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RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control
RNA modifications are essential for proper RNA processing, quality control, and maturation steps. In the last decade, some eukaryotic DNA repair enzymes have been shown to have an ability to recognize and process modified RNA substrates and thereby contribute to RNA surveillance. Single-strand-selec...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826747/ https://www.ncbi.nlm.nih.gov/pubmed/33430019 http://dx.doi.org/10.3390/biom11010076 |
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author | Lirussi, Lisa Demir, Özlem You, Panpan Sarno, Antonio Amaro, Rommie E. Nilsen, Hilde |
author_facet | Lirussi, Lisa Demir, Özlem You, Panpan Sarno, Antonio Amaro, Rommie E. Nilsen, Hilde |
author_sort | Lirussi, Lisa |
collection | PubMed |
description | RNA modifications are essential for proper RNA processing, quality control, and maturation steps. In the last decade, some eukaryotic DNA repair enzymes have been shown to have an ability to recognize and process modified RNA substrates and thereby contribute to RNA surveillance. Single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1) is a base excision repair enzyme that not only recognizes and removes uracil and oxidized pyrimidines from DNA but is also able to process modified RNA substrates. SMUG1 interacts with the pseudouridine synthase dyskerin (DKC1), an enzyme essential for the correct assembly of small nucleolar ribonucleoproteins (snRNPs) and ribosomal RNA (rRNA) processing. Here, we review rRNA modifications and RNA quality control mechanisms in general and discuss the specific function of SMUG1 in rRNA metabolism. Cells lacking SMUG1 have elevated levels of immature rRNA molecules and accumulation of 5-hydroxymethyluridine (5hmU) in mature rRNA. SMUG1 may be required for post-transcriptional regulation and quality control of rRNAs, partly by regulating rRNA and stability. |
format | Online Article Text |
id | pubmed-7826747 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78267472021-01-25 RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control Lirussi, Lisa Demir, Özlem You, Panpan Sarno, Antonio Amaro, Rommie E. Nilsen, Hilde Biomolecules Review RNA modifications are essential for proper RNA processing, quality control, and maturation steps. In the last decade, some eukaryotic DNA repair enzymes have been shown to have an ability to recognize and process modified RNA substrates and thereby contribute to RNA surveillance. Single-strand-selective monofunctional uracil-DNA glycosylase 1 (SMUG1) is a base excision repair enzyme that not only recognizes and removes uracil and oxidized pyrimidines from DNA but is also able to process modified RNA substrates. SMUG1 interacts with the pseudouridine synthase dyskerin (DKC1), an enzyme essential for the correct assembly of small nucleolar ribonucleoproteins (snRNPs) and ribosomal RNA (rRNA) processing. Here, we review rRNA modifications and RNA quality control mechanisms in general and discuss the specific function of SMUG1 in rRNA metabolism. Cells lacking SMUG1 have elevated levels of immature rRNA molecules and accumulation of 5-hydroxymethyluridine (5hmU) in mature rRNA. SMUG1 may be required for post-transcriptional regulation and quality control of rRNAs, partly by regulating rRNA and stability. MDPI 2021-01-08 /pmc/articles/PMC7826747/ /pubmed/33430019 http://dx.doi.org/10.3390/biom11010076 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Lirussi, Lisa Demir, Özlem You, Panpan Sarno, Antonio Amaro, Rommie E. Nilsen, Hilde RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control |
title | RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control |
title_full | RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control |
title_fullStr | RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control |
title_full_unstemmed | RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control |
title_short | RNA Metabolism Guided by RNA Modifications: The Role of SMUG1 in rRNA Quality Control |
title_sort | rna metabolism guided by rna modifications: the role of smug1 in rrna quality control |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826747/ https://www.ncbi.nlm.nih.gov/pubmed/33430019 http://dx.doi.org/10.3390/biom11010076 |
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