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Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi

Fusarium species are important seedborne pathogens that cause rice bakanae disease (RBD). In this study, 421 strains were isolated from 25 rice samples collected from Zhejiang, Anhui, and Jiangxi provinces of China. Furthermore, 407 isolates were identified as F. fujikuroi (80.05% isolation frequenc...

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Autores principales: Jiang, Hubiao, Wu, Na, Jin, Shaomin, Ahmed, Temoor, Wang, Hui, Li, Bin, Wu, Xiaobi, Bao, Yidan, Liu, Fei, Zhang, Jing-Ze
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822049/
https://www.ncbi.nlm.nih.gov/pubmed/33374990
http://dx.doi.org/10.3390/pathogens10010001
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author Jiang, Hubiao
Wu, Na
Jin, Shaomin
Ahmed, Temoor
Wang, Hui
Li, Bin
Wu, Xiaobi
Bao, Yidan
Liu, Fei
Zhang, Jing-Ze
author_facet Jiang, Hubiao
Wu, Na
Jin, Shaomin
Ahmed, Temoor
Wang, Hui
Li, Bin
Wu, Xiaobi
Bao, Yidan
Liu, Fei
Zhang, Jing-Ze
author_sort Jiang, Hubiao
collection PubMed
description Fusarium species are important seedborne pathogens that cause rice bakanae disease (RBD). In this study, 421 strains were isolated from 25 rice samples collected from Zhejiang, Anhui, and Jiangxi provinces of China. Furthermore, 407 isolates were identified as F. fujikuroi (80.05% isolation frequency), F. proliferatum (8.31%), F. equiseti (5.94%), F. incarnatum (2.61%), F. andiyazi (0.95%), and F. asiaticum (0.48%) based on morphology and translation elongation factor 1-alpha (TEF1-α) gene. Phylogenetic analysis of combined sequences of the RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2), TEF1-α gene, and ribosomal DNA (rDNA) internal transcribed spacer (ITS) showed that 17 representative strains were attributed to six species. Pathogenicity tests showed that representative isolates possessed varying ability to cause symptoms of bakanae on rice seedlings. Moreover, the seed germination assay revealed that six isolates had different effects, such as inhibition of seed germination, as well as seed and bud rot. The loop mediated isothermal amplification (LAMP)-based assay were developed for the detection of F. fujikuroi. According to sequences of desaturase-coding gene promoter, a species-specific marker desM231 was developed for the detection of F. fujikuroi. The LAMP assay using seeds collected from field was validated, and diagnostics developed are efficient, rapid, and sensitive.
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spelling pubmed-78220492021-01-23 Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi Jiang, Hubiao Wu, Na Jin, Shaomin Ahmed, Temoor Wang, Hui Li, Bin Wu, Xiaobi Bao, Yidan Liu, Fei Zhang, Jing-Ze Pathogens Article Fusarium species are important seedborne pathogens that cause rice bakanae disease (RBD). In this study, 421 strains were isolated from 25 rice samples collected from Zhejiang, Anhui, and Jiangxi provinces of China. Furthermore, 407 isolates were identified as F. fujikuroi (80.05% isolation frequency), F. proliferatum (8.31%), F. equiseti (5.94%), F. incarnatum (2.61%), F. andiyazi (0.95%), and F. asiaticum (0.48%) based on morphology and translation elongation factor 1-alpha (TEF1-α) gene. Phylogenetic analysis of combined sequences of the RNA polymerase II largest subunit (RPB1), RNA polymerase II second largest subunit (RPB2), TEF1-α gene, and ribosomal DNA (rDNA) internal transcribed spacer (ITS) showed that 17 representative strains were attributed to six species. Pathogenicity tests showed that representative isolates possessed varying ability to cause symptoms of bakanae on rice seedlings. Moreover, the seed germination assay revealed that six isolates had different effects, such as inhibition of seed germination, as well as seed and bud rot. The loop mediated isothermal amplification (LAMP)-based assay were developed for the detection of F. fujikuroi. According to sequences of desaturase-coding gene promoter, a species-specific marker desM231 was developed for the detection of F. fujikuroi. The LAMP assay using seeds collected from field was validated, and diagnostics developed are efficient, rapid, and sensitive. MDPI 2020-12-22 /pmc/articles/PMC7822049/ /pubmed/33374990 http://dx.doi.org/10.3390/pathogens10010001 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Jiang, Hubiao
Wu, Na
Jin, Shaomin
Ahmed, Temoor
Wang, Hui
Li, Bin
Wu, Xiaobi
Bao, Yidan
Liu, Fei
Zhang, Jing-Ze
Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi
title Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi
title_full Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi
title_fullStr Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi
title_full_unstemmed Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi
title_short Identification of Rice Seed-Derived Fusarium spp. and Development of LAMP Assay against Fusarium fujikuroi
title_sort identification of rice seed-derived fusarium spp. and development of lamp assay against fusarium fujikuroi
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822049/
https://www.ncbi.nlm.nih.gov/pubmed/33374990
http://dx.doi.org/10.3390/pathogens10010001
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