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A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization
Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824798/ https://www.ncbi.nlm.nih.gov/pubmed/33466223 http://dx.doi.org/10.3390/toxins13010030 |
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author | Teixeira-Cruz, Jhonatha Mota Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Jr., Rui Seabra Barraviera, Benedito Quintas, Luis Eduardo M. Melo, Paulo A. |
author_facet | Teixeira-Cruz, Jhonatha Mota Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Jr., Rui Seabra Barraviera, Benedito Quintas, Luis Eduardo M. Melo, Paulo A. |
author_sort | Teixeira-Cruz, Jhonatha Mota |
collection | PubMed |
description | Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab’)2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25–0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1–1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks. |
format | Online Article Text |
id | pubmed-7824798 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78247982021-01-24 A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization Teixeira-Cruz, Jhonatha Mota Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Jr., Rui Seabra Barraviera, Benedito Quintas, Luis Eduardo M. Melo, Paulo A. Toxins (Basel) Article Massive, Africanized honeybee attacks have increased in Brazil over the years. Humans and animals present local and systemic effects after envenomation, and there is no specific treatment for this potentially lethal event. This study evaluated the ability of a new Apilic antivenom, which is composed of F(ab’)2 fraction of specific immunoglobulins in heterologous and hyperimmune equine serum, to neutralize A. mellifera venom and melittin, in vitro and in vivo, in mice. Animal experiments were performed in according with local ethics committee license (UFRJ protocol no. DFBCICB072-04/16). Venom dose-dependent lethality was diminished with 0.25–0.5 μL of intravenous Apilic antivenom/μg honeybee venom. In vivo injection of 0.1–1 μg/g bee venom induced myotoxicity, hemoconcentration, paw edema, and increase of vascular permeability which were antagonized by Apilic antivenom. Cytotoxicity, assessed in renal LLC-PK1 cells and challenged with 10 μg/mL honeybee venom or melittin, was neutralized by preincubation with Apilic antivenom, as well the hemolytic activity. Apilic antivenom inhibited phospholipase and hyaluronidase enzymatic activities. In flow cytometry experiments, Apilic antivenom neutralized reduction of cell viability due to necrosis by honeybee venom or melittin. These results showed that this antivenom is effective inhibitor of honeybee venom actions. Thus, this next generation of Apilic antivenom emerges as a new promising immunobiological product for the treatment of massive, Africanized honeybee attacks. MDPI 2021-01-05 /pmc/articles/PMC7824798/ /pubmed/33466223 http://dx.doi.org/10.3390/toxins13010030 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Teixeira-Cruz, Jhonatha Mota Strauch, Marcelo Abrahão Monteiro-Machado, Marcos Tavares-Henriques, Matheus Silva de Moraes, João Alfredo Ribeiro da Cunha, Luís Eduardo Ferreira, Jr., Rui Seabra Barraviera, Benedito Quintas, Luis Eduardo M. Melo, Paulo A. A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title | A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_full | A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_fullStr | A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_full_unstemmed | A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_short | A Novel Apilic Antivenom to Treat Massive, Africanized Honeybee Attacks: A Preclinical Study from the Lethality to Some Biochemical and Pharmacological Activities Neutralization |
title_sort | novel apilic antivenom to treat massive, africanized honeybee attacks: a preclinical study from the lethality to some biochemical and pharmacological activities neutralization |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824798/ https://www.ncbi.nlm.nih.gov/pubmed/33466223 http://dx.doi.org/10.3390/toxins13010030 |
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