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Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells

Background and objectives: Cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathogenesis of atopic diseases such as atopic dermatitis, allergic rhinitis, and asthma. Resveratrol (RSV) exerts various pharmacological effects such as antioxidant, anti-inflammatory, neuroprotecti...

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Autores principales: Moon, Phil-Dong, Han, Na-Ra, Lee, Jin Soo, Jee, Hyun-Woo, Kim, Ji-Hyeon, Kim, Hyung-Min, Jeong, Hyun-Ja
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824250/
https://www.ncbi.nlm.nih.gov/pubmed/33379407
http://dx.doi.org/10.3390/medicina57010021
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author Moon, Phil-Dong
Han, Na-Ra
Lee, Jin Soo
Jee, Hyun-Woo
Kim, Ji-Hyeon
Kim, Hyung-Min
Jeong, Hyun-Ja
author_facet Moon, Phil-Dong
Han, Na-Ra
Lee, Jin Soo
Jee, Hyun-Woo
Kim, Ji-Hyeon
Kim, Hyung-Min
Jeong, Hyun-Ja
author_sort Moon, Phil-Dong
collection PubMed
description Background and objectives: Cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathogenesis of atopic diseases such as atopic dermatitis, allergic rhinitis, and asthma. Resveratrol (RSV) exerts various pharmacological effects such as antioxidant, anti-inflammatory, neuroprotective, and anticancer. Although, it has been verified the beneficial effects of RSV on various subjects, the effect of RSV on thymic stromal lymphopoietin (TSLP) regulation has not been elucidated. Materials and Methods: Here, we examined how RSV regulates TSLP in HMC-1 cells. Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, Western blotting, and calcium assay were performed to evaluate the effect of RSV. Results: TSLP production and mRNA expression were reduced by RSV. RSV down-regulated nuclear factor-κB activation, IκBα phosphorylation as well as activation of receptor-interacting protein2 and caspase-1 in HMC-1 cells. In addition, RSV treatment decreased the up-regulation of intracellular calcium in HMC-1 cells. Conclusions: These results suggest that RSV might be useful for the treatment of atopic diseases through blocking of TSLP.
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spelling pubmed-78242502021-01-24 Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells Moon, Phil-Dong Han, Na-Ra Lee, Jin Soo Jee, Hyun-Woo Kim, Ji-Hyeon Kim, Hyung-Min Jeong, Hyun-Ja Medicina (Kaunas) Article Background and objectives: Cytokine thymic stromal lymphopoietin (TSLP) plays a pivotal role in the pathogenesis of atopic diseases such as atopic dermatitis, allergic rhinitis, and asthma. Resveratrol (RSV) exerts various pharmacological effects such as antioxidant, anti-inflammatory, neuroprotective, and anticancer. Although, it has been verified the beneficial effects of RSV on various subjects, the effect of RSV on thymic stromal lymphopoietin (TSLP) regulation has not been elucidated. Materials and Methods: Here, we examined how RSV regulates TSLP in HMC-1 cells. Enzyme-linked immunosorbent assay, real-time polymerase chain reaction, Western blotting, and calcium assay were performed to evaluate the effect of RSV. Results: TSLP production and mRNA expression were reduced by RSV. RSV down-regulated nuclear factor-κB activation, IκBα phosphorylation as well as activation of receptor-interacting protein2 and caspase-1 in HMC-1 cells. In addition, RSV treatment decreased the up-regulation of intracellular calcium in HMC-1 cells. Conclusions: These results suggest that RSV might be useful for the treatment of atopic diseases through blocking of TSLP. MDPI 2020-12-28 /pmc/articles/PMC7824250/ /pubmed/33379407 http://dx.doi.org/10.3390/medicina57010021 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Moon, Phil-Dong
Han, Na-Ra
Lee, Jin Soo
Jee, Hyun-Woo
Kim, Ji-Hyeon
Kim, Hyung-Min
Jeong, Hyun-Ja
Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
title Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
title_full Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
title_fullStr Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
title_full_unstemmed Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
title_short Effects of Resveratrol on Thymic Stromal Lymphopoietin Expression in Mast Cells
title_sort effects of resveratrol on thymic stromal lymphopoietin expression in mast cells
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824250/
https://www.ncbi.nlm.nih.gov/pubmed/33379407
http://dx.doi.org/10.3390/medicina57010021
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