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Estrogen is required for maintaining the quality of cardiac stem cells

Compared to the age-matched men, the incidence of cardiovascular diseases is lower in premenopausal but higher in postmenopausal women, suggesting the cardio-protective role of estrogen in females. Although cardiac stem cells (CSCs) express estrogen receptors, yet the effects of estrogen on CSCs rem...

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Autores principales: Hasan, Al Shaimaa, Luo, Lan, Baba, Satoko, Li, Tao-Sheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822545/
https://www.ncbi.nlm.nih.gov/pubmed/33481861
http://dx.doi.org/10.1371/journal.pone.0245166
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author Hasan, Al Shaimaa
Luo, Lan
Baba, Satoko
Li, Tao-Sheng
author_facet Hasan, Al Shaimaa
Luo, Lan
Baba, Satoko
Li, Tao-Sheng
author_sort Hasan, Al Shaimaa
collection PubMed
description Compared to the age-matched men, the incidence of cardiovascular diseases is lower in premenopausal but higher in postmenopausal women, suggesting the cardio-protective role of estrogen in females. Although cardiac stem cells (CSCs) express estrogen receptors, yet the effects of estrogen on CSCs remain unclear. In this study, we investigated the potential role of estrogen in maintaining the quality of CSCs by in vivo and in vitro experiments. For the in vivo study, estrogen deficiency was induced by ovariectomy in 6-weeks-old C57BL/6 female mice, and then randomly given 17β-estradiol (E(2)) replacements at a low dose (0.01 mg/60 days) and high dose (0.18 mg/60 days), or vehicle treatment. All mice were killed 2 months after treatments, and heart tissues were collected for ex vivo expansion of CSCs. Compared to age-matched healthy controls, estrogen deficiency slightly decreased the yield of CSCs with significantly lower telomerase activity and more DNA damage. Interestingly, E(2) replacements at low and high doses significantly increased the yield of CSCs and reversed the quality impairment of CSCs following estrogen deficiency. For the in vitro study, twice-passaged CSCs from the hearts of adult healthy female mice were cultured with the supplement of 0.01, 0.1, and 1 μM E(2) in the medium for 3 days. We found that E(2) supplement increased c-kit expression, increased proliferative activity, improved telomerase activity, and reduced DNA damage of CSCs in a dose-dependent manner. Our data suggested the potential role of estrogen in maintaining the quality of CSCs, providing new insight into the cardio-protective effects of estrogen.
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spelling pubmed-78225452021-02-01 Estrogen is required for maintaining the quality of cardiac stem cells Hasan, Al Shaimaa Luo, Lan Baba, Satoko Li, Tao-Sheng PLoS One Research Article Compared to the age-matched men, the incidence of cardiovascular diseases is lower in premenopausal but higher in postmenopausal women, suggesting the cardio-protective role of estrogen in females. Although cardiac stem cells (CSCs) express estrogen receptors, yet the effects of estrogen on CSCs remain unclear. In this study, we investigated the potential role of estrogen in maintaining the quality of CSCs by in vivo and in vitro experiments. For the in vivo study, estrogen deficiency was induced by ovariectomy in 6-weeks-old C57BL/6 female mice, and then randomly given 17β-estradiol (E(2)) replacements at a low dose (0.01 mg/60 days) and high dose (0.18 mg/60 days), or vehicle treatment. All mice were killed 2 months after treatments, and heart tissues were collected for ex vivo expansion of CSCs. Compared to age-matched healthy controls, estrogen deficiency slightly decreased the yield of CSCs with significantly lower telomerase activity and more DNA damage. Interestingly, E(2) replacements at low and high doses significantly increased the yield of CSCs and reversed the quality impairment of CSCs following estrogen deficiency. For the in vitro study, twice-passaged CSCs from the hearts of adult healthy female mice were cultured with the supplement of 0.01, 0.1, and 1 μM E(2) in the medium for 3 days. We found that E(2) supplement increased c-kit expression, increased proliferative activity, improved telomerase activity, and reduced DNA damage of CSCs in a dose-dependent manner. Our data suggested the potential role of estrogen in maintaining the quality of CSCs, providing new insight into the cardio-protective effects of estrogen. Public Library of Science 2021-01-22 /pmc/articles/PMC7822545/ /pubmed/33481861 http://dx.doi.org/10.1371/journal.pone.0245166 Text en © 2021 Hasan et al http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Hasan, Al Shaimaa
Luo, Lan
Baba, Satoko
Li, Tao-Sheng
Estrogen is required for maintaining the quality of cardiac stem cells
title Estrogen is required for maintaining the quality of cardiac stem cells
title_full Estrogen is required for maintaining the quality of cardiac stem cells
title_fullStr Estrogen is required for maintaining the quality of cardiac stem cells
title_full_unstemmed Estrogen is required for maintaining the quality of cardiac stem cells
title_short Estrogen is required for maintaining the quality of cardiac stem cells
title_sort estrogen is required for maintaining the quality of cardiac stem cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822545/
https://www.ncbi.nlm.nih.gov/pubmed/33481861
http://dx.doi.org/10.1371/journal.pone.0245166
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