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The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels....
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824728/ https://www.ncbi.nlm.nih.gov/pubmed/33379305 http://dx.doi.org/10.3390/microorganisms9010060 |
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author | Papaianni, Marina Ricciardelli, Annarita Casillo, Angela Corsaro, Maria M. Borbone, Fabio Della Ventura, Bartolomeo Velotta, Raffaele Fulgione, Andrea Woo, Sheridan L. Tutino, Maria L. Parrilli, Ermenegilda Capparelli, Rosanna |
author_facet | Papaianni, Marina Ricciardelli, Annarita Casillo, Angela Corsaro, Maria M. Borbone, Fabio Della Ventura, Bartolomeo Velotta, Raffaele Fulgione, Andrea Woo, Sheridan L. Tutino, Maria L. Parrilli, Ermenegilda Capparelli, Rosanna |
author_sort | Papaianni, Marina |
collection | PubMed |
description | Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels. The discovery of novel approaches to crop disease management is urgent and a possible treatment could be aimed at the eradication of biofilm, although anti-biofilm approaches in agricultural microbiology are still rare. Considering the multifactorial nature of biofilm, an effective approach against Xanthomonas campestris implies the use of a multi-targeted or combinatorial strategy. In this paper, an anti-biofilm strategy based on the use of fatty acids and the bacteriophage (Xccφ1)-hydroxyapatite complex was optimized against Xanthomonas campestris mature biofilm. The synergic action of these elements was demonstrated and the efficient removal of Xanthomonas campestris mature biofilm was also proven in a flow cell system, making the proposed approach an effective solution to enhance plant survival in Xanthomonas campestris infections. Moreover, the molecular mechanisms responsible for the efficacy of the proposed treatment were explored. |
format | Online Article Text |
id | pubmed-7824728 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78247282021-01-24 The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm Papaianni, Marina Ricciardelli, Annarita Casillo, Angela Corsaro, Maria M. Borbone, Fabio Della Ventura, Bartolomeo Velotta, Raffaele Fulgione, Andrea Woo, Sheridan L. Tutino, Maria L. Parrilli, Ermenegilda Capparelli, Rosanna Microorganisms Article Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels. The discovery of novel approaches to crop disease management is urgent and a possible treatment could be aimed at the eradication of biofilm, although anti-biofilm approaches in agricultural microbiology are still rare. Considering the multifactorial nature of biofilm, an effective approach against Xanthomonas campestris implies the use of a multi-targeted or combinatorial strategy. In this paper, an anti-biofilm strategy based on the use of fatty acids and the bacteriophage (Xccφ1)-hydroxyapatite complex was optimized against Xanthomonas campestris mature biofilm. The synergic action of these elements was demonstrated and the efficient removal of Xanthomonas campestris mature biofilm was also proven in a flow cell system, making the proposed approach an effective solution to enhance plant survival in Xanthomonas campestris infections. Moreover, the molecular mechanisms responsible for the efficacy of the proposed treatment were explored. MDPI 2020-12-28 /pmc/articles/PMC7824728/ /pubmed/33379305 http://dx.doi.org/10.3390/microorganisms9010060 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Papaianni, Marina Ricciardelli, Annarita Casillo, Angela Corsaro, Maria M. Borbone, Fabio Della Ventura, Bartolomeo Velotta, Raffaele Fulgione, Andrea Woo, Sheridan L. Tutino, Maria L. Parrilli, Ermenegilda Capparelli, Rosanna The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm |
title | The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm |
title_full | The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm |
title_fullStr | The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm |
title_full_unstemmed | The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm |
title_short | The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm |
title_sort | union is strength: the synergic action of long fatty acids and a bacteriophage against xanthomonas campestris biofilm |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824728/ https://www.ncbi.nlm.nih.gov/pubmed/33379305 http://dx.doi.org/10.3390/microorganisms9010060 |
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