Cargando…
Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida
The type three secretion system (TTSS) locus of Aeromonas salmonicida subsp. salmonicida, located on the plasmid pAsa5, is known to be lost when the bacterium is grown at temperatures of 25 °C. The loss of the locus is due to the recombination of the insertion sequences flanking the TTSS region. How...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823893/ https://www.ncbi.nlm.nih.gov/pubmed/33396556 http://dx.doi.org/10.3390/microorganisms9010085 |
_version_ | 1783639944968798208 |
---|---|
author | Marcoux, Pierre-Étienne Vincent, Antony T. Massicotte, Marie-Ange Paquet, Valérie E. Doucet, Émilie J. Hosseini, Nava Trudel, Mélanie V. Byatt, Gabriel Laurent, Mathilde Frenette, Michel Charette, Steve J. |
author_facet | Marcoux, Pierre-Étienne Vincent, Antony T. Massicotte, Marie-Ange Paquet, Valérie E. Doucet, Émilie J. Hosseini, Nava Trudel, Mélanie V. Byatt, Gabriel Laurent, Mathilde Frenette, Michel Charette, Steve J. |
author_sort | Marcoux, Pierre-Étienne |
collection | PubMed |
description | The type three secretion system (TTSS) locus of Aeromonas salmonicida subsp. salmonicida, located on the plasmid pAsa5, is known to be lost when the bacterium is grown at temperatures of 25 °C. The loss of the locus is due to the recombination of the insertion sequences flanking the TTSS region. However, the mechanism involved in this recombination is still elusive. Here, we analyzed 22 A. salmonicida subsp. salmonicida strains that had already lost their TTSS locus, and we systematically explored another 47 strains for their susceptibility to lose the same locus when grown at 25 °C. It appeared that strains from Europe were more prone to lose their TTSS locus compared to Canadian strains. More specifically, it was not possible to induce TTSS loss in Canadian strains that have AsaGEI2a, a genomic island, and prophage 3, or in Canadian strains without a genomic island. A comparative genomic approach revealed an almost perfect correlation between the presence of a cluster of genes, not yet characterized, and the susceptibility of various groups of strains to lose their locus. This cluster of genes encodes putative proteins with DNA binding capacity and phage proteins. This discovery creates new opportunities in the study of pAsa5 thermosensitivity. |
format | Online Article Text |
id | pubmed-7823893 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78238932021-01-24 Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida Marcoux, Pierre-Étienne Vincent, Antony T. Massicotte, Marie-Ange Paquet, Valérie E. Doucet, Émilie J. Hosseini, Nava Trudel, Mélanie V. Byatt, Gabriel Laurent, Mathilde Frenette, Michel Charette, Steve J. Microorganisms Article The type three secretion system (TTSS) locus of Aeromonas salmonicida subsp. salmonicida, located on the plasmid pAsa5, is known to be lost when the bacterium is grown at temperatures of 25 °C. The loss of the locus is due to the recombination of the insertion sequences flanking the TTSS region. However, the mechanism involved in this recombination is still elusive. Here, we analyzed 22 A. salmonicida subsp. salmonicida strains that had already lost their TTSS locus, and we systematically explored another 47 strains for their susceptibility to lose the same locus when grown at 25 °C. It appeared that strains from Europe were more prone to lose their TTSS locus compared to Canadian strains. More specifically, it was not possible to induce TTSS loss in Canadian strains that have AsaGEI2a, a genomic island, and prophage 3, or in Canadian strains without a genomic island. A comparative genomic approach revealed an almost perfect correlation between the presence of a cluster of genes, not yet characterized, and the susceptibility of various groups of strains to lose their locus. This cluster of genes encodes putative proteins with DNA binding capacity and phage proteins. This discovery creates new opportunities in the study of pAsa5 thermosensitivity. MDPI 2020-12-31 /pmc/articles/PMC7823893/ /pubmed/33396556 http://dx.doi.org/10.3390/microorganisms9010085 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Marcoux, Pierre-Étienne Vincent, Antony T. Massicotte, Marie-Ange Paquet, Valérie E. Doucet, Émilie J. Hosseini, Nava Trudel, Mélanie V. Byatt, Gabriel Laurent, Mathilde Frenette, Michel Charette, Steve J. Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida |
title | Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida |
title_full | Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida |
title_fullStr | Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida |
title_full_unstemmed | Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida |
title_short | Systematic Analysis of the Stress-Induced Genomic Instability of Type Three Secretion System in Aeromonas salmonicida subsp. salmonicida |
title_sort | systematic analysis of the stress-induced genomic instability of type three secretion system in aeromonas salmonicida subsp. salmonicida |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823893/ https://www.ncbi.nlm.nih.gov/pubmed/33396556 http://dx.doi.org/10.3390/microorganisms9010085 |
work_keys_str_mv | AT marcouxpierreetienne systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT vincentantonyt systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT massicottemarieange systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT paquetvaleriee systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT doucetemiliej systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT hosseininava systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT trudelmelaniev systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT byattgabriel systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT laurentmathilde systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT frenettemichel systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida AT charettestevej systematicanalysisofthestressinducedgenomicinstabilityoftypethreesecretionsysteminaeromonassalmonicidasubspsalmonicida |