Cargando…

In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy

Ex-vivo and in-vivo skin analysis has been extensively evaluated by confocal Raman spectroscopy (CRS). The off-line measurement with a CRS-suited skin-mounted device after Franz-cell incubations is the most popular choice. However, real-time monitoring of in-line measurement has clear advantages for...

Descripción completa

Detalles Bibliográficos
Autores principales: Krombholz, Richard, Liu, Yali, Lunter, Dominique Jasmin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825688/
https://www.ncbi.nlm.nih.gov/pubmed/33430237
http://dx.doi.org/10.3390/pharmaceutics13010067
_version_ 1783640365406879744
author Krombholz, Richard
Liu, Yali
Lunter, Dominique Jasmin
author_facet Krombholz, Richard
Liu, Yali
Lunter, Dominique Jasmin
author_sort Krombholz, Richard
collection PubMed
description Ex-vivo and in-vivo skin analysis has been extensively evaluated by confocal Raman spectroscopy (CRS). The off-line measurement with a CRS-suited skin-mounted device after Franz-cell incubations is the most popular choice. However, real-time monitoring of in-line measurement has clear advantages for obtaining dynamic and more timely results. In our study, a custom-built setup suitable for in-line measurements was implemented, which ensures constant skin incubation and in-situ skin detections. We aim to compare the differences between using in-line and off-line devices for monitoring skin drug penetrations. A well-assessed formulation gel with procaine-HCl as the active ingredient was used as reference. The PEG-23 lauryl ether was added to the formulation as a penetration enhancer to evaluate the enhancement effects of procaine on skin. After incubation times of 14, 20, and 24 h, skin penetration profiles were assessed. Comparable results between off-line and in-line measurements were obtained. Remarkable improvements in penetrated procaine amount and depth were observed. Based on the significant differences of their enhanced penetration amounts, fairly similar estimations were achieved from both methods. A slight difference of 14 h incubation between these two setups can still be found, which may be due to the different detection conditions and affected skin properties. Overall, in-line measurements could provide a more time- and labor-saving alternative for off-line measurements in ex-vivo study.
format Online
Article
Text
id pubmed-7825688
institution National Center for Biotechnology Information
language English
publishDate 2021
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-78256882021-01-24 In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy Krombholz, Richard Liu, Yali Lunter, Dominique Jasmin Pharmaceutics Article Ex-vivo and in-vivo skin analysis has been extensively evaluated by confocal Raman spectroscopy (CRS). The off-line measurement with a CRS-suited skin-mounted device after Franz-cell incubations is the most popular choice. However, real-time monitoring of in-line measurement has clear advantages for obtaining dynamic and more timely results. In our study, a custom-built setup suitable for in-line measurements was implemented, which ensures constant skin incubation and in-situ skin detections. We aim to compare the differences between using in-line and off-line devices for monitoring skin drug penetrations. A well-assessed formulation gel with procaine-HCl as the active ingredient was used as reference. The PEG-23 lauryl ether was added to the formulation as a penetration enhancer to evaluate the enhancement effects of procaine on skin. After incubation times of 14, 20, and 24 h, skin penetration profiles were assessed. Comparable results between off-line and in-line measurements were obtained. Remarkable improvements in penetrated procaine amount and depth were observed. Based on the significant differences of their enhanced penetration amounts, fairly similar estimations were achieved from both methods. A slight difference of 14 h incubation between these two setups can still be found, which may be due to the different detection conditions and affected skin properties. Overall, in-line measurements could provide a more time- and labor-saving alternative for off-line measurements in ex-vivo study. MDPI 2021-01-07 /pmc/articles/PMC7825688/ /pubmed/33430237 http://dx.doi.org/10.3390/pharmaceutics13010067 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Krombholz, Richard
Liu, Yali
Lunter, Dominique Jasmin
In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy
title In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy
title_full In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy
title_fullStr In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy
title_full_unstemmed In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy
title_short In-Line and Off-Line Monitoring of Skin Penetration Profiles Using Confocal Raman Spectroscopy
title_sort in-line and off-line monitoring of skin penetration profiles using confocal raman spectroscopy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825688/
https://www.ncbi.nlm.nih.gov/pubmed/33430237
http://dx.doi.org/10.3390/pharmaceutics13010067
work_keys_str_mv AT krombholzrichard inlineandofflinemonitoringofskinpenetrationprofilesusingconfocalramanspectroscopy
AT liuyali inlineandofflinemonitoringofskinpenetrationprofilesusingconfocalramanspectroscopy
AT lunterdominiquejasmin inlineandofflinemonitoringofskinpenetrationprofilesusingconfocalramanspectroscopy