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Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida

The type three secretion system (TTSS) locus of Aeromonas salmonicida subsp. salmonicida, located on the plasmid pAsa5, is known to be lost when the bacterium is grown at temperatures of 25 °C. The loss of the locus is due to the recombination of the insertion sequences flanking the TTSS region. How...

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Autores principales: Marcoux, Pierre-Étienne, Vincent, Antony T., Massicotte, Marie-Ange, Paquet, Valérie E., Doucet, Émilie J., Hosseini, Nava, Trudel, Mélanie V., Byatt, Gabriel, Laurent, Mathilde, Frenette, Michel, Charette, Steve J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823893/
https://www.ncbi.nlm.nih.gov/pubmed/33396556
http://dx.doi.org/10.3390/microorganisms9010085
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author Marcoux, Pierre-Étienne
Vincent, Antony T.
Massicotte, Marie-Ange
Paquet, Valérie E.
Doucet, Émilie J.
Hosseini, Nava
Trudel, Mélanie V.
Byatt, Gabriel
Laurent, Mathilde
Frenette, Michel
Charette, Steve J.
author_facet Marcoux, Pierre-Étienne
Vincent, Antony T.
Massicotte, Marie-Ange
Paquet, Valérie E.
Doucet, Émilie J.
Hosseini, Nava
Trudel, Mélanie V.
Byatt, Gabriel
Laurent, Mathilde
Frenette, Michel
Charette, Steve J.
author_sort Marcoux, Pierre-Étienne
collection PubMed
description The type three secretion system (TTSS) locus of Aeromonas salmonicida subsp. salmonicida, located on the plasmid pAsa5, is known to be lost when the bacterium is grown at temperatures of 25 °C. The loss of the locus is due to the recombination of the insertion sequences flanking the TTSS region. However, the mechanism involved in this recombination is still elusive. Here, we analyzed 22 A. salmonicida subsp. salmonicida strains that had already lost their TTSS locus, and we systematically explored another 47 strains for their susceptibility to lose the same locus when grown at 25 °C. It appeared that strains from Europe were more prone to lose their TTSS locus compared to Canadian strains. More specifically, it was not possible to induce TTSS loss in Canadian strains that have AsaGEI2a, a genomic island, and prophage 3, or in Canadian strains without a genomic island. A comparative genomic approach revealed an almost perfect correlation between the presence of a cluster of genes, not yet characterized, and the susceptibility of various groups of strains to lose their locus. This cluster of genes encodes putative proteins with DNA binding capacity and phage proteins. This discovery creates new opportunities in the study of pAsa5 thermosensitivity.
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spelling pubmed-78238932021-01-24 Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida Marcoux, Pierre-Étienne Vincent, Antony T. Massicotte, Marie-Ange Paquet, Valérie E. Doucet, Émilie J. Hosseini, Nava Trudel, Mélanie V. Byatt, Gabriel Laurent, Mathilde Frenette, Michel Charette, Steve J. Microorganisms Article The type three secretion system (TTSS) locus of Aeromonas salmonicida subsp. salmonicida, located on the plasmid pAsa5, is known to be lost when the bacterium is grown at temperatures of 25 °C. The loss of the locus is due to the recombination of the insertion sequences flanking the TTSS region. However, the mechanism involved in this recombination is still elusive. Here, we analyzed 22 A. salmonicida subsp. salmonicida strains that had already lost their TTSS locus, and we systematically explored another 47 strains for their susceptibility to lose the same locus when grown at 25 °C. It appeared that strains from Europe were more prone to lose their TTSS locus compared to Canadian strains. More specifically, it was not possible to induce TTSS loss in Canadian strains that have AsaGEI2a, a genomic island, and prophage 3, or in Canadian strains without a genomic island. A comparative genomic approach revealed an almost perfect correlation between the presence of a cluster of genes, not yet characterized, and the susceptibility of various groups of strains to lose their locus. This cluster of genes encodes putative proteins with DNA binding capacity and phage proteins. This discovery creates new opportunities in the study of pAsa5 thermosensitivity. MDPI 2020-12-31 /pmc/articles/PMC7823893/ /pubmed/33396556 http://dx.doi.org/10.3390/microorganisms9010085 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Marcoux, Pierre-Étienne
Vincent, Antony T.
Massicotte, Marie-Ange
Paquet, Valérie E.
Doucet, Émilie J.
Hosseini, Nava
Trudel, Mélanie V.
Byatt, Gabriel
Laurent, Mathilde
Frenette, Michel
Charette, Steve J.
Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
title Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
title_full Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
title_fullStr Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
title_full_unstemmed Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
title_short Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
title_sort systematic analysis of the stress-induced genomic instability of type three secretion system in aeromonas salmonicida subsp. salmonicida
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823893/
https://www.ncbi.nlm.nih.gov/pubmed/33396556
http://dx.doi.org/10.3390/microorganisms9010085
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