PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity

P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regula...

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Autores principales: He, Sijia, Waheed, Abdul A., Hetrick, Brian, Dabbagh, Deemah, Akhrymuk, Ivan V., Kehn-Hall, Kylene, Freed, Eric O., Wu, Yuntao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Brief Report
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824426/
https://www.ncbi.nlm.nih.gov/pubmed/33396594
http://dx.doi.org/10.3390/v13010046
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author He, Sijia
Waheed, Abdul A.
Hetrick, Brian
Dabbagh, Deemah
Akhrymuk, Ivan V.
Kehn-Hall, Kylene
Freed, Eric O.
Wu, Yuntao
author_facet He, Sijia
Waheed, Abdul A.
Hetrick, Brian
Dabbagh, Deemah
Akhrymuk, Ivan V.
Kehn-Hall, Kylene
Freed, Eric O.
Wu, Yuntao
author_sort He, Sijia
collection PubMed
description P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1(+) cells
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spelling pubmed-78244262021-01-24 PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity He, Sijia Waheed, Abdul A. Hetrick, Brian Dabbagh, Deemah Akhrymuk, Ivan V. Kehn-Hall, Kylene Freed, Eric O. Wu, Yuntao Viruses Brief Report P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1(+) cells MDPI 2020-12-30 /pmc/articles/PMC7824426/ /pubmed/33396594 http://dx.doi.org/10.3390/v13010046 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Brief Report
He, Sijia
Waheed, Abdul A.
Hetrick, Brian
Dabbagh, Deemah
Akhrymuk, Ivan V.
Kehn-Hall, Kylene
Freed, Eric O.
Wu, Yuntao
PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
title PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
title_full PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
title_fullStr PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
title_full_unstemmed PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
title_short PSGL-1 Inhibits the Incorporation of SARS-CoV and SARS-CoV-2 Spike Glycoproteins into Pseudovirions and Impairs Pseudovirus Attachment and Infectivity
title_sort psgl-1 inhibits the incorporation of sars-cov and sars-cov-2 spike glycoproteins into pseudovirions and impairs pseudovirus attachment and infectivity
topic Brief Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824426/
https://www.ncbi.nlm.nih.gov/pubmed/33396594
http://dx.doi.org/10.3390/v13010046
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