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Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability

In the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, c...

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Autores principales: Noh, Sung Hyun, Jo, Han-Saem, Choi, Somang, Song, Hee Gyeong, Kim, Hak-Jun, Kim, Keung Nyun, Kim, Sung Eun, Park, Kyeongsoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823981/
https://www.ncbi.nlm.nih.gov/pubmed/33375294
http://dx.doi.org/10.3390/pharmaceutics13010030
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author Noh, Sung Hyun
Jo, Han-Saem
Choi, Somang
Song, Hee Gyeong
Kim, Hak-Jun
Kim, Keung Nyun
Kim, Sung Eun
Park, Kyeongsoon
author_facet Noh, Sung Hyun
Jo, Han-Saem
Choi, Somang
Song, Hee Gyeong
Kim, Hak-Jun
Kim, Keung Nyun
Kim, Sung Eun
Park, Kyeongsoon
author_sort Noh, Sung Hyun
collection PubMed
description In the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, calcium accumulation, and expression of osteo-differentiation-specific genes, including osteocalcin (OCN) and osteopontin (OPN). Both bare MSNs and LF/TA-MSNs exhibited round nano-particle structures. The LF/TA-MSNs demonstrated prolonged LF release for up to 28 days. Treatment of ADSCs with LF (50 μg)/TA-MSNs resulted in markedly higher ALP level and calcium accumulation compared to treatment with LF (10 μg)/TA-MSNs or bare MSNs. Furthermore, LF (50 μg)/TA-MSNs remarkably increased mRNA levels of osteo-differentiation-specific genes, including OCN and OPN, compared to MSNs or LF (10 μg)/TA-MSNs. Together, these data suggest that the ability of LF/TA-MSNs to enhance osteo-differentiation of ADSCs make them a possible nanovehicle for bone healing and bone regeneration in patients with bone defect or disease.
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spelling pubmed-78239812021-01-24 Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability Noh, Sung Hyun Jo, Han-Saem Choi, Somang Song, Hee Gyeong Kim, Hak-Jun Kim, Keung Nyun Kim, Sung Eun Park, Kyeongsoon Pharmaceutics Article In the present study, we created lactoferrin-anchored mesoporous silica nanomaterials with absorbed tannic acid (LF/TA-MSNs) and evaluated the effect of these LF/TA-MSNs on the in vitro osteo-differentiation ability of adipose-derived stem cells (ADSCs) by testing alkaline phosphatase (ALP) level, calcium accumulation, and expression of osteo-differentiation-specific genes, including osteocalcin (OCN) and osteopontin (OPN). Both bare MSNs and LF/TA-MSNs exhibited round nano-particle structures. The LF/TA-MSNs demonstrated prolonged LF release for up to 28 days. Treatment of ADSCs with LF (50 μg)/TA-MSNs resulted in markedly higher ALP level and calcium accumulation compared to treatment with LF (10 μg)/TA-MSNs or bare MSNs. Furthermore, LF (50 μg)/TA-MSNs remarkably increased mRNA levels of osteo-differentiation-specific genes, including OCN and OPN, compared to MSNs or LF (10 μg)/TA-MSNs. Together, these data suggest that the ability of LF/TA-MSNs to enhance osteo-differentiation of ADSCs make them a possible nanovehicle for bone healing and bone regeneration in patients with bone defect or disease. MDPI 2020-12-26 /pmc/articles/PMC7823981/ /pubmed/33375294 http://dx.doi.org/10.3390/pharmaceutics13010030 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Noh, Sung Hyun
Jo, Han-Saem
Choi, Somang
Song, Hee Gyeong
Kim, Hak-Jun
Kim, Keung Nyun
Kim, Sung Eun
Park, Kyeongsoon
Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
title Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
title_full Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
title_fullStr Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
title_full_unstemmed Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
title_short Lactoferrin-Anchored Tannylated Mesoporous Silica Nanomaterials for Enhanced Osteo-Differentiation Ability
title_sort lactoferrin-anchored tannylated mesoporous silica nanomaterials for enhanced osteo-differentiation ability
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7823981/
https://www.ncbi.nlm.nih.gov/pubmed/33375294
http://dx.doi.org/10.3390/pharmaceutics13010030
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