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Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor

Bis(styryl) dye 1 bearing N-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe 1 selectively responds to the presence of Hg(2+) via the changes in the emission intensity as well as in...

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Autores principales: Panchenko, Pavel A., Efremenko, Anastasija V., Feofanov, Alexey V., Ustimova, Mariya A., Fedorov, Yuri V., Fedorova, Olga A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2021
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826577/
https://www.ncbi.nlm.nih.gov/pubmed/33440801
http://dx.doi.org/10.3390/s21020470
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author Panchenko, Pavel A.
Efremenko, Anastasija V.
Feofanov, Alexey V.
Ustimova, Mariya A.
Fedorov, Yuri V.
Fedorova, Olga A.
author_facet Panchenko, Pavel A.
Efremenko, Anastasija V.
Feofanov, Alexey V.
Ustimova, Mariya A.
Fedorov, Yuri V.
Fedorova, Olga A.
author_sort Panchenko, Pavel A.
collection PubMed
description Bis(styryl) dye 1 bearing N-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe 1 selectively responds to the presence of Hg(2+) via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 ± 0.15 µM). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500–600 nm) to red (600–730 nm) fluorescence intensity ratio decreased from 4.4 ± 0.2 to 1.43 ± 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg(2+) concentration in the 37 nM–1 μM range.
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spelling pubmed-78265772021-01-25 Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor Panchenko, Pavel A. Efremenko, Anastasija V. Feofanov, Alexey V. Ustimova, Mariya A. Fedorov, Yuri V. Fedorova, Olga A. Sensors (Basel) Article Bis(styryl) dye 1 bearing N-phenylazadithia-15-crown-5 ether receptor has been evaluated as a ratiometric fluorescent chemosensor for mercury (II) ions in living cells. In aqueous solution, probe 1 selectively responds to the presence of Hg(2+) via the changes in the emission intensity as well as in the emission band shape, which is a result of formation of the complex with 1:1 metal to ligand ratio (dissociation constant 0.56 ± 0.15 µM). The sensing mechanism is based on the interplay between the RET (resonance energy transfer) and ICT (intramolecular charge transfer) interactions occurring upon the UV/Vis (380 or 405 nm) photoexcitation of both styryl chromophores in probe 1. Bio-imaging studies revealed that the yellow (500–600 nm) to red (600–730 nm) fluorescence intensity ratio decreased from 4.4 ± 0.2 to 1.43 ± 0.10 when cells were exposed to increasing concentration of mercury (II) ions enabling ratiometric quantification of intracellular Hg(2+) concentration in the 37 nM–1 μM range. MDPI 2021-01-11 /pmc/articles/PMC7826577/ /pubmed/33440801 http://dx.doi.org/10.3390/s21020470 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Panchenko, Pavel A.
Efremenko, Anastasija V.
Feofanov, Alexey V.
Ustimova, Mariya A.
Fedorov, Yuri V.
Fedorova, Olga A.
Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
title Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
title_full Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
title_fullStr Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
title_full_unstemmed Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
title_short Ratiometric Detection of Mercury (II) Ions in Living Cells Using Fluorescent Probe Based on Bis(styryl) Dye and Azadithia-15-Crown-5 Ether Receptor
title_sort ratiometric detection of mercury (ii) ions in living cells using fluorescent probe based on bis(styryl) dye and azadithia-15-crown-5 ether receptor
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826577/
https://www.ncbi.nlm.nih.gov/pubmed/33440801
http://dx.doi.org/10.3390/s21020470
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