive Genomic Reorganization and an Unusual Z-Autosome Robertsonian Translocation
title_sort chromosomal analysis in crotophaga ani (aves, cuculiformes) reveals extensive genomic reorganization and an unusual z-autosome robertsonian translocation
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822047/
https://www.ncbi.nlm.nih.gov/pubmed/33375072
http://dx.doi.org/10.3390/cells10010004
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