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A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches

Paraquat is a highly toxic herbicide. Paraquat poisoning is often fatal and is an important public health threat in many places. The quick identification and timely initiation of treatment based on timely analysis of the paraquat concentration in urine/serum could improve the prognosis for patients....

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Autores principales: Wang, Tse-Yao, Lee, Yi-Tzu, Chen, Hsien-Yi, Ko, Cheng-Hao, Hong, Chi-Tsung, Wen, Jyun-Wei, Yen, Tzung-Hai, Cheng, Chao-Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822122/
https://www.ncbi.nlm.nih.gov/pubmed/33375112
http://dx.doi.org/10.3390/diagnostics11010006
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author Wang, Tse-Yao
Lee, Yi-Tzu
Chen, Hsien-Yi
Ko, Cheng-Hao
Hong, Chi-Tsung
Wen, Jyun-Wei
Yen, Tzung-Hai
Cheng, Chao-Min
author_facet Wang, Tse-Yao
Lee, Yi-Tzu
Chen, Hsien-Yi
Ko, Cheng-Hao
Hong, Chi-Tsung
Wen, Jyun-Wei
Yen, Tzung-Hai
Cheng, Chao-Min
author_sort Wang, Tse-Yao
collection PubMed
description Paraquat is a highly toxic herbicide. Paraquat poisoning is often fatal and is an important public health threat in many places. The quick identification and timely initiation of treatment based on timely analysis of the paraquat concentration in urine/serum could improve the prognosis for patients. However, current paraquat concentration measurements are time-consuming and difficult to implement due to the expensive and bulky equipment required. To address these practical challenges, paper-based devices have emerged as alternative diagnostic tools for improving point-of-care testing. In this study, we demonstrate the successful use of a paper-based analytical device for the accurate detection of urine paraquat concentration. The developed paper-based analytical device employs colorimetric paraquat concentration measurements. The R(2) value for the urine paraquat standard curve was 0.9989, with a dynamic range of 0–100 ppm. The limit of detection was 3.01 ppm. Two other optical-based approaches, Spectrochip and NanoDrop, were used for comparison. The results suggest that the developed paper-based analytical device is comparable to other colorimetric measurements, as determined by Bland–Altman analysis. The device was clinically validated using urine from six paraquat-poisoned patients. The results prove that the developed paper-based analytical device is accurate, easy-to-use, and efficient for urine paraquat concentration measurement, and may enable physicians to improve clinical management.
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spelling pubmed-78221222021-01-23 A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches Wang, Tse-Yao Lee, Yi-Tzu Chen, Hsien-Yi Ko, Cheng-Hao Hong, Chi-Tsung Wen, Jyun-Wei Yen, Tzung-Hai Cheng, Chao-Min Diagnostics (Basel) Article Paraquat is a highly toxic herbicide. Paraquat poisoning is often fatal and is an important public health threat in many places. The quick identification and timely initiation of treatment based on timely analysis of the paraquat concentration in urine/serum could improve the prognosis for patients. However, current paraquat concentration measurements are time-consuming and difficult to implement due to the expensive and bulky equipment required. To address these practical challenges, paper-based devices have emerged as alternative diagnostic tools for improving point-of-care testing. In this study, we demonstrate the successful use of a paper-based analytical device for the accurate detection of urine paraquat concentration. The developed paper-based analytical device employs colorimetric paraquat concentration measurements. The R(2) value for the urine paraquat standard curve was 0.9989, with a dynamic range of 0–100 ppm. The limit of detection was 3.01 ppm. Two other optical-based approaches, Spectrochip and NanoDrop, were used for comparison. The results suggest that the developed paper-based analytical device is comparable to other colorimetric measurements, as determined by Bland–Altman analysis. The device was clinically validated using urine from six paraquat-poisoned patients. The results prove that the developed paper-based analytical device is accurate, easy-to-use, and efficient for urine paraquat concentration measurement, and may enable physicians to improve clinical management. MDPI 2020-12-22 /pmc/articles/PMC7822122/ /pubmed/33375112 http://dx.doi.org/10.3390/diagnostics11010006 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Tse-Yao
Lee, Yi-Tzu
Chen, Hsien-Yi
Ko, Cheng-Hao
Hong, Chi-Tsung
Wen, Jyun-Wei
Yen, Tzung-Hai
Cheng, Chao-Min
A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches
title A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches
title_full A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches
title_fullStr A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches
title_full_unstemmed A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches
title_short A Paper-Based Analytical Device for Analysis of Paraquat in Urine and Its Validation with Optical-Based Approaches
title_sort paper-based analytical device for analysis of paraquat in urine and its validation with optical-based approaches
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822122/
https://www.ncbi.nlm.nih.gov/pubmed/33375112
http://dx.doi.org/10.3390/diagnostics11010006
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