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Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823923/ https://www.ncbi.nlm.nih.gov/pubmed/33396529 http://dx.doi.org/10.3390/jpm11010025 |
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author | Hermoso-Durán, Sonia García-Rayado, Guillermo Ceballos-Laita, Laura Sostres, Carlos Vega, Sonia Millastre, Judith Sánchez-Gracia, Oscar Ojeda, Jorge L. Lanas, Ángel Velázquez-Campoy, Adrián Abian, Olga |
author_facet | Hermoso-Durán, Sonia García-Rayado, Guillermo Ceballos-Laita, Laura Sostres, Carlos Vega, Sonia Millastre, Judith Sánchez-Gracia, Oscar Ojeda, Jorge L. Lanas, Ángel Velázquez-Campoy, Adrián Abian, Olga |
author_sort | Hermoso-Durán, Sonia |
collection | PubMed |
description | Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis. |
format | Online Article Text |
id | pubmed-7823923 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78239232021-01-24 Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis Hermoso-Durán, Sonia García-Rayado, Guillermo Ceballos-Laita, Laura Sostres, Carlos Vega, Sonia Millastre, Judith Sánchez-Gracia, Oscar Ojeda, Jorge L. Lanas, Ángel Velázquez-Campoy, Adrián Abian, Olga J Pers Med Article Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis. MDPI 2020-12-31 /pmc/articles/PMC7823923/ /pubmed/33396529 http://dx.doi.org/10.3390/jpm11010025 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Hermoso-Durán, Sonia García-Rayado, Guillermo Ceballos-Laita, Laura Sostres, Carlos Vega, Sonia Millastre, Judith Sánchez-Gracia, Oscar Ojeda, Jorge L. Lanas, Ángel Velázquez-Campoy, Adrián Abian, Olga Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis |
title | Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis |
title_full | Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis |
title_fullStr | Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis |
title_full_unstemmed | Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis |
title_short | Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis |
title_sort | thermal liquid biopsy (tlb) focused on benign and premalignant pancreatic cyst diagnosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823923/ https://www.ncbi.nlm.nih.gov/pubmed/33396529 http://dx.doi.org/10.3390/jpm11010025 |
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