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Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)

Protein disulfide isomerases (PDI) and PDI-like proteins catalyze the formation and isomerization of protein disulfide bonds in the endoplasmic reticulum and prevent the buildup of misfolded proteins under abiotic stress conditions. In the present study, we conducted the first comprehensive genome-w...

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Autores principales: Wai, Antt Htet, Waseem, Muhammad, Khan, A B M Mahbub Morshed, Nath, Ujjal Kumar, Lee, Do Jin, Kim, Sang Tae, Kim, Chang Kil, Chung, Mi Young
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824348/
https://www.ncbi.nlm.nih.gov/pubmed/33375673
http://dx.doi.org/10.3390/genes12010023
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author Wai, Antt Htet
Waseem, Muhammad
Khan, A B M Mahbub Morshed
Nath, Ujjal Kumar
Lee, Do Jin
Kim, Sang Tae
Kim, Chang Kil
Chung, Mi Young
author_facet Wai, Antt Htet
Waseem, Muhammad
Khan, A B M Mahbub Morshed
Nath, Ujjal Kumar
Lee, Do Jin
Kim, Sang Tae
Kim, Chang Kil
Chung, Mi Young
author_sort Wai, Antt Htet
collection PubMed
description Protein disulfide isomerases (PDI) and PDI-like proteins catalyze the formation and isomerization of protein disulfide bonds in the endoplasmic reticulum and prevent the buildup of misfolded proteins under abiotic stress conditions. In the present study, we conducted the first comprehensive genome-wide exploration of the PDI gene family in tomato (Solanum lycopersicum L.). We identified 19 tomato PDI genes that were unevenly distributed on 8 of the 12 tomato chromosomes, with segmental duplications detected for 3 paralogous gene pairs. Expression profiling of the PDI genes revealed that most of them were differentially expressed across different organs and developmental stages of the fruit. Furthermore, most of the PDI genes were highly induced by heat, salt, and abscisic acid (ABA) treatments, while relatively few of the genes were induced by cold and nutrient and water deficit (NWD) stresses. The predominant expression of SlPDI1-1, SlPDI1-3, SlPDI1-4, SlPDI2-1, SlPDI4-1, and SlPDI5-1 in response to abiotic stress and ABA treatment suggested they play regulatory roles in abiotic stress tolerance in tomato in an ABA-dependent manner. Our results provide new insight into the structure and function of PDI genes and will be helpful for the selection of candidate genes involved in fruit development and abiotic stress tolerance in tomato.
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spelling pubmed-78243482021-01-24 Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.) Wai, Antt Htet Waseem, Muhammad Khan, A B M Mahbub Morshed Nath, Ujjal Kumar Lee, Do Jin Kim, Sang Tae Kim, Chang Kil Chung, Mi Young Genes (Basel) Article Protein disulfide isomerases (PDI) and PDI-like proteins catalyze the formation and isomerization of protein disulfide bonds in the endoplasmic reticulum and prevent the buildup of misfolded proteins under abiotic stress conditions. In the present study, we conducted the first comprehensive genome-wide exploration of the PDI gene family in tomato (Solanum lycopersicum L.). We identified 19 tomato PDI genes that were unevenly distributed on 8 of the 12 tomato chromosomes, with segmental duplications detected for 3 paralogous gene pairs. Expression profiling of the PDI genes revealed that most of them were differentially expressed across different organs and developmental stages of the fruit. Furthermore, most of the PDI genes were highly induced by heat, salt, and abscisic acid (ABA) treatments, while relatively few of the genes were induced by cold and nutrient and water deficit (NWD) stresses. The predominant expression of SlPDI1-1, SlPDI1-3, SlPDI1-4, SlPDI2-1, SlPDI4-1, and SlPDI5-1 in response to abiotic stress and ABA treatment suggested they play regulatory roles in abiotic stress tolerance in tomato in an ABA-dependent manner. Our results provide new insight into the structure and function of PDI genes and will be helpful for the selection of candidate genes involved in fruit development and abiotic stress tolerance in tomato. MDPI 2020-12-25 /pmc/articles/PMC7824348/ /pubmed/33375673 http://dx.doi.org/10.3390/genes12010023 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wai, Antt Htet
Waseem, Muhammad
Khan, A B M Mahbub Morshed
Nath, Ujjal Kumar
Lee, Do Jin
Kim, Sang Tae
Kim, Chang Kil
Chung, Mi Young
Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)
title Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)
title_full Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)
title_fullStr Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)
title_full_unstemmed Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)
title_short Genome-Wide Identification and Expression Profiling of the PDI Gene Family Reveals Their Probable Involvement in Abiotic Stress Tolerance in Tomato (Solanum lycopersicum L.)
title_sort genome-wide identification and expression profiling of the pdi gene family reveals their probable involvement in abiotic stress tolerance in tomato (solanum lycopersicum l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824348/
https://www.ncbi.nlm.nih.gov/pubmed/33375673
http://dx.doi.org/10.3390/genes12010023
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