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The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors

The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition. Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providi...

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Autores principales: Váradi, Csaba, Hajdu, Viktória, Farkas, Flóra, Gilányi, Ibolya, Oláh, Csaba, Viskolcz, Béla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825111/
https://www.ncbi.nlm.nih.gov/pubmed/33418875
http://dx.doi.org/10.3390/life11010029
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author Váradi, Csaba
Hajdu, Viktória
Farkas, Flóra
Gilányi, Ibolya
Oláh, Csaba
Viskolcz, Béla
author_facet Váradi, Csaba
Hajdu, Viktória
Farkas, Flóra
Gilányi, Ibolya
Oláh, Csaba
Viskolcz, Béla
author_sort Váradi, Csaba
collection PubMed
description The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition. Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection. In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors. PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis. The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions. Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern. Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated–fucosylated structures were found to be the most significant changes. Our results demonstrate that serum glycosylation monitoring could improve the detection of malignancy.
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spelling pubmed-78251112021-01-24 The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors Váradi, Csaba Hajdu, Viktória Farkas, Flóra Gilányi, Ibolya Oláh, Csaba Viskolcz, Béla Life (Basel) Communication The identification of patients with different brain tumors is solely built on imaging diagnostics, indicating the need for novel methods to facilitate disease recognition. Glycosylation is a chemical modification of proteins, reportedly altered in several inflammatory and malignant diseases, providing a potential alternative route for disease detection. In this paper, we report the quantitative analysis of serum N-glycosylation of patients diagnosed with primary and metastatic brain tumors. PNGase-F-digested and procainamide-labeled serum glycans were purified by magnetic nanoparticles, followed by quantitative liquid chromatographic analysis. The glycan structures were identified by the combination of single quad mass spectrometric detection and exoglycosidase digestions. Linear discriminant analysis provided a clear separation of different disease groups and healthy controls based on their N-glycome pattern. Altered distribution of biantennary neutral, sialylated but nonfucosylated, and sialylated–fucosylated structures were found to be the most significant changes. Our results demonstrate that serum glycosylation monitoring could improve the detection of malignancy. MDPI 2021-01-06 /pmc/articles/PMC7825111/ /pubmed/33418875 http://dx.doi.org/10.3390/life11010029 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Váradi, Csaba
Hajdu, Viktória
Farkas, Flóra
Gilányi, Ibolya
Oláh, Csaba
Viskolcz, Béla
The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors
title The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors
title_full The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors
title_fullStr The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors
title_full_unstemmed The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors
title_short The Analysis of Human Serum N-Glycosylation in Patients with Primary and Metastatic Brain Tumors
title_sort analysis of human serum n-glycosylation in patients with primary and metastatic brain tumors
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825111/
https://www.ncbi.nlm.nih.gov/pubmed/33418875
http://dx.doi.org/10.3390/life11010029
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