Polymer-Coated Magnetite Nanoparticles for Protein Immobilization

Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials de...

Descripción completa

Detalles Bibliográficos
Autores principales: Mylkie, Kinga, Nowak, Pawel, Rybczynski, Patryk, Ziegler-Borowska, Marta
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825442/
https://www.ncbi.nlm.nih.gov/pubmed/33419055
http://dx.doi.org/10.3390/ma14020248
_version_ 1783640309740077056
author Mylkie, Kinga
Nowak, Pawel
Rybczynski, Patryk
Ziegler-Borowska, Marta
author_facet Mylkie, Kinga
Nowak, Pawel
Rybczynski, Patryk
Ziegler-Borowska, Marta
author_sort Mylkie, Kinga
collection PubMed
description Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials depends on their size, shape, core, and shell nature. Some of the most commonly used MNPs are those based on a magnetite core. On the other hand, synthetic biopolymers are used as a protective surface coating for these nanoparticles. This review describes the advances in the field of polymer-coated MNPs for protein immobilization over the past decade. General methods of MNP preparation and protein immobilization are presented. The most extensive section of this article discusses the latest work on the use of polymer-coated MNPs for the physical and chemical immobilization of three types of proteins: enzymes, antibodies, and serum proteins. Where possible, the effectiveness of the immobilization and the activity and use of the immobilized protein are reported. Finally, the information available in the peer-reviewed literature and the application perspectives for the MNP-immobilized protein systems are summarized as well.
format Online
Article
Text
id pubmed-7825442
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78254422021-01-24 Polymer-Coated Magnetite Nanoparticles for Protein Immobilization Mylkie, Kinga Nowak, Pawel Rybczynski, Patryk Ziegler-Borowska, Marta Materials (Basel) Review Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials depends on their size, shape, core, and shell nature. Some of the most commonly used MNPs are those based on a magnetite core. On the other hand, synthetic biopolymers are used as a protective surface coating for these nanoparticles. This review describes the advances in the field of polymer-coated MNPs for protein immobilization over the past decade. General methods of MNP preparation and protein immobilization are presented. The most extensive section of this article discusses the latest work on the use of polymer-coated MNPs for the physical and chemical immobilization of three types of proteins: enzymes, antibodies, and serum proteins. Where possible, the effectiveness of the immobilization and the activity and use of the immobilized protein are reported. Finally, the information available in the peer-reviewed literature and the application perspectives for the MNP-immobilized protein systems are summarized as well. MDPI 2021-01-06 /pmc/articles/PMC7825442/ /pubmed/33419055 http://dx.doi.org/10.3390/ma14020248 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Review
Mylkie, Kinga
Nowak, Pawel
Rybczynski, Patryk
Ziegler-Borowska, Marta
Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
title Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
title_full Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
title_fullStr Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
title_full_unstemmed Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
title_short Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
title_sort polymer-coated magnetite nanoparticles for protein immobilization
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825442/
https://www.ncbi.nlm.nih.gov/pubmed/33419055
http://dx.doi.org/10.3390/ma14020248
work_keys_str_mv AT mylkiekinga polymercoatedmagnetitenanoparticlesforproteinimmobilization
AT nowakpawel polymercoatedmagnetitenanoparticlesforproteinimmobilization
AT rybczynskipatryk polymercoatedmagnetitenanoparticlesforproteinimmobilization
AT zieglerborowskamarta polymercoatedmagnetitenanoparticlesforproteinimmobilization