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The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm

Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels....

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Autores principales: Papaianni, Marina, Ricciardelli, Annarita, Casillo, Angela, Corsaro, Maria M., Borbone, Fabio, Della Ventura, Bartolomeo, Velotta, Raffaele, Fulgione, Andrea, Woo, Sheridan L., Tutino, Maria L., Parrilli, Ermenegilda, Capparelli, Rosanna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824728/
https://www.ncbi.nlm.nih.gov/pubmed/33379305
http://dx.doi.org/10.3390/microorganisms9010060
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author Papaianni, Marina
Ricciardelli, Annarita
Casillo, Angela
Corsaro, Maria M.
Borbone, Fabio
Della Ventura, Bartolomeo
Velotta, Raffaele
Fulgione, Andrea
Woo, Sheridan L.
Tutino, Maria L.
Parrilli, Ermenegilda
Capparelli, Rosanna
author_facet Papaianni, Marina
Ricciardelli, Annarita
Casillo, Angela
Corsaro, Maria M.
Borbone, Fabio
Della Ventura, Bartolomeo
Velotta, Raffaele
Fulgione, Andrea
Woo, Sheridan L.
Tutino, Maria L.
Parrilli, Ermenegilda
Capparelli, Rosanna
author_sort Papaianni, Marina
collection PubMed
description Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels. The discovery of novel approaches to crop disease management is urgent and a possible treatment could be aimed at the eradication of biofilm, although anti-biofilm approaches in agricultural microbiology are still rare. Considering the multifactorial nature of biofilm, an effective approach against Xanthomonas campestris implies the use of a multi-targeted or combinatorial strategy. In this paper, an anti-biofilm strategy based on the use of fatty acids and the bacteriophage (Xccφ1)-hydroxyapatite complex was optimized against Xanthomonas campestris mature biofilm. The synergic action of these elements was demonstrated and the efficient removal of Xanthomonas campestris mature biofilm was also proven in a flow cell system, making the proposed approach an effective solution to enhance plant survival in Xanthomonas campestris infections. Moreover, the molecular mechanisms responsible for the efficacy of the proposed treatment were explored.
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spelling pubmed-78247282021-01-24 The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm Papaianni, Marina Ricciardelli, Annarita Casillo, Angela Corsaro, Maria M. Borbone, Fabio Della Ventura, Bartolomeo Velotta, Raffaele Fulgione, Andrea Woo, Sheridan L. Tutino, Maria L. Parrilli, Ermenegilda Capparelli, Rosanna Microorganisms Article Xanthomonas campestris pv. campestris is known as the causative agent of black rot disease, which attacks mainly crucifers, severely lowering their global productivity. One of the main virulence factors of this pathogen is its capability to penetrate and form biofilm structures in the xylem vessels. The discovery of novel approaches to crop disease management is urgent and a possible treatment could be aimed at the eradication of biofilm, although anti-biofilm approaches in agricultural microbiology are still rare. Considering the multifactorial nature of biofilm, an effective approach against Xanthomonas campestris implies the use of a multi-targeted or combinatorial strategy. In this paper, an anti-biofilm strategy based on the use of fatty acids and the bacteriophage (Xccφ1)-hydroxyapatite complex was optimized against Xanthomonas campestris mature biofilm. The synergic action of these elements was demonstrated and the efficient removal of Xanthomonas campestris mature biofilm was also proven in a flow cell system, making the proposed approach an effective solution to enhance plant survival in Xanthomonas campestris infections. Moreover, the molecular mechanisms responsible for the efficacy of the proposed treatment were explored. MDPI 2020-12-28 /pmc/articles/PMC7824728/ /pubmed/33379305 http://dx.doi.org/10.3390/microorganisms9010060 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Papaianni, Marina
Ricciardelli, Annarita
Casillo, Angela
Corsaro, Maria M.
Borbone, Fabio
Della Ventura, Bartolomeo
Velotta, Raffaele
Fulgione, Andrea
Woo, Sheridan L.
Tutino, Maria L.
Parrilli, Ermenegilda
Capparelli, Rosanna
The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
title The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
title_full The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
title_fullStr The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
title_full_unstemmed The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
title_short The Union Is Strength: The Synergic Action of Long Fatty Acids and a Bacteriophage against Xanthomonas campestris Biofilm
title_sort union is strength: the synergic action of long fatty acids and a bacteriophage against xanthomonas campestris biofilm
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824728/
https://www.ncbi.nlm.nih.gov/pubmed/33379305
http://dx.doi.org/10.3390/microorganisms9010060
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