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Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves

Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and...

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Autores principales: Zvikas, Vaidotas, Urbanaviciute, Ieva, Bernotiene, Rasa, Kulakauskiene, Deimante, Morkunaite, Urte, Balion, Zbigniev, Majiene, Daiva, Liaudanskas, Mindaugas, Viskelis, Pranas, Jekabsone, Aiste, Jakstas, Valdas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822480/
https://www.ncbi.nlm.nih.gov/pubmed/33374689
http://dx.doi.org/10.3390/foods10010018
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author Zvikas, Vaidotas
Urbanaviciute, Ieva
Bernotiene, Rasa
Kulakauskiene, Deimante
Morkunaite, Urte
Balion, Zbigniev
Majiene, Daiva
Liaudanskas, Mindaugas
Viskelis, Pranas
Jekabsone, Aiste
Jakstas, Valdas
author_facet Zvikas, Vaidotas
Urbanaviciute, Ieva
Bernotiene, Rasa
Kulakauskiene, Deimante
Morkunaite, Urte
Balion, Zbigniev
Majiene, Daiva
Liaudanskas, Mindaugas
Viskelis, Pranas
Jekabsone, Aiste
Jakstas, Valdas
author_sort Zvikas, Vaidotas
collection PubMed
description Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ leaf extracts on glioblastoma C6 and HROG36 cells. As identified by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry, the extracts contained three prevailing groups of phenols: hydroxycinnamic acid derivatives; flavan-3-ols; and flavonols. Sixteen phenols were detected; the predominant compound was chlorogenic acid. The sum of detected phenols varied significantly between the cultivars ranging from 9322 µg/g (‘Rondo’) to 17,048 µg/g DW (‘Darius’). Incubation with the extracts decreased the viability of glioblastoma HROG36 cells with an efficiency similar to temozolomide, a drug used for glioblastoma treatment. In the case of C6 glioblastoma cells, the extracts were even more efficient than temozolomide. Interestingly, primary cerebellar neuronal-glial cells were significantly less sensitive to the extracts compared to the cancer cell lines. The results showed that JQ leaf ethanol extracts are rich in phenolic compounds, can efficiently reduce glioblastoma cell viability while preserving non-cancerous cells, and are worth further investigations as potential anticancer drugs.
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spelling pubmed-78224802021-01-23 Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves Zvikas, Vaidotas Urbanaviciute, Ieva Bernotiene, Rasa Kulakauskiene, Deimante Morkunaite, Urte Balion, Zbigniev Majiene, Daiva Liaudanskas, Mindaugas Viskelis, Pranas Jekabsone, Aiste Jakstas, Valdas Foods Article Glioblastoma multiforme is an aggressive and invasive disease with no efficient therapy available, and there is a great need for finding alternative treatment strategies. This study aimed to investigate anticancer activity of the extracts of the Japanese quince (JQ) cultivars ‘Darius’, ‘Rondo’, and ‘Rasa’ leaf extracts on glioblastoma C6 and HROG36 cells. As identified by ultra high performance liquid chromatography electrospray ionization tandem mass spectrometry, the extracts contained three prevailing groups of phenols: hydroxycinnamic acid derivatives; flavan-3-ols; and flavonols. Sixteen phenols were detected; the predominant compound was chlorogenic acid. The sum of detected phenols varied significantly between the cultivars ranging from 9322 µg/g (‘Rondo’) to 17,048 µg/g DW (‘Darius’). Incubation with the extracts decreased the viability of glioblastoma HROG36 cells with an efficiency similar to temozolomide, a drug used for glioblastoma treatment. In the case of C6 glioblastoma cells, the extracts were even more efficient than temozolomide. Interestingly, primary cerebellar neuronal-glial cells were significantly less sensitive to the extracts compared to the cancer cell lines. The results showed that JQ leaf ethanol extracts are rich in phenolic compounds, can efficiently reduce glioblastoma cell viability while preserving non-cancerous cells, and are worth further investigations as potential anticancer drugs. MDPI 2020-12-23 /pmc/articles/PMC7822480/ /pubmed/33374689 http://dx.doi.org/10.3390/foods10010018 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zvikas, Vaidotas
Urbanaviciute, Ieva
Bernotiene, Rasa
Kulakauskiene, Deimante
Morkunaite, Urte
Balion, Zbigniev
Majiene, Daiva
Liaudanskas, Mindaugas
Viskelis, Pranas
Jekabsone, Aiste
Jakstas, Valdas
Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
title Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
title_full Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
title_fullStr Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
title_full_unstemmed Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
title_short Investigation of Phenolic Composition and Anticancer Properties of Ethanolic Extracts of Japanese Quince Leaves
title_sort investigation of phenolic composition and anticancer properties of ethanolic extracts of japanese quince leaves
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822480/
https://www.ncbi.nlm.nih.gov/pubmed/33374689
http://dx.doi.org/10.3390/foods10010018
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