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Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs

Measuring antibodies to evaluate dogs’ immunity against canine parvovirus (CPV) is useful to avoid unnecessary re-vaccinations. The study aimed to evaluate the quality and practicability of four point-of-care (POC) tests for detection of anti-CPV antibodies. The sera of 198 client-owned and 43 speci...

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Autores principales: Bergmann, Michèle, Holzheu, Mike, Zablotski, Yury, Speck, Stephanie, Truyen, Uwe, Straubinger, Reinhard K., Hartmann, Katrin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823389/
https://www.ncbi.nlm.nih.gov/pubmed/33374843
http://dx.doi.org/10.3390/v13010018
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author Bergmann, Michèle
Holzheu, Mike
Zablotski, Yury
Speck, Stephanie
Truyen, Uwe
Straubinger, Reinhard K.
Hartmann, Katrin
author_facet Bergmann, Michèle
Holzheu, Mike
Zablotski, Yury
Speck, Stephanie
Truyen, Uwe
Straubinger, Reinhard K.
Hartmann, Katrin
author_sort Bergmann, Michèle
collection PubMed
description Measuring antibodies to evaluate dogs’ immunity against canine parvovirus (CPV) is useful to avoid unnecessary re-vaccinations. The study aimed to evaluate the quality and practicability of four point-of-care (POC) tests for detection of anti-CPV antibodies. The sera of 198 client-owned and 43 specific pathogen-free (SPF) dogs were included; virus neutralization was the reference method. Specificity, sensitivity, positive and negative predictive value (PPV and NPV), and overall accuracy (OA) were calculated. Specificity was considered to be the most important indicator for POC test performance. Differences between specificity and sensitivity of POC tests in the sera of all dogs were determined by McNemar, agreement by Cohen’s kappa. Prevalence of anti-CPV antibodies in all dogs was 80% (192/241); in the subgroup of client-owned dogs, it was 97% (192/198); and in the subgroup of SPF dogs, it was 0% (0/43). FASTest(®) and CanTiCheck(®) were easiest to perform. Specificity was highest in the CanTiCheck(®) (overall dogs, 98%; client-owned dogs, 83%; SPF dogs, 100%) and the TiterCHEK(®) (overall dogs, 96%; client-owned dogs, 67%; SPF dogs, 100%); no significant differences in specificity were observed between the ImmunoComb(®), the TiterCHEK(®), and the CanTiCheck(®). Sensitivity was highest in the FASTest(®) (overall dogs, 95%; client-owned dogs, 95%) and the CanTiCheck(®) (overall dogs, 80%; client-owned dogs, 80%); sensitivity of the FASTest(®) was significantly higher compared to the one of the other three tests (McNemars p-value in each comparison: <0.001). CanTiCheck(®) would be the POC test of choice when considering specificity and practicability. However, differences in the number of false positive results between CanTiCheck(®), TiterCHEK(®), and ImmunoComb(®) were minimal.
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spelling pubmed-78233892021-01-24 Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs Bergmann, Michèle Holzheu, Mike Zablotski, Yury Speck, Stephanie Truyen, Uwe Straubinger, Reinhard K. Hartmann, Katrin Viruses Article Measuring antibodies to evaluate dogs’ immunity against canine parvovirus (CPV) is useful to avoid unnecessary re-vaccinations. The study aimed to evaluate the quality and practicability of four point-of-care (POC) tests for detection of anti-CPV antibodies. The sera of 198 client-owned and 43 specific pathogen-free (SPF) dogs were included; virus neutralization was the reference method. Specificity, sensitivity, positive and negative predictive value (PPV and NPV), and overall accuracy (OA) were calculated. Specificity was considered to be the most important indicator for POC test performance. Differences between specificity and sensitivity of POC tests in the sera of all dogs were determined by McNemar, agreement by Cohen’s kappa. Prevalence of anti-CPV antibodies in all dogs was 80% (192/241); in the subgroup of client-owned dogs, it was 97% (192/198); and in the subgroup of SPF dogs, it was 0% (0/43). FASTest(®) and CanTiCheck(®) were easiest to perform. Specificity was highest in the CanTiCheck(®) (overall dogs, 98%; client-owned dogs, 83%; SPF dogs, 100%) and the TiterCHEK(®) (overall dogs, 96%; client-owned dogs, 67%; SPF dogs, 100%); no significant differences in specificity were observed between the ImmunoComb(®), the TiterCHEK(®), and the CanTiCheck(®). Sensitivity was highest in the FASTest(®) (overall dogs, 95%; client-owned dogs, 95%) and the CanTiCheck(®) (overall dogs, 80%; client-owned dogs, 80%); sensitivity of the FASTest(®) was significantly higher compared to the one of the other three tests (McNemars p-value in each comparison: <0.001). CanTiCheck(®) would be the POC test of choice when considering specificity and practicability. However, differences in the number of false positive results between CanTiCheck(®), TiterCHEK(®), and ImmunoComb(®) were minimal. MDPI 2020-12-23 /pmc/articles/PMC7823389/ /pubmed/33374843 http://dx.doi.org/10.3390/v13010018 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Bergmann, Michèle
Holzheu, Mike
Zablotski, Yury
Speck, Stephanie
Truyen, Uwe
Straubinger, Reinhard K.
Hartmann, Katrin
Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs
title Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs
title_full Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs
title_fullStr Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs
title_full_unstemmed Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs
title_short Comparison of Four Commercially Available Point-of-Care Tests to Detect Antibodies against Canine Parvovirus in Dogs
title_sort comparison of four commercially available point-of-care tests to detect antibodies against canine parvovirus in dogs
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823389/
https://www.ncbi.nlm.nih.gov/pubmed/33374843
http://dx.doi.org/10.3390/v13010018
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