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Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2

DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci...

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Autores principales: Doniec, Andrzej, Łuczak, Wojciech, Wróbel, Maria, Januła, Miłosz, Ossowski, Andrzej, Grzmil, Paweł, Kupiec, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824413/
https://www.ncbi.nlm.nih.gov/pubmed/33406744
http://dx.doi.org/10.3390/genes12010062
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author Doniec, Andrzej
Łuczak, Wojciech
Wróbel, Maria
Januła, Miłosz
Ossowski, Andrzej
Grzmil, Paweł
Kupiec, Tomasz
author_facet Doniec, Andrzej
Łuczak, Wojciech
Wróbel, Maria
Januła, Miłosz
Ossowski, Andrzej
Grzmil, Paweł
Kupiec, Tomasz
author_sort Doniec, Andrzej
collection PubMed
description DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci between the child and the presumed father may indicate the exclusion of biological parenthood. However, another reason for such differences is genetic mutations, including chromosome aberrations and genome mutations. The presented results relate to genetic analyses carried out on three persons for the purposes of disputed paternity testing. A deviation from inheritance based on Mendel’s Law was found in 7 out of 53 STR-type loci examined. All polymorphic loci that ruled out the paternity of the alleged father were located on chromosome 2. Additional analysis of 32 insertion–deletion markers (DIPplex, Qiagen) and sequencing of 94 polymorphic positions of the single nucleotide polymorphism (SNP) type (Illumina, ForenSeq) did not exclude the defendant’s biological paternity. A sequence analysis of STR alleles and their flanking regions confirmed the hypothesis that the alleles on chromosome 2 of the child may originate only from the mother. The results of the tests did not allow exclusion of the paternity of the alleged father, but are an example of uniparental maternal disomy, which is briefly described in the literature.
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spelling pubmed-78244132021-01-24 Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2 Doniec, Andrzej Łuczak, Wojciech Wróbel, Maria Januła, Miłosz Ossowski, Andrzej Grzmil, Paweł Kupiec, Tomasz Genes (Basel) Article DNA testing in cases of disputed paternity is a routine analysis carried out in genetic laboratories. The purpose of the test is to demonstrate similarities and differences in analyzed genetic markers between the alleged father, mother, and a child. The existence of differences in the examined loci between the child and the presumed father may indicate the exclusion of biological parenthood. However, another reason for such differences is genetic mutations, including chromosome aberrations and genome mutations. The presented results relate to genetic analyses carried out on three persons for the purposes of disputed paternity testing. A deviation from inheritance based on Mendel’s Law was found in 7 out of 53 STR-type loci examined. All polymorphic loci that ruled out the paternity of the alleged father were located on chromosome 2. Additional analysis of 32 insertion–deletion markers (DIPplex, Qiagen) and sequencing of 94 polymorphic positions of the single nucleotide polymorphism (SNP) type (Illumina, ForenSeq) did not exclude the defendant’s biological paternity. A sequence analysis of STR alleles and their flanking regions confirmed the hypothesis that the alleles on chromosome 2 of the child may originate only from the mother. The results of the tests did not allow exclusion of the paternity of the alleged father, but are an example of uniparental maternal disomy, which is briefly described in the literature. MDPI 2021-01-04 /pmc/articles/PMC7824413/ /pubmed/33406744 http://dx.doi.org/10.3390/genes12010062 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Doniec, Andrzej
Łuczak, Wojciech
Wróbel, Maria
Januła, Miłosz
Ossowski, Andrzej
Grzmil, Paweł
Kupiec, Tomasz
Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
title Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
title_full Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
title_fullStr Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
title_full_unstemmed Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
title_short Confirmation of Paternity despite Three Genetic Incompatibilities at Chromosome 2
title_sort confirmation of paternity despite three genetic incompatibilities at chromosome 2
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824413/
https://www.ncbi.nlm.nih.gov/pubmed/33406744
http://dx.doi.org/10.3390/genes12010062
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