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Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis

Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this...

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Autores principales: Hermoso-Durán, Sonia, García-Rayado, Guillermo, Ceballos-Laita, Laura, Sostres, Carlos, Vega, Sonia, Millastre, Judith, Sánchez-Gracia, Oscar, Ojeda, Jorge L., Lanas, Ángel, Velázquez-Campoy, Adrián, Abian, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823923/
https://www.ncbi.nlm.nih.gov/pubmed/33396529
http://dx.doi.org/10.3390/jpm11010025
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author Hermoso-Durán, Sonia
García-Rayado, Guillermo
Ceballos-Laita, Laura
Sostres, Carlos
Vega, Sonia
Millastre, Judith
Sánchez-Gracia, Oscar
Ojeda, Jorge L.
Lanas, Ángel
Velázquez-Campoy, Adrián
Abian, Olga
author_facet Hermoso-Durán, Sonia
García-Rayado, Guillermo
Ceballos-Laita, Laura
Sostres, Carlos
Vega, Sonia
Millastre, Judith
Sánchez-Gracia, Oscar
Ojeda, Jorge L.
Lanas, Ángel
Velázquez-Campoy, Adrián
Abian, Olga
author_sort Hermoso-Durán, Sonia
collection PubMed
description Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis.
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spelling pubmed-78239232021-01-24 Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis Hermoso-Durán, Sonia García-Rayado, Guillermo Ceballos-Laita, Laura Sostres, Carlos Vega, Sonia Millastre, Judith Sánchez-Gracia, Oscar Ojeda, Jorge L. Lanas, Ángel Velázquez-Campoy, Adrián Abian, Olga J Pers Med Article Background: Current efforts in the identification of new biomarkers are directed towards an accurate differentiation between benign and premalignant cysts. Thermal Liquid Biopsy (TLB) has been previously applied to inflammatory and tumor diseases and could offer an interesting point of view in this type of pathology. Methods: In this work, twenty patients (12 males and 8 females, average ages 62) diagnosed with a pancreatic cyst benign (10) and premalignant (10) cyst lesions were recruited, and biological samples were obtained during the endoscopic ultrasonography procedure. Results: Proteomic content of cyst liquid samples was studied and several common proteins in the different groups were identified. TLB cyst liquid profiles reflected protein content. Also, TLB serum score was able to discriminate between healthy and cysts patients (71% sensitivity and 98% specificity) and between benign and premalignant cysts (75% sensitivity and 67% specificity). Conclusions: TLB analysis of plasmatic serum sample, a quick, simple and non-invasive technique that can be easily implemented, reports valuable information on the observed pancreatic lesion. These preliminary results set the basis for a larger study to refine TLB serum score and move closer to the clinical application of TLB providing useful information to the gastroenterologist during patient diagnosis. MDPI 2020-12-31 /pmc/articles/PMC7823923/ /pubmed/33396529 http://dx.doi.org/10.3390/jpm11010025 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Hermoso-Durán, Sonia
García-Rayado, Guillermo
Ceballos-Laita, Laura
Sostres, Carlos
Vega, Sonia
Millastre, Judith
Sánchez-Gracia, Oscar
Ojeda, Jorge L.
Lanas, Ángel
Velázquez-Campoy, Adrián
Abian, Olga
Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
title Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
title_full Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
title_fullStr Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
title_full_unstemmed Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
title_short Thermal Liquid Biopsy (TLB) Focused on Benign and Premalignant Pancreatic Cyst Diagnosis
title_sort thermal liquid biopsy (tlb) focused on benign and premalignant pancreatic cyst diagnosis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823923/
https://www.ncbi.nlm.nih.gov/pubmed/33396529
http://dx.doi.org/10.3390/jpm11010025
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