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Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants

Atopic dermatitis is accompanied by the abnormal overgrowth of Staphylococcus aureus, a common cause of skin infections and an opportunistic pathogen. Although administration of antibiotics is effective against S. aureus, the resulting reduction in healthy microbiota and the emergence of drug-resist...

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Autores principales: Shimamori, Yuzuki, Mitsunaka, Shoichi, Yamashita, Hirotaka, Suzuki, Tohru, Kitao, Tomoe, Kubori, Tomoko, Nagai, Hiroki, Takeda, Shigeki, Ando, Hiroki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822210/
https://www.ncbi.nlm.nih.gov/pubmed/33375201
http://dx.doi.org/10.3390/v13010007
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author Shimamori, Yuzuki
Mitsunaka, Shoichi
Yamashita, Hirotaka
Suzuki, Tohru
Kitao, Tomoe
Kubori, Tomoko
Nagai, Hiroki
Takeda, Shigeki
Ando, Hiroki
author_facet Shimamori, Yuzuki
Mitsunaka, Shoichi
Yamashita, Hirotaka
Suzuki, Tohru
Kitao, Tomoe
Kubori, Tomoko
Nagai, Hiroki
Takeda, Shigeki
Ando, Hiroki
author_sort Shimamori, Yuzuki
collection PubMed
description Atopic dermatitis is accompanied by the abnormal overgrowth of Staphylococcus aureus, a common cause of skin infections and an opportunistic pathogen. Although administration of antibiotics is effective against S. aureus, the resulting reduction in healthy microbiota and the emergence of drug-resistant bacteria are of concern. We propose that phage therapy can be an effective strategy to treat atopic dermatitis without perturbing the microbiota structure. In this study, we examined whether the S. aureus phage SaGU1 could be a tool to counteract the atopic exacerbation induced by S. aureus using an atopic mouse model. Administration of SaGU1 to the back skin of mice reduced both S. aureus counts and the disease exacerbation caused by S. aureus. Furthermore, the S. aureus-mediated exacerbation of atopic dermatitis with respect to IgE plasma concentration and histopathological findings was ameliorated by the application of SaGU1. We also found that Staphylococcus epidermidis, a typical epidermal symbiont in healthy skin, significantly attenuated the emergence of SaGU1-resistant S. aureus under co-culture with S. aureus and S. epidermidis in liquid culture infection experiments. Our results suggest that phage therapy using SaGU1 could be a promising clinical treatment for atopic dermatitis.
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spelling pubmed-78222102021-01-23 Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants Shimamori, Yuzuki Mitsunaka, Shoichi Yamashita, Hirotaka Suzuki, Tohru Kitao, Tomoe Kubori, Tomoko Nagai, Hiroki Takeda, Shigeki Ando, Hiroki Viruses Article Atopic dermatitis is accompanied by the abnormal overgrowth of Staphylococcus aureus, a common cause of skin infections and an opportunistic pathogen. Although administration of antibiotics is effective against S. aureus, the resulting reduction in healthy microbiota and the emergence of drug-resistant bacteria are of concern. We propose that phage therapy can be an effective strategy to treat atopic dermatitis without perturbing the microbiota structure. In this study, we examined whether the S. aureus phage SaGU1 could be a tool to counteract the atopic exacerbation induced by S. aureus using an atopic mouse model. Administration of SaGU1 to the back skin of mice reduced both S. aureus counts and the disease exacerbation caused by S. aureus. Furthermore, the S. aureus-mediated exacerbation of atopic dermatitis with respect to IgE plasma concentration and histopathological findings was ameliorated by the application of SaGU1. We also found that Staphylococcus epidermidis, a typical epidermal symbiont in healthy skin, significantly attenuated the emergence of SaGU1-resistant S. aureus under co-culture with S. aureus and S. epidermidis in liquid culture infection experiments. Our results suggest that phage therapy using SaGU1 could be a promising clinical treatment for atopic dermatitis. MDPI 2020-12-22 /pmc/articles/PMC7822210/ /pubmed/33375201 http://dx.doi.org/10.3390/v13010007 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shimamori, Yuzuki
Mitsunaka, Shoichi
Yamashita, Hirotaka
Suzuki, Tohru
Kitao, Tomoe
Kubori, Tomoko
Nagai, Hiroki
Takeda, Shigeki
Ando, Hiroki
Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants
title Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants
title_full Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants
title_fullStr Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants
title_full_unstemmed Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants
title_short Staphylococcal Phage in Combination with Staphylococcus epidermidis as a Potential Treatment for Staphylococcus aureus-Associated Atopic Dermatitis and Suppressor of Phage-Resistant Mutants
title_sort staphylococcal phage in combination with staphylococcus epidermidis as a potential treatment for staphylococcus aureus-associated atopic dermatitis and suppressor of phage-resistant mutants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822210/
https://www.ncbi.nlm.nih.gov/pubmed/33375201
http://dx.doi.org/10.3390/v13010007
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