Cargando…

The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice

Phenotypic health effects, both positive and negative, have been well studied in association with the consumption of alcohol in humans as well as several other mammals including mice. Many studies have also associated these same health effects and phenotypes to specific members of gut microbiome com...

Descripción completa

Detalles Bibliográficos
Autores principales: LeBrun, Erick S., Nighot, Meghali, Dharmaprakash, Viszwapriya, Kumar, Anand, Lo, Chien-Chi, Chain, Patrick S. G., Ma, Thomas Y.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823357/
https://www.ncbi.nlm.nih.gov/pubmed/33374112
http://dx.doi.org/10.3390/life11010007
_version_ 1783639816531869696
author LeBrun, Erick S.
Nighot, Meghali
Dharmaprakash, Viszwapriya
Kumar, Anand
Lo, Chien-Chi
Chain, Patrick S. G.
Ma, Thomas Y.
author_facet LeBrun, Erick S.
Nighot, Meghali
Dharmaprakash, Viszwapriya
Kumar, Anand
Lo, Chien-Chi
Chain, Patrick S. G.
Ma, Thomas Y.
author_sort LeBrun, Erick S.
collection PubMed
description Phenotypic health effects, both positive and negative, have been well studied in association with the consumption of alcohol in humans as well as several other mammals including mice. Many studies have also associated these same health effects and phenotypes to specific members of gut microbiome communities. Here we utilized a chronic plus binge ethanol feed model (Gao-binge model) to explore microbiome community changes across three independent experiments performed in mice. We found significant and reproducible differences in microbiome community assemblies between ethanol-treated mice and control mice on the same diet absent of ethanol. We also identified significant differences in gut microbiota occurring temporally with ethanol treatment. Peak shift in communities was observed 4 days after the start of daily alcohol consumption. We quantitatively identified many of the bacterial genera indicative of these ethanol-induced shifts including 20 significant genera when comparing ethanol treatments with controls and 14 significant genera based on temporal investigation. Including overlap of treatment with temporal shifts, we identified 25 specific genera of interest in ethanol treatment microbiome shifts. Shifts coincide with observed presentation of fatty deposits in the liver tissue, i.e., Alcoholic Liver Disease-associated phenotype. The evidence presented herein, derived from three independent experiments, points to the existence of a common, reproducible, and characterizable “mouse ethanol gut microbiome”.
format Online
Article
Text
id pubmed-7823357
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78233572021-01-24 The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice LeBrun, Erick S. Nighot, Meghali Dharmaprakash, Viszwapriya Kumar, Anand Lo, Chien-Chi Chain, Patrick S. G. Ma, Thomas Y. Life (Basel) Article Phenotypic health effects, both positive and negative, have been well studied in association with the consumption of alcohol in humans as well as several other mammals including mice. Many studies have also associated these same health effects and phenotypes to specific members of gut microbiome communities. Here we utilized a chronic plus binge ethanol feed model (Gao-binge model) to explore microbiome community changes across three independent experiments performed in mice. We found significant and reproducible differences in microbiome community assemblies between ethanol-treated mice and control mice on the same diet absent of ethanol. We also identified significant differences in gut microbiota occurring temporally with ethanol treatment. Peak shift in communities was observed 4 days after the start of daily alcohol consumption. We quantitatively identified many of the bacterial genera indicative of these ethanol-induced shifts including 20 significant genera when comparing ethanol treatments with controls and 14 significant genera based on temporal investigation. Including overlap of treatment with temporal shifts, we identified 25 specific genera of interest in ethanol treatment microbiome shifts. Shifts coincide with observed presentation of fatty deposits in the liver tissue, i.e., Alcoholic Liver Disease-associated phenotype. The evidence presented herein, derived from three independent experiments, points to the existence of a common, reproducible, and characterizable “mouse ethanol gut microbiome”. MDPI 2020-12-24 /pmc/articles/PMC7823357/ /pubmed/33374112 http://dx.doi.org/10.3390/life11010007 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
LeBrun, Erick S.
Nighot, Meghali
Dharmaprakash, Viszwapriya
Kumar, Anand
Lo, Chien-Chi
Chain, Patrick S. G.
Ma, Thomas Y.
The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice
title The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice
title_full The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice
title_fullStr The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice
title_full_unstemmed The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice
title_short The Gut Microbiome and Alcoholic Liver Disease: Ethanol Consumption Drives Consistent and Reproducible Alteration in Gut Microbiota in Mice
title_sort gut microbiome and alcoholic liver disease: ethanol consumption drives consistent and reproducible alteration in gut microbiota in mice
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823357/
https://www.ncbi.nlm.nih.gov/pubmed/33374112
http://dx.doi.org/10.3390/life11010007
work_keys_str_mv AT lebrunericks thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT nighotmeghali thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT dharmaprakashviszwapriya thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT kumaranand thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT lochienchi thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT chainpatricksg thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT mathomasy thegutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT lebrunericks gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT nighotmeghali gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT dharmaprakashviszwapriya gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT kumaranand gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT lochienchi gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT chainpatricksg gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice
AT mathomasy gutmicrobiomeandalcoholicliverdiseaseethanolconsumptiondrivesconsistentandreproduciblealterationingutmicrobiotainmice