Cargando…
Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients
Emerging evidence indicates that reactivation of BK polyomavirus (BKPyV) in the kidney and urothelial tract of kidney transplant recipients (KTRs) may be associated with cancer in these sites. In this retrospective study of a single center cohort of KTRs (n = 1307), 10 clear cell renal cell carcinom...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823775/ https://www.ncbi.nlm.nih.gov/pubmed/33401589 http://dx.doi.org/10.3390/v13010056 |
_version_ | 1783639916358402048 |
---|---|
author | Borgogna, Cinzia Albertini, Silvia Martuscelli, Licia Poletti, Filippo Volpe, Alessandro Merlotti, Guido Cantaluppi, Vicenzo Boldorini, Renzo Gariglio, Marisa |
author_facet | Borgogna, Cinzia Albertini, Silvia Martuscelli, Licia Poletti, Filippo Volpe, Alessandro Merlotti, Guido Cantaluppi, Vicenzo Boldorini, Renzo Gariglio, Marisa |
author_sort | Borgogna, Cinzia |
collection | PubMed |
description | Emerging evidence indicates that reactivation of BK polyomavirus (BKPyV) in the kidney and urothelial tract of kidney transplant recipients (KTRs) may be associated with cancer in these sites. In this retrospective study of a single center cohort of KTRs (n = 1307), 10 clear cell renal cell carcinomas and 5 urinary bladder carcinomas were analyzed from 15 KTRs for the presence of BKPyV infection through immunohistochemistry and fluorescent in situ hybridization (FISH). Three of these patients had already exhibited biopsy-proven polyomavirus-associated nephropathies (PyVAN). Although the presence of BKPyV large-T antigen was evident in the urothelium from a kidney removed soon after PyVAN diagnosis, it was undetectable in all the formalin-fixed and paraffin-embedded (FFPE) blocks obtained from the 10 kidney tumors. By contrast, large-T antigen (LT) labeling of tumor cells was detected in two out of five bladder carcinomas. Lastly, the proportion of BKPyV DNA-FISH-positive bladder carcinoma nuclei was much lower than that of LT-positive cells. Taken together, our findings further strengthen the association between BKPyV reactivation and cancer development in KTRs, especially bladder carcinoma. |
format | Online Article Text |
id | pubmed-7823775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78237752021-01-24 Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients Borgogna, Cinzia Albertini, Silvia Martuscelli, Licia Poletti, Filippo Volpe, Alessandro Merlotti, Guido Cantaluppi, Vicenzo Boldorini, Renzo Gariglio, Marisa Viruses Brief Report Emerging evidence indicates that reactivation of BK polyomavirus (BKPyV) in the kidney and urothelial tract of kidney transplant recipients (KTRs) may be associated with cancer in these sites. In this retrospective study of a single center cohort of KTRs (n = 1307), 10 clear cell renal cell carcinomas and 5 urinary bladder carcinomas were analyzed from 15 KTRs for the presence of BKPyV infection through immunohistochemistry and fluorescent in situ hybridization (FISH). Three of these patients had already exhibited biopsy-proven polyomavirus-associated nephropathies (PyVAN). Although the presence of BKPyV large-T antigen was evident in the urothelium from a kidney removed soon after PyVAN diagnosis, it was undetectable in all the formalin-fixed and paraffin-embedded (FFPE) blocks obtained from the 10 kidney tumors. By contrast, large-T antigen (LT) labeling of tumor cells was detected in two out of five bladder carcinomas. Lastly, the proportion of BKPyV DNA-FISH-positive bladder carcinoma nuclei was much lower than that of LT-positive cells. Taken together, our findings further strengthen the association between BKPyV reactivation and cancer development in KTRs, especially bladder carcinoma. MDPI 2021-01-03 /pmc/articles/PMC7823775/ /pubmed/33401589 http://dx.doi.org/10.3390/v13010056 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Brief Report Borgogna, Cinzia Albertini, Silvia Martuscelli, Licia Poletti, Filippo Volpe, Alessandro Merlotti, Guido Cantaluppi, Vicenzo Boldorini, Renzo Gariglio, Marisa Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients |
title | Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients |
title_full | Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients |
title_fullStr | Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients |
title_full_unstemmed | Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients |
title_short | Evidence of BK Polyomavirus Infection in Urothelial but not Renal Tumors from a Single Center Cohort of Kidney Transplant Recipients |
title_sort | evidence of bk polyomavirus infection in urothelial but not renal tumors from a single center cohort of kidney transplant recipients |
topic | Brief Report |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823775/ https://www.ncbi.nlm.nih.gov/pubmed/33401589 http://dx.doi.org/10.3390/v13010056 |
work_keys_str_mv | AT borgognacinzia evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT albertinisilvia evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT martuscellilicia evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT polettifilippo evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT volpealessandro evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT merlottiguido evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT cantaluppivicenzo evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT boldorinirenzo evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients AT garigliomarisa evidenceofbkpolyomavirusinfectioninurothelialbutnotrenaltumorsfromasinglecentercohortofkidneytransplantrecipients |