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A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis
BACKGROUND: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) for...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823175/ https://www.ncbi.nlm.nih.gov/pubmed/33485335 http://dx.doi.org/10.1186/s12917-021-02760-w |
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author | Kong, Yufang Wang, Huiyu Wu, Shaoqiang Lv, Jizhou Mei, Lin Zhou, Huifang Lin, Xiangmei Han, Xueqing |
author_facet | Kong, Yufang Wang, Huiyu Wu, Shaoqiang Lv, Jizhou Mei, Lin Zhou, Huifang Lin, Xiangmei Han, Xueqing |
author_sort | Kong, Yufang |
collection | PubMed |
description | BACKGROUND: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum. RESULTS: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The critical threshold for determining negative or positive through the ratio of the fluorescent signal of the test line and the control line (H(T) / H(C)) is 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICST and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, the sensitivity of RBPT is 1:32, and no cross reaction with the sera of other related diseases was observed. CONCLUSION: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost. |
format | Online Article Text |
id | pubmed-7823175 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-78231752021-01-25 A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis Kong, Yufang Wang, Huiyu Wu, Shaoqiang Lv, Jizhou Mei, Lin Zhou, Huifang Lin, Xiangmei Han, Xueqing BMC Vet Res Research Article BACKGROUND: Brucellosis is a serious zoonosis disease that frequently causes significant economic loss in animal husbandry and threatens human health. Therefore, we established a rapid, accurate, simple and sensitive fluorescent immunochromatographic strip test (ICST) based on quantum dots (QDs) for detection the antibodies of Brucella infection animals serum. RESULTS: The test strips were successfully prepared by quantum dot fluorescent microspheres (QDFM) as tracers, which were covalently coupled to an outer membrane protein of Brucella OMP22. The outer membrane protein OMP28 and monoclonal antibodies of OMP22 were separately dispensed onto a nitrocellulose membrane as test and quality control lines, respectively. The critical threshold for determining negative or positive through the ratio of the fluorescent signal of the test line and the control line (H(T) / H(C)) is 0.0492. The repeatability was excellent with an overall average CV of 8.78%. Under optimum conditions, the limit of detection was 1.05 ng/mL (1:512 dilution). With regard to the detection of brucellosis in 150 clinical samples, the total coincidence rate of ICST and Rose Bengal plate test (RBPT) was 97.3%, the coincidence rate of positive samples was 98.8%, the coincidence rate of negative samples was 95.3%, the sensitivity of RBPT is 1:32, and no cross reaction with the sera of other related diseases was observed. CONCLUSION: In our present study, the QDFM has promising application for on-site screening of brucellosis owing to its high detection speed, high sensitivity, high specificity and low cost. BioMed Central 2021-01-23 /pmc/articles/PMC7823175/ /pubmed/33485335 http://dx.doi.org/10.1186/s12917-021-02760-w Text en © The Author(s) 2021 Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Article Kong, Yufang Wang, Huiyu Wu, Shaoqiang Lv, Jizhou Mei, Lin Zhou, Huifang Lin, Xiangmei Han, Xueqing A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
title | A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
title_full | A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
title_fullStr | A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
title_full_unstemmed | A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
title_short | A quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
title_sort | quantum dot fluorescent microsphere based immunochromatographic strip for detection of brucellosis |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823175/ https://www.ncbi.nlm.nih.gov/pubmed/33485335 http://dx.doi.org/10.1186/s12917-021-02760-w |
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