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Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro

Background and objectives: There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and...

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Autores principales: Kim, Yeojung, Yun, Sangwon, Shin, Keun-A, Chung, Woosuk, Ko, Youngkwon, Kim, Yoon-Hee, Lim, Chaeseong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825752/
https://www.ncbi.nlm.nih.gov/pubmed/33430347
http://dx.doi.org/10.3390/medicina57010045
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author Kim, Yeojung
Yun, Sangwon
Shin, Keun-A
Chung, Woosuk
Ko, Youngkwon
Kim, Yoon-Hee
Lim, Chaeseong
author_facet Kim, Yeojung
Yun, Sangwon
Shin, Keun-A
Chung, Woosuk
Ko, Youngkwon
Kim, Yoon-Hee
Lim, Chaeseong
author_sort Kim, Yeojung
collection PubMed
description Background and objectives: There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and outcomes of Lewis lung carcinoma (LLC) cells. Materials and Methods: A total of 37 mice were injected with LLC cells to compare the tumor size and survival of the sevoflurane exposed group (sevo group) and control group. The sevo group was exposed to 2% sevoflurane and 4 L/min of oxygen for 1 h per day 3 times per week, and the control group was exposed only to 4 L/min of oxygen. In vitro study, 12 plates incubated with LCC cells. 6 plates were exposed to 2% sevoflurane for 1 hr/day for 3 days and 6 plates were not exposed, and cell proliferation was compared after 3 days. Results: There were no significant differences in survival or tumor size between mice exposed to sevoflurane and control mice (survival: 29.06 ± 4.45 vs. 28.76 ± 3.75, p = 0.836; tumor size: 0.75 (0.41–1.02) vs. 0.49 (0.11–0.79), p = 0.153). However, in vitro study, the proliferation of LLC cells exposed to sevoflurane increased by 9.2% compared to the control group (p = 0.018). Conclusions: Sevoflurane (2 vol%) exposure could promote proliferation of LLC cells in vitro environment, but may not affect proliferation of LLC cells in vivo environment. These results suggest that in vitro studies on the effects of anesthetics on cancer may differ from those of in vivo or clinical studies.
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spelling pubmed-78257522021-01-24 Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro Kim, Yeojung Yun, Sangwon Shin, Keun-A Chung, Woosuk Ko, Youngkwon Kim, Yoon-Hee Lim, Chaeseong Medicina (Kaunas) Article Background and objectives: There are several studies that sevoflurane could enhance proliferation of cancer cells, while others suggest no effect on clinical outcome. We conducted in vivo and in vitro experiments to investigate the effects of sevoflurane, a volatile anesthetic, on proliferation and outcomes of Lewis lung carcinoma (LLC) cells. Materials and Methods: A total of 37 mice were injected with LLC cells to compare the tumor size and survival of the sevoflurane exposed group (sevo group) and control group. The sevo group was exposed to 2% sevoflurane and 4 L/min of oxygen for 1 h per day 3 times per week, and the control group was exposed only to 4 L/min of oxygen. In vitro study, 12 plates incubated with LCC cells. 6 plates were exposed to 2% sevoflurane for 1 hr/day for 3 days and 6 plates were not exposed, and cell proliferation was compared after 3 days. Results: There were no significant differences in survival or tumor size between mice exposed to sevoflurane and control mice (survival: 29.06 ± 4.45 vs. 28.76 ± 3.75, p = 0.836; tumor size: 0.75 (0.41–1.02) vs. 0.49 (0.11–0.79), p = 0.153). However, in vitro study, the proliferation of LLC cells exposed to sevoflurane increased by 9.2% compared to the control group (p = 0.018). Conclusions: Sevoflurane (2 vol%) exposure could promote proliferation of LLC cells in vitro environment, but may not affect proliferation of LLC cells in vivo environment. These results suggest that in vitro studies on the effects of anesthetics on cancer may differ from those of in vivo or clinical studies. MDPI 2021-01-07 /pmc/articles/PMC7825752/ /pubmed/33430347 http://dx.doi.org/10.3390/medicina57010045 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Kim, Yeojung
Yun, Sangwon
Shin, Keun-A
Chung, Woosuk
Ko, Youngkwon
Kim, Yoon-Hee
Lim, Chaeseong
Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
title Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
title_full Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
title_fullStr Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
title_full_unstemmed Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
title_short Effects of Sevoflurane on Lewis Lung Carcinoma Cell Proliferation In Vivo and In Vitro
title_sort effects of sevoflurane on lewis lung carcinoma cell proliferation in vivo and in vitro
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7825752/
https://www.ncbi.nlm.nih.gov/pubmed/33430347
http://dx.doi.org/10.3390/medicina57010045
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