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Precise and broad scope genome editing based on high-specificity Cas9 nickases

RNA-guided nucleases (RGNs) based on CRISPR systems permit installing short and large edits within eukaryotic genomes. However, precise genome editing is often hindered due to nuclease off-target activities and the multiple-copy character of the vast majority of chromosomal sequences. Dual nicking R...

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Detalles Bibliográficos
Autores principales:	Wang, Qian, Liu, Jin, Janssen, Josephine M, Le Bouteiller, Marie, Frock, Richard L, Gonçalves, Manuel A F V
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Oxford University Press 2021
Materias:	Synthetic Biology and Bioengineering
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826261/ https://www.ncbi.nlm.nih.gov/pubmed/33398349 http://dx.doi.org/10.1093/nar/gkaa1236

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