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Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli....
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824746/ https://www.ncbi.nlm.nih.gov/pubmed/33466290 http://dx.doi.org/10.3390/microorganisms9010101 |
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author | Al Rugaie, Osamah Jabir, Majid Kadhim, Rua Karsh, Esraa Sulaiman, Ghassan M. Mohammed, Salman A. A. Khan, Riaz A. Mohammed, Hamdoon A. |
author_facet | Al Rugaie, Osamah Jabir, Majid Kadhim, Rua Karsh, Esraa Sulaiman, Ghassan M. Mohammed, Salman A. A. Khan, Riaz A. Mohammed, Hamdoon A. |
author_sort | Al Rugaie, Osamah |
collection | PubMed |
description | Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation (p < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, p < 0.0001) through the NADPH oxidase 2 (NOX2, p < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis (p < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, p < 0.0001). The current study demonstrated that GNPs’ and GOFs’ bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains. |
format | Online Article Text |
id | pubmed-7824746 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78247462021-01-24 Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity Al Rugaie, Osamah Jabir, Majid Kadhim, Rua Karsh, Esraa Sulaiman, Ghassan M. Mohammed, Salman A. A. Khan, Riaz A. Mohammed, Hamdoon A. Microorganisms Article Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation (p < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, p < 0.0001) through the NADPH oxidase 2 (NOX2, p < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis (p < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, p < 0.0001). The current study demonstrated that GNPs’ and GOFs’ bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains. MDPI 2021-01-05 /pmc/articles/PMC7824746/ /pubmed/33466290 http://dx.doi.org/10.3390/microorganisms9010101 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Al Rugaie, Osamah Jabir, Majid Kadhim, Rua Karsh, Esraa Sulaiman, Ghassan M. Mohammed, Salman A. A. Khan, Riaz A. Mohammed, Hamdoon A. Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity |
title | Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity |
title_full | Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity |
title_fullStr | Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity |
title_full_unstemmed | Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity |
title_short | Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity |
title_sort | gold nanoparticles and graphene oxide flakes synergistic partaking in cytosolic bactericidal augmentation: role of ros and nox2 activity |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824746/ https://www.ncbi.nlm.nih.gov/pubmed/33466290 http://dx.doi.org/10.3390/microorganisms9010101 |
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