Cargando…
The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps
Recently, several studies explored associations between type 1 diabetes (T1DM) and microbiota. The aim of our study was to assess the colonic microbiota structure according to the metabolic control in T1DM patients treated with insulin pumps. We studied 89 T1DM patients (50.6% women) at the median a...
Autores principales: | , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826603/ https://www.ncbi.nlm.nih.gov/pubmed/33445500 http://dx.doi.org/10.3390/microorganisms9010155 |
_version_ | 1783640559925067776 |
---|---|
author | Mrozinska, Sandra Kapusta, Przemysław Gosiewski, Tomasz Sroka-Oleksiak, Agnieszka Ludwig-Słomczyńska, Agnieszka H. Matejko, Bartłomiej Kiec-Wilk, Beata Bulanda, Malgorzata Malecki, Maciej T. Wolkow, Pawel P. Klupa, Tomasz |
author_facet | Mrozinska, Sandra Kapusta, Przemysław Gosiewski, Tomasz Sroka-Oleksiak, Agnieszka Ludwig-Słomczyńska, Agnieszka H. Matejko, Bartłomiej Kiec-Wilk, Beata Bulanda, Malgorzata Malecki, Maciej T. Wolkow, Pawel P. Klupa, Tomasz |
author_sort | Mrozinska, Sandra |
collection | PubMed |
description | Recently, several studies explored associations between type 1 diabetes (T1DM) and microbiota. The aim of our study was to assess the colonic microbiota structure according to the metabolic control in T1DM patients treated with insulin pumps. We studied 89 T1DM patients (50.6% women) at the median age of 25 (IQR, 22–29) years. Pielou’s evenness (p = 0.02), and Shannon’s (p = 0.04) and Simpson’s diversity indexes (p = 0.01), were higher in patients with glycosylated hemoglobin (HbA1c) ≥ 53 mmol/mol (7%). There were no differences in beta diversity between groups. A linear discriminant analysis effect size (LEfSe) algorithm showed that one family (Ruminococcaceae) was enriched in patients with HbA1c < 53 mmol/mol, whereas one family (Streptococcaceae) and four species (Ruminococcus torques, unclassified species of Lactococcus, Eubacteroim dolichum, and Coprobacillus cateniformis) were enriched in patients with HbA1c ≥ 53 mmol/mol. We found that at class level, the following pathways according to Kyoto Encyclopedia of Genes and Genomes were enriched in patients with HbA1c < 53 mmol/mol: bacterial motility proteins, secretion system, bacterial secretion system, ribosome biogenesis, translation proteins, and lipid biosynthesis, whereas in patients with HbA1c ≥ 53 mmol/mol, the galactose metabolism, oxidative phosphorylation, phosphotransferase system, fructose, and mannose metabolism were enriched. Observed differences in alpha diversity, metabolic pathways, and associations between bacteria and HbA1c in colonic flora need further investigation. |
format | Online Article Text |
id | pubmed-7826603 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78266032021-01-25 The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps Mrozinska, Sandra Kapusta, Przemysław Gosiewski, Tomasz Sroka-Oleksiak, Agnieszka Ludwig-Słomczyńska, Agnieszka H. Matejko, Bartłomiej Kiec-Wilk, Beata Bulanda, Malgorzata Malecki, Maciej T. Wolkow, Pawel P. Klupa, Tomasz Microorganisms Article Recently, several studies explored associations between type 1 diabetes (T1DM) and microbiota. The aim of our study was to assess the colonic microbiota structure according to the metabolic control in T1DM patients treated with insulin pumps. We studied 89 T1DM patients (50.6% women) at the median age of 25 (IQR, 22–29) years. Pielou’s evenness (p = 0.02), and Shannon’s (p = 0.04) and Simpson’s diversity indexes (p = 0.01), were higher in patients with glycosylated hemoglobin (HbA1c) ≥ 53 mmol/mol (7%). There were no differences in beta diversity between groups. A linear discriminant analysis effect size (LEfSe) algorithm showed that one family (Ruminococcaceae) was enriched in patients with HbA1c < 53 mmol/mol, whereas one family (Streptococcaceae) and four species (Ruminococcus torques, unclassified species of Lactococcus, Eubacteroim dolichum, and Coprobacillus cateniformis) were enriched in patients with HbA1c ≥ 53 mmol/mol. We found that at class level, the following pathways according to Kyoto Encyclopedia of Genes and Genomes were enriched in patients with HbA1c < 53 mmol/mol: bacterial motility proteins, secretion system, bacterial secretion system, ribosome biogenesis, translation proteins, and lipid biosynthesis, whereas in patients with HbA1c ≥ 53 mmol/mol, the galactose metabolism, oxidative phosphorylation, phosphotransferase system, fructose, and mannose metabolism were enriched. Observed differences in alpha diversity, metabolic pathways, and associations between bacteria and HbA1c in colonic flora need further investigation. MDPI 2021-01-12 /pmc/articles/PMC7826603/ /pubmed/33445500 http://dx.doi.org/10.3390/microorganisms9010155 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Mrozinska, Sandra Kapusta, Przemysław Gosiewski, Tomasz Sroka-Oleksiak, Agnieszka Ludwig-Słomczyńska, Agnieszka H. Matejko, Bartłomiej Kiec-Wilk, Beata Bulanda, Malgorzata Malecki, Maciej T. Wolkow, Pawel P. Klupa, Tomasz The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps |
title | The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps |
title_full | The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps |
title_fullStr | The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps |
title_full_unstemmed | The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps |
title_short | The Gut Microbiota Profile According to Glycemic Control in Type 1 Diabetes Patients Treated with Personal Insulin Pumps |
title_sort | gut microbiota profile according to glycemic control in type 1 diabetes patients treated with personal insulin pumps |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826603/ https://www.ncbi.nlm.nih.gov/pubmed/33445500 http://dx.doi.org/10.3390/microorganisms9010155 |
work_keys_str_mv | AT mrozinskasandra thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT kapustaprzemysław thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT gosiewskitomasz thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT srokaoleksiakagnieszka thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT ludwigsłomczynskaagnieszkah thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT matejkobartłomiej thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT kiecwilkbeata thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT bulandamalgorzata thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT maleckimaciejt thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT wolkowpawelp thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT klupatomasz thegutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT mrozinskasandra gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT kapustaprzemysław gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT gosiewskitomasz gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT srokaoleksiakagnieszka gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT ludwigsłomczynskaagnieszkah gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT matejkobartłomiej gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT kiecwilkbeata gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT bulandamalgorzata gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT maleckimaciejt gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT wolkowpawelp gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps AT klupatomasz gutmicrobiotaprofileaccordingtoglycemiccontrolintype1diabetespatientstreatedwithpersonalinsulinpumps |