Cargando…

β-Galactosidases from a Sequence-Based Metagenome: Cloning, Expression, Purification and Characterization

Stabilization ponds are a common treatment technology for wastewater generated by dairy industries. Large proportions of cheese whey are thrown into these ponds, creating an environmental problem because of the large volume produced and the high biological and chemical oxygen demands. Due to its com...

Descripción completa

Detalles Bibliográficos
Autores principales:	Eberhardt, María Florencia, Irazoqui, José Matías, Amadio, Ariel Fernando
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	MDPI 2020
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823827/ https://www.ncbi.nlm.nih.gov/pubmed/33379234 http://dx.doi.org/10.3390/microorganisms9010055

Ejemplares similares

Cloning, Expression, Purification, and Characterization of β-Galactosidase from Bifidobacterium longum and Bifidobacterium pseudocatenulatum
por: Du, Mingzhu, et al.
Publicado: (2022)

Identification of key microorganisms in facultative stabilization ponds from dairy industries, using metagenomics
por: Irazoqui, Jose M., et al.
Publicado: (2022)

Metagenomic identification, purification and characterisation of the Bifidobacterium adolescentis BgaC β-galactosidase
por: Mulualem, Daniel Mehabie, et al.
Publicado: (2021)

Cloning, overexpression, purification, and characterization of a polyextremophilic β-galactosidase from the Antarctic haloarchaeon Halorubrum lacusprofundi
por: Karan, Ram, et al.
Publicado: (2013)

Cloning, Expression, Purification, and Characterization of a Novel β-Galactosidase/α-L-Arabinopyranosidase from Paenibacillus polymyxa KF-1
por: Cui, Jing, et al.
Publicado: (2023)

A novel cold-active β-D-galactosidase from the Paracoccus sp. 32d - gene cloning, purification and characterization
por: Wierzbicka-Woś, Anna, et al.
Publicado: (2011)

Functional metagenomics reveals novel β-galactosidases not predictable from gene sequences
por: Cheng, Jiujun, et al.
Publicado: (2017)

Dealing with different methods for Kluyveromyces lactis β-galactosidase purification
por: Becerra, M, et al.
Publicado: (1998)

Reconstruction and Characterization of Full-Length Begomovirus and Alphasatellite Genomes Infecting Pepper through Metagenomics
por: Bornancini, Verónica A., et al.
Publicado: (2020)

A new cold-adapted β-D-galactosidase from the Antarctic Arthrobacter sp. 32c – gene cloning, overexpression, purification and properties
por: Hildebrandt, Piotr, et al.
Publicado: (2009)

Cloning, Expression and Characterization of a Novel Cold-adapted β-galactosidase from the Deep-sea Bacterium Alteromonas sp. ML52
por: Sun, Jingjing, et al.
Publicado: (2018)

Expression Cloning of Recombinant Escherichia coli lacZ Genes Encoding Cytoplasmic and Nuclear β-galactosidase Variants
por: Naderian, Homayoun, et al.
Publicado: (2011)

Cloning and characterization of a novel cold-active glycoside hydrolase family 1 enzyme with β-glucosidase, β-fucosidase and β-galactosidase activities
por: Wierzbicka-Woś, Anna, et al.
Publicado: (2013)

Genomic analysis of shiga toxin-containing Escherichia coli O157:H7 isolated from Argentinean cattle
por: Amadio, Ariel, et al.
Publicado: (2021)

Two β-Galactosidases from the Human Isolate Bifidobacterium breve DSM 20213: Molecular Cloning and Expression, Biochemical Characterization and Synthesis of Galacto-Oligosaccharides
por: Arreola, Sheryl Lozel, et al.
Publicado: (2014)

Purification and characterisation of intracellular alpha-galactosidases from Acinetobacter sp.
por: E, Sirisha, et al.
Publicado: (2015)

Synthesis of Oligosaccharides Derived from Lactulose (OsLu) Using Soluble and Immobilized Aspergillus oryzae β-Galactosidase
por: Cardelle-Cobas, Alejandra, et al.
Publicado: (2016)

Identification, Characterization, and Expression of a β-Galactosidase from Arion Species (Mollusca)
por: Thoma, Julia, et al.
Publicado: (2022)

Cloning and Characterization of a New β-Galactosidase from Alteromonas sp. QD01 and Its Potential in Synthesis of Galacto-Oligosaccharides
por: Li, Dandan, et al.
Publicado: (2020)

Cloning, Purification, and Partial Characterization of Bacillus subtilis Urate Oxidase Expressed in Escherichia coli
por: Pfrimer, Pollyana, et al.
Publicado: (2010)

Cloning, expression, purification and characterization of human mitochondrial carbonic anhydrase VA
por: Idrees, Danish, et al.
Publicado: (2016)

Quantitative digital in situ senescence-associated β-galactosidase assay
por: Shlush, Liran I, et al.
Publicado: (2011)

Cloning, sequencing, expression, and purification of SARS-associated coronavirus nucleocapsid protein for serodiagnosis of SARS
por: Timani, Khalid Amine, et al.
Publicado: (2004)

Heterologous expression and characterization of an Arabidopsis β-l-arabinopyranosidase and α-d-galactosidases acting on β-l-arabinopyranosyl residues
por: Imaizumi, Chiemi, et al.
Publicado: (2017)

Molecular Cloning, Expression, Purification, and Functional Characterization of Dammarenediol Synthase from Panax ginseng
por: Hu, Wei, et al.
Publicado: (2013)

Cloning, Purification and Characterization of the Collagenase ColA Expressed by Bacillus cereus ATCC 14579
por: Abfalter, Carmen M., et al.
Publicado: (2016)

Cloning, Expression, and Purification of Pseudomonas aeruginosa Flagellin, and Characterization of the Elicited Anti-Flagellin Antibody
por: Behrouz, Bahador, et al.
Publicado: (2016)

Identification and characterization of a novel β-D-galactosidase that releases pyruvylated galactose
por: Higuchi, Yujiro, et al.
Publicado: (2018)

BDNF Promoter–Mediated β-Galactosidase Expression in the Olfactory Epithelium and Bulb
por: Clevenger, Amy C., et al.
Publicado: (2008)

Cloning and expression of a novel α-galactosidase from Lactobacillus amylolyticus L6 with hydrolytic and transgalactosyl properties
por: Fei, Yongtao, et al.
Publicado: (2020)

Cloning, Expression, and Purification of the Nucleocapsid Protein of SARS Coronavirus
por: Netesova, N. A., et al.
Publicado: (2004)

Cloning, expression and purification of recombinant dermatopontin in Escherichia coli
por: Seetaraman Amritha, Trikkur Madom, et al.
Publicado: (2020)

Protocol for high-throughput cloning, expression, purification, and evaluation of bispecific antibodies
por: Li, Danqing, et al.
Publicado: (2022)

β-galactosidase stability at high substrate concentrations
por: Warmerdam, Anja, et al.
Publicado: (2013)

Senescence-Associated β-Galactosidase Detection in Pathology
por: Valieva, Yana, et al.
Publicado: (2022)

Phylogenomic analysis for Campylobacter fetus ocurring in Argentina
por: Farace, Pablo Daniel, et al.
Publicado: (2021)

Metagenomic approach for the isolation of a thermostable β-galactosidase with high tolerance of galactose and glucose from soil samples of Turpan Basin
por: Zhang, Xia, et al.
Publicado: (2013)

Release of β-galactosidase from poloxamine/α-cyclodextrin hydrogels
por: Estévez, César A, et al.
Publicado: (2014)

β-Galactosidase from Lactobacillus helveticus DSM 20075: Biochemical Characterization and Recombinant Expression for Applications in Dairy Industry
por: Kittibunchakul, Suwapat, et al.
Publicado: (2019)

Molecular cloning, expression, purification and functional characterization of an antifungal cyclophilin protein from Panax ginseng
por: Zhang, Hui, et al.
Publicado: (2017)

Cannot write session to /tmp/vufind_sessions/sess_a0h97pfseqorhjld8tftodr7ri