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Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity

Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli....

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Autores principales: Al Rugaie, Osamah, Jabir, Majid, Kadhim, Rua, Karsh, Esraa, Sulaiman, Ghassan M., Mohammed, Salman A. A., Khan, Riaz A., Mohammed, Hamdoon A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824746/
https://www.ncbi.nlm.nih.gov/pubmed/33466290
http://dx.doi.org/10.3390/microorganisms9010101
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author Al Rugaie, Osamah
Jabir, Majid
Kadhim, Rua
Karsh, Esraa
Sulaiman, Ghassan M.
Mohammed, Salman A. A.
Khan, Riaz A.
Mohammed, Hamdoon A.
author_facet Al Rugaie, Osamah
Jabir, Majid
Kadhim, Rua
Karsh, Esraa
Sulaiman, Ghassan M.
Mohammed, Salman A. A.
Khan, Riaz A.
Mohammed, Hamdoon A.
author_sort Al Rugaie, Osamah
collection PubMed
description Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation (p < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, p < 0.0001) through the NADPH oxidase 2 (NOX2, p < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis (p < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, p < 0.0001). The current study demonstrated that GNPs’ and GOFs’ bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains.
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spelling pubmed-78247462021-01-24 Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity Al Rugaie, Osamah Jabir, Majid Kadhim, Rua Karsh, Esraa Sulaiman, Ghassan M. Mohammed, Salman A. A. Khan, Riaz A. Mohammed, Hamdoon A. Microorganisms Article Gold nanoparticles (GNPs) and graphene oxide flakes (GOFs) exerted significantly (p < 0.0001) supportive roles on the phagocytosis bioactivity of the immune cells of phagocytic nature against the Gram-positive and Gram-negative human pathogenic bacteria Staphylococcus aureus and Escherichia coli. Under experimental conditions, upon bacterial exposure, the combined GNPs and GOFs induced significant clearance of bacteria through phagosome maturation (p < 0.0001) from time-points of 6 to 30 min and production of reactive oxygen species (ROS, p < 0.0001) through the NADPH oxidase 2 (NOX2, p < 0.0001)-based feedback mechanism. The effects of the combined presence of GNPs and GOFs on phagocytosis (p < 0.0001) suggested a synergistic action underway, also achieved through elevated signal transduction activity in the bone-marrow-derived macrophages (BMDM, p < 0.0001). The current study demonstrated that GNPs’ and GOFs’ bactericidal assisting potentials could be considered an effective and alternative strategy for treating infections from both positive and negative bacterial strains. MDPI 2021-01-05 /pmc/articles/PMC7824746/ /pubmed/33466290 http://dx.doi.org/10.3390/microorganisms9010101 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Al Rugaie, Osamah
Jabir, Majid
Kadhim, Rua
Karsh, Esraa
Sulaiman, Ghassan M.
Mohammed, Salman A. A.
Khan, Riaz A.
Mohammed, Hamdoon A.
Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
title Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
title_full Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
title_fullStr Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
title_full_unstemmed Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
title_short Gold Nanoparticles and Graphene Oxide Flakes Synergistic Partaking in Cytosolic Bactericidal Augmentation: Role of ROS and NOX2 Activity
title_sort gold nanoparticles and graphene oxide flakes synergistic partaking in cytosolic bactericidal augmentation: role of ros and nox2 activity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824746/
https://www.ncbi.nlm.nih.gov/pubmed/33466290
http://dx.doi.org/10.3390/microorganisms9010101
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