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Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm
Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the ci...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823648/ https://www.ncbi.nlm.nih.gov/pubmed/33375552 http://dx.doi.org/10.3390/toxins13010014 |
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author | Machado, Thamiris Santana Pinheiro, Felipe Ramos Andre, Lialyz Soares Pereira Pereira, Renata Freire Alves Correa, Reginaldo Fernandes de Mello, Gabriela Coutinho Ribeiro, Tainara Aparecida Nunes Penna, Bruno Sachs, Daniela Aguiar-Alves, Fábio |
author_facet | Machado, Thamiris Santana Pinheiro, Felipe Ramos Andre, Lialyz Soares Pereira Pereira, Renata Freire Alves Correa, Reginaldo Fernandes de Mello, Gabriela Coutinho Ribeiro, Tainara Aparecida Nunes Penna, Bruno Sachs, Daniela Aguiar-Alves, Fábio |
author_sort | Machado, Thamiris Santana |
collection | PubMed |
description | Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection. |
format | Online Article Text |
id | pubmed-7823648 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78236482021-01-24 Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm Machado, Thamiris Santana Pinheiro, Felipe Ramos Andre, Lialyz Soares Pereira Pereira, Renata Freire Alves Correa, Reginaldo Fernandes de Mello, Gabriela Coutinho Ribeiro, Tainara Aparecida Nunes Penna, Bruno Sachs, Daniela Aguiar-Alves, Fábio Toxins (Basel) Article Hospitalizations related to Methicillin-resistant Staphylococcus aureus (MRSA) are frequent, increasing mortality and health costs. In this way, this study aimed to compare the genotypic and phenotypic characteristics of MRSA isolates that colonize and infect patients seen at two hospitals in the city of Niterói—Rio de Janeiro, Brazil. A total of 147 samples collected between March 2013 and December 2015 were phenotyped and genotyped to identify the protein A (SPA) gene, the mec staphylococcal chromosomal cassette (SCCmec), mecA, Panton-Valentine Leucocidin (PVL), icaC, icaR, ACME, and hla virulence genes. The strength of biofilm formation has also been exploited. The prevalence of SCCmec type IV (77.1%) was observed in the colonization group; however, in the invasive infection group, SCCmec type II was prevalent (62.9%). The Multilocus Sequence Typing (MLST), ST5/ST30, and ST5/ST239 analyses were the most frequent clones in colonization, and invasive infection isolates, respectively. Among the isolates selected to assess the ability to form a biofilm, 51.06% were classified as strong biofilm builders. Surprisingly, we observed that isolates other than the Brazilian Epidemic Clone (BEC) have appeared in Brazilian hospitals. The virulence profile has changed among these isolates since the ACME type I and II genes were also identified in this collection. MDPI 2020-12-25 /pmc/articles/PMC7823648/ /pubmed/33375552 http://dx.doi.org/10.3390/toxins13010014 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Machado, Thamiris Santana Pinheiro, Felipe Ramos Andre, Lialyz Soares Pereira Pereira, Renata Freire Alves Correa, Reginaldo Fernandes de Mello, Gabriela Coutinho Ribeiro, Tainara Aparecida Nunes Penna, Bruno Sachs, Daniela Aguiar-Alves, Fábio Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm |
title | Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm |
title_full | Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm |
title_fullStr | Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm |
title_full_unstemmed | Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm |
title_short | Virulence Factors Found in Nasal Colonization and Infection of Methicillin-Resistant Staphylococcus aureus (MRSA) Isolates and Their Ability to Form a Biofilm |
title_sort | virulence factors found in nasal colonization and infection of methicillin-resistant staphylococcus aureus (mrsa) isolates and their ability to form a biofilm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823648/ https://www.ncbi.nlm.nih.gov/pubmed/33375552 http://dx.doi.org/10.3390/toxins13010014 |
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