Polymer-Coated Magnetite Nanoparticles for Protein Immobilization
Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials de...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825442/ https://www.ncbi.nlm.nih.gov/pubmed/33419055 http://dx.doi.org/10.3390/ma14020248 |
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author | Mylkie, Kinga Nowak, Pawel Rybczynski, Patryk Ziegler-Borowska, Marta |
author_facet | Mylkie, Kinga Nowak, Pawel Rybczynski, Patryk Ziegler-Borowska, Marta |
author_sort | Mylkie, Kinga |
collection | PubMed |
description | Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials depends on their size, shape, core, and shell nature. Some of the most commonly used MNPs are those based on a magnetite core. On the other hand, synthetic biopolymers are used as a protective surface coating for these nanoparticles. This review describes the advances in the field of polymer-coated MNPs for protein immobilization over the past decade. General methods of MNP preparation and protein immobilization are presented. The most extensive section of this article discusses the latest work on the use of polymer-coated MNPs for the physical and chemical immobilization of three types of proteins: enzymes, antibodies, and serum proteins. Where possible, the effectiveness of the immobilization and the activity and use of the immobilized protein are reported. Finally, the information available in the peer-reviewed literature and the application perspectives for the MNP-immobilized protein systems are summarized as well. |
format | Online Article Text |
id | pubmed-7825442 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78254422021-01-24 Polymer-Coated Magnetite Nanoparticles for Protein Immobilization Mylkie, Kinga Nowak, Pawel Rybczynski, Patryk Ziegler-Borowska, Marta Materials (Basel) Review Since their discovery, magnetic nanoparticles (MNPs) have become materials with great potential, especially considering the applications of biomedical sciences. A series of works on the preparation, characterization, and application of MNPs has shown that the biological activity of such materials depends on their size, shape, core, and shell nature. Some of the most commonly used MNPs are those based on a magnetite core. On the other hand, synthetic biopolymers are used as a protective surface coating for these nanoparticles. This review describes the advances in the field of polymer-coated MNPs for protein immobilization over the past decade. General methods of MNP preparation and protein immobilization are presented. The most extensive section of this article discusses the latest work on the use of polymer-coated MNPs for the physical and chemical immobilization of three types of proteins: enzymes, antibodies, and serum proteins. Where possible, the effectiveness of the immobilization and the activity and use of the immobilized protein are reported. Finally, the information available in the peer-reviewed literature and the application perspectives for the MNP-immobilized protein systems are summarized as well. MDPI 2021-01-06 /pmc/articles/PMC7825442/ /pubmed/33419055 http://dx.doi.org/10.3390/ma14020248 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Mylkie, Kinga Nowak, Pawel Rybczynski, Patryk Ziegler-Borowska, Marta Polymer-Coated Magnetite Nanoparticles for Protein Immobilization |
title | Polymer-Coated Magnetite Nanoparticles for Protein Immobilization |
title_full | Polymer-Coated Magnetite Nanoparticles for Protein Immobilization |
title_fullStr | Polymer-Coated Magnetite Nanoparticles for Protein Immobilization |
title_full_unstemmed | Polymer-Coated Magnetite Nanoparticles for Protein Immobilization |
title_short | Polymer-Coated Magnetite Nanoparticles for Protein Immobilization |
title_sort | polymer-coated magnetite nanoparticles for protein immobilization |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825442/ https://www.ncbi.nlm.nih.gov/pubmed/33419055 http://dx.doi.org/10.3390/ma14020248 |
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