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Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization

Madelung Disease (MD) is a syndrome characterized by the accumulation of aberrant symmetric adipose tissue deposits. The etiology of this disease is yet to be elucidated, even though the presence of comorbidities, either genetic or environmental, has been reported. For this reason, establishing an i...

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Autores principales: Caponnetto, Federica, Manini, Ivana, Bulfoni, Michela, Zingaretti, Nicola, Miotti, Giovanni, Di Loreto, Carla, Cesselli, Daniela, Mariuzzi, Laura, Parodi, Pier Camillo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824042/
https://www.ncbi.nlm.nih.gov/pubmed/33396896
http://dx.doi.org/10.3390/cells10010044
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author Caponnetto, Federica
Manini, Ivana
Bulfoni, Michela
Zingaretti, Nicola
Miotti, Giovanni
Di Loreto, Carla
Cesselli, Daniela
Mariuzzi, Laura
Parodi, Pier Camillo
author_facet Caponnetto, Federica
Manini, Ivana
Bulfoni, Michela
Zingaretti, Nicola
Miotti, Giovanni
Di Loreto, Carla
Cesselli, Daniela
Mariuzzi, Laura
Parodi, Pier Camillo
author_sort Caponnetto, Federica
collection PubMed
description Madelung Disease (MD) is a syndrome characterized by the accumulation of aberrant symmetric adipose tissue deposits. The etiology of this disease is yet to be elucidated, even though the presence of comorbidities, either genetic or environmental, has been reported. For this reason, establishing an in vitro model for MD is considered crucial to get insights into its physiopathology. We previously established a protocol for isolation and culture of stem cells from diseased tissues. Therefore, we isolated human adipose-derived stem cells (ASC) from MD patients and compared these cells with those isolated from healthy subjects in terms of surface phenotype, growth kinetic, adipogenic differentiation potential, and molecular alterations. Moreover, we evaluated the ability of the MD-ASC secretome to affect healthy ASC. The results reported a difference in the growth kinetic and surface markers of MD-ASC compared to healthy ASC but not in adipogenic differentiation. The most commonly described mitochondrial mutations were not observed. Still, MD-ASC secretome was able to shift the healthy ASC phenotype to an MD phenotype. This work provides evidence of the possibility of exploiting a patient-based in vitro model for better understanding MD pathophysiology, possibly favoring the development of novel target therapies.
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spelling pubmed-78240422021-01-24 Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization Caponnetto, Federica Manini, Ivana Bulfoni, Michela Zingaretti, Nicola Miotti, Giovanni Di Loreto, Carla Cesselli, Daniela Mariuzzi, Laura Parodi, Pier Camillo Cells Article Madelung Disease (MD) is a syndrome characterized by the accumulation of aberrant symmetric adipose tissue deposits. The etiology of this disease is yet to be elucidated, even though the presence of comorbidities, either genetic or environmental, has been reported. For this reason, establishing an in vitro model for MD is considered crucial to get insights into its physiopathology. We previously established a protocol for isolation and culture of stem cells from diseased tissues. Therefore, we isolated human adipose-derived stem cells (ASC) from MD patients and compared these cells with those isolated from healthy subjects in terms of surface phenotype, growth kinetic, adipogenic differentiation potential, and molecular alterations. Moreover, we evaluated the ability of the MD-ASC secretome to affect healthy ASC. The results reported a difference in the growth kinetic and surface markers of MD-ASC compared to healthy ASC but not in adipogenic differentiation. The most commonly described mitochondrial mutations were not observed. Still, MD-ASC secretome was able to shift the healthy ASC phenotype to an MD phenotype. This work provides evidence of the possibility of exploiting a patient-based in vitro model for better understanding MD pathophysiology, possibly favoring the development of novel target therapies. MDPI 2020-12-30 /pmc/articles/PMC7824042/ /pubmed/33396896 http://dx.doi.org/10.3390/cells10010044 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Caponnetto, Federica
Manini, Ivana
Bulfoni, Michela
Zingaretti, Nicola
Miotti, Giovanni
Di Loreto, Carla
Cesselli, Daniela
Mariuzzi, Laura
Parodi, Pier Camillo
Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
title Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
title_full Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
title_fullStr Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
title_full_unstemmed Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
title_short Human Adipose-Derived Stem Cells in Madelung’s Disease: Morphological and Functional Characterization
title_sort human adipose-derived stem cells in madelung’s disease: morphological and functional characterization
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824042/
https://www.ncbi.nlm.nih.gov/pubmed/33396896
http://dx.doi.org/10.3390/cells10010044
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