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Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters

Rapid diagnostic tests (RDTs) are often used for the detection of anti-human immunodeficiency virus (HIV) antibodies in remote locations in low- and middle-income countries (LMIC) with low or limited access to central laboratories. The typical format of an RDT is a lateral flow assay (LFA) with visu...

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Autores principales: Martiskainen, Iida, Juntunen, Etvi, Salminen, Teppo, Vuorenpää, Karoliina, Bayoumy, Sherif, Vuorinen, Tytti, Khanna, Navin, Pettersson, Kim, Batra, Gaurav, Talha, Sheikh M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825344/
https://www.ncbi.nlm.nih.gov/pubmed/33418986
http://dx.doi.org/10.3390/s21020330
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author Martiskainen, Iida
Juntunen, Etvi
Salminen, Teppo
Vuorenpää, Karoliina
Bayoumy, Sherif
Vuorinen, Tytti
Khanna, Navin
Pettersson, Kim
Batra, Gaurav
Talha, Sheikh M.
author_facet Martiskainen, Iida
Juntunen, Etvi
Salminen, Teppo
Vuorenpää, Karoliina
Bayoumy, Sherif
Vuorinen, Tytti
Khanna, Navin
Pettersson, Kim
Batra, Gaurav
Talha, Sheikh M.
author_sort Martiskainen, Iida
collection PubMed
description Rapid diagnostic tests (RDTs) are often used for the detection of anti-human immunodeficiency virus (HIV) antibodies in remote locations in low- and middle-income countries (LMIC) with low or limited access to central laboratories. The typical format of an RDT is a lateral flow assay (LFA) with visual interpretation prone to subjectivity. This risk of misinterpretation can be overcome with luminescent upconverting nanoparticle reporters (UCNPs) measured with a miniaturized easy-to-use reader instrument. An LFA with UCNPs for anti-HIV-1/2 antibodies was developed and the assay performance was evaluated extensively with challenging patient sample panels. Sensitivity (n = 145) of the UCNP-LFA was 96.6% (95% CI: 92.1–98.8%) and specificity (n = 309) was 98.7% (95% CI: 96.7–99.7%). Another set of samples (n = 200) was used for a comparison between the UCNP-LFA and a conventional visual RDT. In this comparison, the sensitivities for HIV-1 were 96.4% (95% CI: 89.8–99.3%) and 97.6% (95% CI: 91.6–99.7%), for the UCNP-LFA and conventional RDT, respectively. The specificity was 100% (95% CI: 96.4–100%) for both assays. The developed UCNP-LFA demonstrates the applicability of UCNPs for the detection of anti-HIV antibodies. The signal measurement is done by a reader instrument, which may facilitate automated result interpretation, archiving and transfer of data from de-centralized locations.
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spelling pubmed-78253442021-01-24 Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters Martiskainen, Iida Juntunen, Etvi Salminen, Teppo Vuorenpää, Karoliina Bayoumy, Sherif Vuorinen, Tytti Khanna, Navin Pettersson, Kim Batra, Gaurav Talha, Sheikh M. Sensors (Basel) Article Rapid diagnostic tests (RDTs) are often used for the detection of anti-human immunodeficiency virus (HIV) antibodies in remote locations in low- and middle-income countries (LMIC) with low or limited access to central laboratories. The typical format of an RDT is a lateral flow assay (LFA) with visual interpretation prone to subjectivity. This risk of misinterpretation can be overcome with luminescent upconverting nanoparticle reporters (UCNPs) measured with a miniaturized easy-to-use reader instrument. An LFA with UCNPs for anti-HIV-1/2 antibodies was developed and the assay performance was evaluated extensively with challenging patient sample panels. Sensitivity (n = 145) of the UCNP-LFA was 96.6% (95% CI: 92.1–98.8%) and specificity (n = 309) was 98.7% (95% CI: 96.7–99.7%). Another set of samples (n = 200) was used for a comparison between the UCNP-LFA and a conventional visual RDT. In this comparison, the sensitivities for HIV-1 were 96.4% (95% CI: 89.8–99.3%) and 97.6% (95% CI: 91.6–99.7%), for the UCNP-LFA and conventional RDT, respectively. The specificity was 100% (95% CI: 96.4–100%) for both assays. The developed UCNP-LFA demonstrates the applicability of UCNPs for the detection of anti-HIV antibodies. The signal measurement is done by a reader instrument, which may facilitate automated result interpretation, archiving and transfer of data from de-centralized locations. MDPI 2021-01-06 /pmc/articles/PMC7825344/ /pubmed/33418986 http://dx.doi.org/10.3390/s21020330 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Martiskainen, Iida
Juntunen, Etvi
Salminen, Teppo
Vuorenpää, Karoliina
Bayoumy, Sherif
Vuorinen, Tytti
Khanna, Navin
Pettersson, Kim
Batra, Gaurav
Talha, Sheikh M.
Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters
title Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters
title_full Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters
title_fullStr Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters
title_full_unstemmed Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters
title_short Double-Antigen Lateral Flow Immunoassay for the Detection of Anti-HIV-1 and -2 Antibodies Using Upconverting Nanoparticle Reporters
title_sort double-antigen lateral flow immunoassay for the detection of anti-hiv-1 and -2 antibodies using upconverting nanoparticle reporters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825344/
https://www.ncbi.nlm.nih.gov/pubmed/33418986
http://dx.doi.org/10.3390/s21020330
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