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Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants

Carotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato (Ipomoea batatas [L.] Lam) Orange gene (IbOr-R96H), which carries a single nucleotide polymorphism responsible...

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Autores principales: Kim, So-Eun, Lee, Chan-Ju, Park, Sul-U, Lim, Ye-Hoon, Park, Woo Sung, Kim, Hye-Jin, Ahn, Mi-Jeong, Kwak, Sang-Soo, Kim, Ho Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823567/
https://www.ncbi.nlm.nih.gov/pubmed/33406723
http://dx.doi.org/10.3390/antiox10010051
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author Kim, So-Eun
Lee, Chan-Ju
Park, Sul-U
Lim, Ye-Hoon
Park, Woo Sung
Kim, Hye-Jin
Ahn, Mi-Jeong
Kwak, Sang-Soo
Kim, Ho Soo
author_facet Kim, So-Eun
Lee, Chan-Ju
Park, Sul-U
Lim, Ye-Hoon
Park, Woo Sung
Kim, Hye-Jin
Ahn, Mi-Jeong
Kwak, Sang-Soo
Kim, Ho Soo
author_sort Kim, So-Eun
collection PubMed
description Carotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato (Ipomoea batatas [L.] Lam) Orange gene (IbOr-R96H), which carries a single nucleotide polymorphism responsible for Arg to His substitution at amino acid position 96, exhibited dramatically higher carotenoid content and abiotic stress tolerance than calli overexpressing the wild-type IbOr gene (IbOr-WT). In this study, we generated transgenic sweetpotato plants overexpressing IbOr-R96H under the control of the cauliflower mosaic virus (CaMV) 35S promoter via Agrobacterium-mediated transformation. The total carotenoid contents of IbOr-R96H storage roots (light-orange flesh) and IbOr-WT storage roots (light-yellow flesh) were 5.4–19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). The β-carotene content of IbOr-R96H storage roots was up to 186.2-fold higher than that of NT storage roots. In addition, IbOr-R96H plants showed greater tolerance to heat stress (47 °C) than NT and IbOr-WT plants, possibly because of higher DPPH radical scavenging activity and ABA contents. These results indicate that IbOr-R96H is a promising strategy for developing new sweetpotato cultivars with improved carotenoid contents and heat stress tolerance.
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spelling pubmed-78235672021-01-24 Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants Kim, So-Eun Lee, Chan-Ju Park, Sul-U Lim, Ye-Hoon Park, Woo Sung Kim, Hye-Jin Ahn, Mi-Jeong Kwak, Sang-Soo Kim, Ho Soo Antioxidants (Basel) Article Carotenoids function as photosynthetic accessory pigments, antioxidants, and vitamin A precursors. We recently showed that transgenic sweetpotato calli overexpressing the mutant sweetpotato (Ipomoea batatas [L.] Lam) Orange gene (IbOr-R96H), which carries a single nucleotide polymorphism responsible for Arg to His substitution at amino acid position 96, exhibited dramatically higher carotenoid content and abiotic stress tolerance than calli overexpressing the wild-type IbOr gene (IbOr-WT). In this study, we generated transgenic sweetpotato plants overexpressing IbOr-R96H under the control of the cauliflower mosaic virus (CaMV) 35S promoter via Agrobacterium-mediated transformation. The total carotenoid contents of IbOr-R96H storage roots (light-orange flesh) and IbOr-WT storage roots (light-yellow flesh) were 5.4–19.6 and 3.2-fold higher, respectively, than those of non-transgenic (NT) storage roots (white flesh). The β-carotene content of IbOr-R96H storage roots was up to 186.2-fold higher than that of NT storage roots. In addition, IbOr-R96H plants showed greater tolerance to heat stress (47 °C) than NT and IbOr-WT plants, possibly because of higher DPPH radical scavenging activity and ABA contents. These results indicate that IbOr-R96H is a promising strategy for developing new sweetpotato cultivars with improved carotenoid contents and heat stress tolerance. MDPI 2021-01-04 /pmc/articles/PMC7823567/ /pubmed/33406723 http://dx.doi.org/10.3390/antiox10010051 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, So-Eun
Lee, Chan-Ju
Park, Sul-U
Lim, Ye-Hoon
Park, Woo Sung
Kim, Hye-Jin
Ahn, Mi-Jeong
Kwak, Sang-Soo
Kim, Ho Soo
Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants
title Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants
title_full Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants
title_fullStr Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants
title_full_unstemmed Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants
title_short Overexpression of the Golden SNP-Carrying Orange Gene Enhances Carotenoid Accumulation and Heat Stress Tolerance in Sweetpotato Plants
title_sort overexpression of the golden snp-carrying orange gene enhances carotenoid accumulation and heat stress tolerance in sweetpotato plants
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823567/
https://www.ncbi.nlm.nih.gov/pubmed/33406723
http://dx.doi.org/10.3390/antiox10010051
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