Cargando…

Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity

Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives. The objectives of this study were to assess the phytochemical composition by high-performance liquid chromatography–quadrupole time-of-flight mass/mass spectrom...

Descripción completa

Detalles Bibliográficos
Autores principales: Trujillo-Mayol, Igor, Casas-Forero, Nidia, Pastene-Navarrete, Edgar, Lima Silva, Fabiana, Alarcón-Enos, Julio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824035/
https://www.ncbi.nlm.nih.gov/pubmed/33396588
http://dx.doi.org/10.3390/antibiotics10010023
_version_ 1783639978873454592
author Trujillo-Mayol, Igor
Casas-Forero, Nidia
Pastene-Navarrete, Edgar
Lima Silva, Fabiana
Alarcón-Enos, Julio
author_facet Trujillo-Mayol, Igor
Casas-Forero, Nidia
Pastene-Navarrete, Edgar
Lima Silva, Fabiana
Alarcón-Enos, Julio
author_sort Trujillo-Mayol, Igor
collection PubMed
description Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives. The objectives of this study were to assess the phytochemical composition by high-performance liquid chromatography–quadrupole time-of-flight mass/mass spectrometry (HPLC-qTOF-MS/MS), total phenolic content (TPC), proanthocyanidin (PAC) content, and antioxidant activity of the APE, the organic fraction (OF), the aqueous fraction (AF), and the acid-microwave hydrolyzed APE (HAPE), on the antibacterial activity (ABA). The results indicated that APE and OF contained (p ˂ 0.05) a higher phenolic composition and antioxidant activity than AF and HAPE. The ABA specified that Pseudomonas aeruginosa and Bacillus cereus were inhibited by all the extracts (minimal inhibitory concentration—MIC ≥ 500 µg/mL), Staphylococcus aureus was only significantly inhibited by APE (≥750 µg/mL), the same MIC was observed for the OF on Salmonella spp. and Listeria monocytogenes. The HAPE increased the inhibitory efficiency up to 25% on Escherichia coli and Salmonella spp. (MIC ≥ 750 µg/mL), and 83.34% on L. monocytogenes (MIC ≥ 125 µg/mL) compared to APE (MIC ≥ 750 µg/mL). Also, HAPE inhibited the biofilm formation at the lowest concentration (125 µg/mL); meanwhile, the biofilm disruption showed to be concentration-time-dependent (p ˃ 0.05) compared to amoxicillin. In conclusion, the fractionation and hydrolyzation of APE improved the ABA; thus, those strategies are useful to design new antimicrobial compounds.
format Online
Article
Text
id pubmed-7824035
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78240352021-01-24 Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity Trujillo-Mayol, Igor Casas-Forero, Nidia Pastene-Navarrete, Edgar Lima Silva, Fabiana Alarcón-Enos, Julio Antibiotics (Basel) Article Avocado Hass (Persea americana Mill) peel extract (APE) has the potential as a natural ingredient to substitute for chemical preservatives. The objectives of this study were to assess the phytochemical composition by high-performance liquid chromatography–quadrupole time-of-flight mass/mass spectrometry (HPLC-qTOF-MS/MS), total phenolic content (TPC), proanthocyanidin (PAC) content, and antioxidant activity of the APE, the organic fraction (OF), the aqueous fraction (AF), and the acid-microwave hydrolyzed APE (HAPE), on the antibacterial activity (ABA). The results indicated that APE and OF contained (p ˂ 0.05) a higher phenolic composition and antioxidant activity than AF and HAPE. The ABA specified that Pseudomonas aeruginosa and Bacillus cereus were inhibited by all the extracts (minimal inhibitory concentration—MIC ≥ 500 µg/mL), Staphylococcus aureus was only significantly inhibited by APE (≥750 µg/mL), the same MIC was observed for the OF on Salmonella spp. and Listeria monocytogenes. The HAPE increased the inhibitory efficiency up to 25% on Escherichia coli and Salmonella spp. (MIC ≥ 750 µg/mL), and 83.34% on L. monocytogenes (MIC ≥ 125 µg/mL) compared to APE (MIC ≥ 750 µg/mL). Also, HAPE inhibited the biofilm formation at the lowest concentration (125 µg/mL); meanwhile, the biofilm disruption showed to be concentration-time-dependent (p ˃ 0.05) compared to amoxicillin. In conclusion, the fractionation and hydrolyzation of APE improved the ABA; thus, those strategies are useful to design new antimicrobial compounds. MDPI 2020-12-30 /pmc/articles/PMC7824035/ /pubmed/33396588 http://dx.doi.org/10.3390/antibiotics10010023 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Trujillo-Mayol, Igor
Casas-Forero, Nidia
Pastene-Navarrete, Edgar
Lima Silva, Fabiana
Alarcón-Enos, Julio
Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity
title Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity
title_full Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity
title_fullStr Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity
title_full_unstemmed Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity
title_short Fractionation and Hydrolyzation of Avocado Peel Extract: Improvement of Antibacterial Activity
title_sort fractionation and hydrolyzation of avocado peel extract: improvement of antibacterial activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824035/
https://www.ncbi.nlm.nih.gov/pubmed/33396588
http://dx.doi.org/10.3390/antibiotics10010023
work_keys_str_mv AT trujillomayoligor fractionationandhydrolyzationofavocadopeelextractimprovementofantibacterialactivity
AT casasforeronidia fractionationandhydrolyzationofavocadopeelextractimprovementofantibacterialactivity
AT pastenenavarreteedgar fractionationandhydrolyzationofavocadopeelextractimprovementofantibacterialactivity
AT limasilvafabiana fractionationandhydrolyzationofavocadopeelextractimprovementofantibacterialactivity
AT alarconenosjulio fractionationandhydrolyzationofavocadopeelextractimprovementofantibacterialactivity