Cargando…

Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes

BACKGROUND: This is an exploratory study using multimodal magnetic resonance imaging (MRI) to interrogate the brain of rats with type 2 diabetes (T2DM) as compared to controls. It was hypothesized there would be changes in brain structure and function that reflected the human disorder, thus providin...

Descripción completa

Detalles Bibliográficos
Autores principales: Lawson, Christopher M., Rentrup, Kilian F. G., Cai, Xuezhu, Kulkarni, Praveen P., Ferris, Craig F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824967/
https://www.ncbi.nlm.nih.gov/pubmed/33532703
http://dx.doi.org/10.1002/ame2.12140
_version_ 1783640205343850496
author Lawson, Christopher M.
Rentrup, Kilian F. G.
Cai, Xuezhu
Kulkarni, Praveen P.
Ferris, Craig F.
author_facet Lawson, Christopher M.
Rentrup, Kilian F. G.
Cai, Xuezhu
Kulkarni, Praveen P.
Ferris, Craig F.
author_sort Lawson, Christopher M.
collection PubMed
description BACKGROUND: This is an exploratory study using multimodal magnetic resonance imaging (MRI) to interrogate the brain of rats with type 2 diabetes (T2DM) as compared to controls. It was hypothesized there would be changes in brain structure and function that reflected the human disorder, thus providing a model system by which to follow disease progression with noninvasive MRI. METHODS: The transgenic BBZDR/Wor rat, an animal model of T2MD, and age‐matched controls were studied for changes in brain structure using voxel‐based morphometry, alteration in white and gray matter microarchitecture using diffusion weighted imaging with indices of anisotropy, and functional coupling using resting‐state BOLD functional connectivity. Images from each modality were registered to, and analyzed, using a 3D MRI rat atlas providing site‐specific data on over 168 different brain areas. RESULTS: There was an overall reduction in brain volume focused primarily on the somatosensory cortex, cerebellum, and white matter tracts. The putative changes in white and gray matter microarchitecture were pervasive affecting much of the brain and not localized to any region. There was a general increase in connectivity in T2DM rats as compared to controls. The cerebellum presented with strong functional coupling to pons and brainstem in T2DM rats but negative connectivity to hippocampus. CONCLUSION: The neuroradiological measures collected in BBBKZ/Wor rats using multimodal imaging methods did not reflect those reported for T2DB patients in the clinic. The data would suggest the BBBKZ/Wor rat is not an appropriate imaging model for T2DM.
format Online
Article
Text
id pubmed-7824967
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-78249672021-02-01 Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes Lawson, Christopher M. Rentrup, Kilian F. G. Cai, Xuezhu Kulkarni, Praveen P. Ferris, Craig F. Animal Model Exp Med Original Articles BACKGROUND: This is an exploratory study using multimodal magnetic resonance imaging (MRI) to interrogate the brain of rats with type 2 diabetes (T2DM) as compared to controls. It was hypothesized there would be changes in brain structure and function that reflected the human disorder, thus providing a model system by which to follow disease progression with noninvasive MRI. METHODS: The transgenic BBZDR/Wor rat, an animal model of T2MD, and age‐matched controls were studied for changes in brain structure using voxel‐based morphometry, alteration in white and gray matter microarchitecture using diffusion weighted imaging with indices of anisotropy, and functional coupling using resting‐state BOLD functional connectivity. Images from each modality were registered to, and analyzed, using a 3D MRI rat atlas providing site‐specific data on over 168 different brain areas. RESULTS: There was an overall reduction in brain volume focused primarily on the somatosensory cortex, cerebellum, and white matter tracts. The putative changes in white and gray matter microarchitecture were pervasive affecting much of the brain and not localized to any region. There was a general increase in connectivity in T2DM rats as compared to controls. The cerebellum presented with strong functional coupling to pons and brainstem in T2DM rats but negative connectivity to hippocampus. CONCLUSION: The neuroradiological measures collected in BBBKZ/Wor rats using multimodal imaging methods did not reflect those reported for T2DB patients in the clinic. The data would suggest the BBBKZ/Wor rat is not an appropriate imaging model for T2DM. John Wiley and Sons Inc. 2020-12-29 /pmc/articles/PMC7824967/ /pubmed/33532703 http://dx.doi.org/10.1002/ame2.12140 Text en © 2020 The Authors. Animal Models and Experimental Medicine published by John Wiley & Sons Australia, Ltd on behalf of The Chinese Association for Laboratory Animal Sciences This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Lawson, Christopher M.
Rentrup, Kilian F. G.
Cai, Xuezhu
Kulkarni, Praveen P.
Ferris, Craig F.
Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes
title Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes
title_full Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes
title_fullStr Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes
title_full_unstemmed Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes
title_short Using multimodal MRI to investigate alterations in brain structure and function in the BBZDR/Wor rat model of type 2 diabetes
title_sort using multimodal mri to investigate alterations in brain structure and function in the bbzdr/wor rat model of type 2 diabetes
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7824967/
https://www.ncbi.nlm.nih.gov/pubmed/33532703
http://dx.doi.org/10.1002/ame2.12140
work_keys_str_mv AT lawsonchristopherm usingmultimodalmritoinvestigatealterationsinbrainstructureandfunctioninthebbzdrworratmodeloftype2diabetes
AT rentrupkilianfg usingmultimodalmritoinvestigatealterationsinbrainstructureandfunctioninthebbzdrworratmodeloftype2diabetes
AT caixuezhu usingmultimodalmritoinvestigatealterationsinbrainstructureandfunctioninthebbzdrworratmodeloftype2diabetes
AT kulkarnipraveenp usingmultimodalmritoinvestigatealterationsinbrainstructureandfunctioninthebbzdrworratmodeloftype2diabetes
AT ferriscraigf usingmultimodalmritoinvestigatealterationsinbrainstructureandfunctioninthebbzdrworratmodeloftype2diabetes