Droplet-based mRNA sequencing of fixed and permeabilized cells by CLInt-seq allows for antigen-specific TCR cloning

T cell receptors (TCRs) are generated by somatic recombination of V/D/J segments to produce up to 10(15) unique sequences. Highly sensitive and specific techniques are required to isolate and identify the rare TCR sequences that respond to antigens of interest. Here, we describe the use of mRNA sequ...

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Detalles Bibliográficos
Autores principales: Nesterenko, Pavlo A., McLaughlin, Jami, Cheng, Donghui, Bangayan, Nathanael J., Burton Sojo, Giselle, Seet, Christopher S., Qin, Yu, Mao, Zhiyuan, Obusan, Matthew B., Phillips, John W., Witte, Owen N.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: National Academy of Sciences 2021
Materias:
Biological Sciences
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826406/
https://www.ncbi.nlm.nih.gov/pubmed/33431692
http://dx.doi.org/10.1073/pnas.2021190118

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