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Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria
The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screene...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823855/ https://www.ncbi.nlm.nih.gov/pubmed/33379186 http://dx.doi.org/10.3390/microorganisms9010054 |
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author | Belkessa, Salem Thomas-Lopez, Daniel Houali, Karim Ghalmi, Farida Stensvold, Christen Rune |
author_facet | Belkessa, Salem Thomas-Lopez, Daniel Houali, Karim Ghalmi, Farida Stensvold, Christen Rune |
author_sort | Belkessa, Salem |
collection | PubMed |
description | The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data. |
format | Online Article Text |
id | pubmed-7823855 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78238552021-01-24 Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria Belkessa, Salem Thomas-Lopez, Daniel Houali, Karim Ghalmi, Farida Stensvold, Christen Rune Microorganisms Article The molecular epidemiology of giardiasis in Africa remains unclear. A study was carried out across four hospitals in Algeria. A total of 119 fecal samples from 55 children, 37 adults, and 27 individuals of undetermined age, all scored positive for intestinal parasites by microscopy, and were screened by real-time PCR for Giardia. Molecular characterization of Giardia was performed by assemblage-specific PCR and PCR targeting the triose phosphate isomerase gene (tpi). Of the 119 samples, 80 (67%) were Giardia-positive by real-time PCR. For 48 moderately-highly real-time PCR-positive samples, tpi genotyping assigned 22 samples to Assemblage A and 26 to Assemblage B. Contrary to Assemblage A, Assemblage B exhibited substantial genetic diversity and allelic heterozygosity. Assemblage-specific PCR proved to be specific for discriminating Assemblage A or B but not as sensitive as tpi genotyping. We confirmed that real-time PCR is more sensitive than microscopy for detecting Giardia in stool samples and that robust amplification and sequencing of the tpi gene is feasible when moderate-to-strongly real-time PCR-positive samples are used. This study is one of the few performed in Africa providing genotyping data on Giardia infections in humans. Both assemblages A and B were commonly seen and not associated with specific sociodemographic data. MDPI 2020-12-28 /pmc/articles/PMC7823855/ /pubmed/33379186 http://dx.doi.org/10.3390/microorganisms9010054 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Belkessa, Salem Thomas-Lopez, Daniel Houali, Karim Ghalmi, Farida Stensvold, Christen Rune Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria |
title | Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria |
title_full | Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria |
title_fullStr | Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria |
title_full_unstemmed | Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria |
title_short | Molecular Characterization of Giardia duodenalis in Children and Adults Sampled in Algeria |
title_sort | molecular characterization of giardia duodenalis in children and adults sampled in algeria |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823855/ https://www.ncbi.nlm.nih.gov/pubmed/33379186 http://dx.doi.org/10.3390/microorganisms9010054 |
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