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Glyoxal fixation facilitates transcriptome analysis after antigen staining and cell sorting by flow cytometry
A simple method for extraction of high quality RNA from cells that have been fixed, stained and sorted by flow cytometry would allow routine transcriptome analysis of highly purified cell populations and single cells. However, formaldehyde fixation impairs RNA extraction and inhibits RNA amplificati...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
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Public Library of Science
2021
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822327/ https://www.ncbi.nlm.nih.gov/pubmed/33481798 http://dx.doi.org/10.1371/journal.pone.0240769 |