Cargando…

Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm

Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the ci...

Descripción completa

Detalles Bibliográficos
Autores principales: Machado, Thamiris Santana, Pinheiro, Felipe Ramos, Andre, Lialyz Soares Pereira, Pereira, Renata Freire Alves, Correa, Reginaldo Fernandes, de Mello, Gabriela Coutinho, Ribeiro, Tainara Aparecida Nunes, Penna, Bruno, Sachs, Daniela, Aguiar-Alves, Fábio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823648/
https://www.ncbi.nlm.nih.gov/pubmed/33375552
http://dx.doi.org/10.3390/toxins13010014
_version_ 1783639885774585856
author Machado, Thamiris Santana
Pinheiro, Felipe Ramos
Andre, Lialyz Soares Pereira
Pereira, Renata Freire Alves
Correa, Reginaldo Fernandes
de Mello, Gabriela Coutinho
Ribeiro, Tainara Aparecida Nunes
Penna, Bruno
Sachs, Daniela
Aguiar-Alves, Fábio
author_facet Machado, Thamiris Santana
Pinheiro, Felipe Ramos
Andre, Lialyz Soares Pereira
Pereira, Renata Freire Alves
Correa, Reginaldo Fernandes
de Mello, Gabriela Coutinho
Ribeiro, Tainara Aparecida Nunes
Penna, Bruno
Sachs, Daniela
Aguiar-Alves, Fábio
author_sort Machado, Thamiris Santana
collection PubMed
description Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection.
format Online
Article
Text
id pubmed-7823648
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78236482021-01-24 Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm Machado, Thamiris Santana Pinheiro, Felipe Ramos Andre, Lialyz Soares Pereira Pereira, Renata Freire Alves Correa, Reginaldo Fernandes de Mello, Gabriela Coutinho Ribeiro, Tainara Aparecida Nunes Penna, Bruno Sachs, Daniela Aguiar-Alves, Fábio Toxins (Basel) Article Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection. MDPI 2020-12-25 /pmc/articles/PMC7823648/ /pubmed/33375552 http://dx.doi.org/10.3390/toxins13010014 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Machado, Thamiris Santana
Pinheiro, Felipe Ramos
Andre, Lialyz Soares Pereira
Pereira, Renata Freire Alves
Correa, Reginaldo Fernandes
de Mello, Gabriela Coutinho
Ribeiro, Tainara Aparecida Nunes
Penna, Bruno
Sachs, Daniela
Aguiar-Alves, Fábio
Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
title Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
title_full Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
title_fullStr Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
title_full_unstemmed Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
title_short Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
title_sort virulence factors found in nasal colonization and infection of methicillin-resistant staphylococcus aureus (mrsa) isolates and their ability to form a biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823648/
https://www.ncbi.nlm.nih.gov/pubmed/33375552
http://dx.doi.org/10.3390/toxins13010014
work_keys_str_mv AT machadothamirissantana virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT pinheirofeliperamos virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT andrelialyzsoarespereira virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT pereirarenatafreirealves virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT correareginaldofernandes virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT demellogabrielacoutinho virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT ribeirotainaraaparecidanunes virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT pennabruno virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT sachsdaniela virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm
AT aguiaralvesfabio virulencefactorsfoundinnasalcolonizationandinfectionofmethicillinresistantstaphylococcusaureusmrsaisolatesandtheirabilitytoformabiofilm