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Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome

Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in t...

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Autores principales: Ra, Sang Hyun, Kim, Min Jae, Kim, Min-Chul, Park, Se Yoon, Park, Seong Yeon, Chong, Yong Pil, Lee, Sang-Oh, Choi, Sang-Ho, Kim, Yang Soo, Lee, Keun Hwa, Kim, Sung-Han, Kee, Sun-Ho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823500/
https://www.ncbi.nlm.nih.gov/pubmed/33375753
http://dx.doi.org/10.3390/v13010006
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author Ra, Sang Hyun
Kim, Min Jae
Kim, Min-Chul
Park, Se Yoon
Park, Seong Yeon
Chong, Yong Pil
Lee, Sang-Oh
Choi, Sang-Ho
Kim, Yang Soo
Lee, Keun Hwa
Kim, Sung-Han
Kee, Sun-Ho
author_facet Ra, Sang Hyun
Kim, Min Jae
Kim, Min-Chul
Park, Se Yoon
Park, Seong Yeon
Chong, Yong Pil
Lee, Sang-Oh
Choi, Sang-Ho
Kim, Yang Soo
Lee, Keun Hwa
Kim, Sung-Han
Kee, Sun-Ho
author_sort Ra, Sang Hyun
collection PubMed
description Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS.
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spelling pubmed-78235002021-01-24 Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome Ra, Sang Hyun Kim, Min Jae Kim, Min-Chul Park, Se Yoon Park, Seong Yeon Chong, Yong Pil Lee, Sang-Oh Choi, Sang-Ho Kim, Yang Soo Lee, Keun Hwa Kim, Sung-Han Kee, Sun-Ho Viruses Brief Report Severe fever with thrombocytopenia syndrome (SFTS) is caused by SFTS virus (SFTSV). We investigated the detailed kinetics of serologic response in patients with SFTS. Twenty-eight patients aged ≥18 years were enrolled between July 2015 and October 2018. SFTS was confirmed by detecting SFTSV RNA in their plasma using reverse transcription polymerase chain reaction. SFTSV-specific IgG and IgM were measured using immunofluorescence assay (IFA) and enzyme-linked immunosorbent assay (ELISA). We found that SFTSV-specific IgG was detected at days 5–9 after symptom onset, and its titer was rising during the course of disease. SFTSV-specific IgM titer peaked at around week 2–3 from symptom onset. The SFTSV-specific seropositive rates for days 5–9, 10–14, 15–19, and 20–24 from symptom onset using IFA and ELISA were 63%, 76%, 90%, and 100%, and 58%, 86%, 100%, and 100%, respectively, for IgG, whereas they were 32%, 62%, 80%, and 100%, and 53%, 62%, 70%, and 100%, respectively, for IgM. The delayed IgM response could be attributed to the low sensitivity of SFTSV-specific IgM IFA or ELISA and/or impaired immune responses. The IgM test using IFA or ELISA that we used in this study is, therefore, insufficient for the early diagnosis of SFTS. MDPI 2020-12-25 /pmc/articles/PMC7823500/ /pubmed/33375753 http://dx.doi.org/10.3390/v13010006 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
Ra, Sang Hyun
Kim, Min Jae
Kim, Min-Chul
Park, Se Yoon
Park, Seong Yeon
Chong, Yong Pil
Lee, Sang-Oh
Choi, Sang-Ho
Kim, Yang Soo
Lee, Keun Hwa
Kim, Sung-Han
Kee, Sun-Ho
Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome
title Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome
title_full Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome
title_fullStr Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome
title_full_unstemmed Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome
title_short Kinetics of Serological Response in Patients with Severe Fever with Thrombocytopenia Syndrome
title_sort kinetics of serological response in patients with severe fever with thrombocytopenia syndrome
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823500/
https://www.ncbi.nlm.nih.gov/pubmed/33375753
http://dx.doi.org/10.3390/v13010006
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