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Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro

Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and...

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Autores principales: Schmidt, Jan, Pilbauerova, Nela, Soukup, Tomas, Suchankova-Kleplova, Tereza, Suchanek, Jakub
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823925/
https://www.ncbi.nlm.nih.gov/pubmed/33379324
http://dx.doi.org/10.3390/biom11010022
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author Schmidt, Jan
Pilbauerova, Nela
Soukup, Tomas
Suchankova-Kleplova, Tereza
Suchanek, Jakub
author_facet Schmidt, Jan
Pilbauerova, Nela
Soukup, Tomas
Suchankova-Kleplova, Tereza
Suchanek, Jakub
author_sort Schmidt, Jan
collection PubMed
description Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and biodegradability, it is also a suitable scaffold material. However, low molecular weight (LMW) fragments, produced by enzymatic cleavage of HA, have different bioactive properties to high molecular weight (HMW) HA. Thus, the impact of HA must be assessed separately for each molecular weight fraction. In this study, we present the effect of three LMW-HA fragments (800, 1600, and 15,000 Da) on DPSCs in vitro. Discrete biological parameters such as DPSC viability, morphology, and cell surface marker expression were determined. Following treatment with LMW-HA, DPSCs initially presented with an acute reduction in proliferation (p < 0.0016) and soon recovered in subsequent passages. They displayed significant size reduction (p = 0.0078, p = 0.0019, p = 0.0098) while maintaining high expression of DPSC markers (CD29, CD44, CD73, CD90). However, in contrast to controls, a significant phenotypic shift (p < 0.05; CD29, CD34, CD90, CD106, CD117, CD146, CD166) of surface markers was observed. These findings provide a basis for further detailed investigations and present a strong argument for the importance of HA scaffold degradation kinetics analysis.
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spelling pubmed-78239252021-01-24 Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro Schmidt, Jan Pilbauerova, Nela Soukup, Tomas Suchankova-Kleplova, Tereza Suchanek, Jakub Biomolecules Article Hyaluronic acid (HA) and dental pulp stem cells (DPSCs) are attractive research topics, and their combined use in the field of tissue engineering seems to be very promising. HA is a natural extracellular biopolymer found in various tissues, including dental pulp, and due to its biocompatibility and biodegradability, it is also a suitable scaffold material. However, low molecular weight (LMW) fragments, produced by enzymatic cleavage of HA, have different bioactive properties to high molecular weight (HMW) HA. Thus, the impact of HA must be assessed separately for each molecular weight fraction. In this study, we present the effect of three LMW-HA fragments (800, 1600, and 15,000 Da) on DPSCs in vitro. Discrete biological parameters such as DPSC viability, morphology, and cell surface marker expression were determined. Following treatment with LMW-HA, DPSCs initially presented with an acute reduction in proliferation (p < 0.0016) and soon recovered in subsequent passages. They displayed significant size reduction (p = 0.0078, p = 0.0019, p = 0.0098) while maintaining high expression of DPSC markers (CD29, CD44, CD73, CD90). However, in contrast to controls, a significant phenotypic shift (p < 0.05; CD29, CD34, CD90, CD106, CD117, CD146, CD166) of surface markers was observed. These findings provide a basis for further detailed investigations and present a strong argument for the importance of HA scaffold degradation kinetics analysis. MDPI 2020-12-28 /pmc/articles/PMC7823925/ /pubmed/33379324 http://dx.doi.org/10.3390/biom11010022 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Schmidt, Jan
Pilbauerova, Nela
Soukup, Tomas
Suchankova-Kleplova, Tereza
Suchanek, Jakub
Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
title Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
title_full Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
title_fullStr Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
title_full_unstemmed Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
title_short Low Molecular Weight Hyaluronic Acid Effect on Dental Pulp Stem Cells In Vitro
title_sort low molecular weight hyaluronic acid effect on dental pulp stem cells in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823925/
https://www.ncbi.nlm.nih.gov/pubmed/33379324
http://dx.doi.org/10.3390/biom11010022
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