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Structure Analysis and Antioxidant Activity of a Novel Polysaccharide from Katan Seeds

In the present work, a novel water-soluble polysaccharide (LWSP) was purified from Katan seeds. Polysaccharide was structurally characterized by NMR spectroscopic analysis, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR)...

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Detalles Bibliográficos
Autores principales: Trabelsi, Imen, Ben Slima, Sirine, Ktari, Naourez, Bouaziz, Mohamed, Ben Salah, Riadh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7822655/
https://www.ncbi.nlm.nih.gov/pubmed/33511204
http://dx.doi.org/10.1155/2021/6349019
Descripción
Sumario:In the present work, a novel water-soluble polysaccharide (LWSP) was purified from Katan seeds. Polysaccharide was structurally characterized by NMR spectroscopic analysis, thin-layer chromatography (TLC), high-performance liquid chromatography (HPLC), Fourier-transform infrared spectroscopy (FTIR) analysis, X-ray diffraction (XRD), and UV absorption. TLC and HPLC showed that LWSP was a polysaccharide consisted mainly of glucose, mannose, xylose, and arabinose. The FTIR spectrum and UV absorption proved polysaccharide characteristic of LWSP. According to XRD, LWSP presented a semicrystalline behavior. The molecular weight was estimated as 64.56 kDa. Results obtained through (13)C and (1)H nuclear magnetic resonance (NMR) indicated that LWSP is consisted of four monosaccharide residues with α and β anomers. Physicochemical and antioxidant properties of LWSP were also investigated. Results revealed that LWSP exhibited interesting 1,1-diphenyl-2-picrylhydrazyl (DPPH) (IC(50) = 4.48 mg/ml) and chelating activity (IC(50) = 4.79 mg/ml), and it displayed moderate reductive capacities. Overall, the findings suggested that LWSP is a promising source of natural additives in various industries fields.