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Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis
In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and o...
Autores principales: | , , , , , , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2021
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826819/ https://www.ncbi.nlm.nih.gov/pubmed/33440900 http://dx.doi.org/10.3390/microorganisms9010146 |
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author | Öhrman, Caroline Sahl, Jason W. Sjödin, Andreas Uneklint, Ingrid Ballard, Rebecca Karlsson, Linda McDonough, Ryelan F. Sundell, David Soria, Kathleen Bäckman, Stina Chase, Kitty Brindefalk, Björn Sozhamannan, Shanmuga Vallesi, Adriana Hägglund, Emil Ramirez-Paredes, Jose Gustavo Thelaus, Johanna Colquhoun, Duncan Myrtennäs, Kerstin Birdsell, Dawn Johansson, Anders Wagner, David M. Forsman, Mats |
author_facet | Öhrman, Caroline Sahl, Jason W. Sjödin, Andreas Uneklint, Ingrid Ballard, Rebecca Karlsson, Linda McDonough, Ryelan F. Sundell, David Soria, Kathleen Bäckman, Stina Chase, Kitty Brindefalk, Björn Sozhamannan, Shanmuga Vallesi, Adriana Hägglund, Emil Ramirez-Paredes, Jose Gustavo Thelaus, Johanna Colquhoun, Duncan Myrtennäs, Kerstin Birdsell, Dawn Johansson, Anders Wagner, David M. Forsman, Mats |
author_sort | Öhrman, Caroline |
collection | PubMed |
description | In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion in the scientific community. Historically, detection of Francisella tularensis in environmental samples has been challenging due to the considerable and unknown genetic diversity within the family, which can result in false positive results. We have assembled a comprehensive collection of genome sequences representing most known Francisellaceae species/strains and restructured them according to a taxonomy that is based on phylogenetic structure. From this structured dataset, we identified a small number of genomic regions unique to F. tularensis that are putatively suitable for specific detection of this pathogen in environmental samples. We designed and validated specific PCR assays based on these genetic regions that can be used for the detection of F. tularensis in environmental samples, such as water and air filters. |
format | Online Article Text |
id | pubmed-7826819 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2021 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-78268192021-01-25 Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis Öhrman, Caroline Sahl, Jason W. Sjödin, Andreas Uneklint, Ingrid Ballard, Rebecca Karlsson, Linda McDonough, Ryelan F. Sundell, David Soria, Kathleen Bäckman, Stina Chase, Kitty Brindefalk, Björn Sozhamannan, Shanmuga Vallesi, Adriana Hägglund, Emil Ramirez-Paredes, Jose Gustavo Thelaus, Johanna Colquhoun, Duncan Myrtennäs, Kerstin Birdsell, Dawn Johansson, Anders Wagner, David M. Forsman, Mats Microorganisms Article In recent years, an increasing diversity of species has been recognized within the family Francisellaceae. Unfortunately, novel isolates are sometimes misnamed in initial publications or multiple sources propose different nomenclature for genetically highly similar isolates. Thus, unstructured and occasionally incorrect information can lead to confusion in the scientific community. Historically, detection of Francisella tularensis in environmental samples has been challenging due to the considerable and unknown genetic diversity within the family, which can result in false positive results. We have assembled a comprehensive collection of genome sequences representing most known Francisellaceae species/strains and restructured them according to a taxonomy that is based on phylogenetic structure. From this structured dataset, we identified a small number of genomic regions unique to F. tularensis that are putatively suitable for specific detection of this pathogen in environmental samples. We designed and validated specific PCR assays based on these genetic regions that can be used for the detection of F. tularensis in environmental samples, such as water and air filters. MDPI 2021-01-11 /pmc/articles/PMC7826819/ /pubmed/33440900 http://dx.doi.org/10.3390/microorganisms9010146 Text en © 2021 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Öhrman, Caroline Sahl, Jason W. Sjödin, Andreas Uneklint, Ingrid Ballard, Rebecca Karlsson, Linda McDonough, Ryelan F. Sundell, David Soria, Kathleen Bäckman, Stina Chase, Kitty Brindefalk, Björn Sozhamannan, Shanmuga Vallesi, Adriana Hägglund, Emil Ramirez-Paredes, Jose Gustavo Thelaus, Johanna Colquhoun, Duncan Myrtennäs, Kerstin Birdsell, Dawn Johansson, Anders Wagner, David M. Forsman, Mats Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis |
title | Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis |
title_full | Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis |
title_fullStr | Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis |
title_full_unstemmed | Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis |
title_short | Reorganized Genomic Taxonomy of Francisellaceae Enables Design of Robust Environmental PCR Assays for Detection of Francisella tularensis |
title_sort | reorganized genomic taxonomy of francisellaceae enables design of robust environmental pcr assays for detection of francisella tularensis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7826819/ https://www.ncbi.nlm.nih.gov/pubmed/33440900 http://dx.doi.org/10.3390/microorganisms9010146 |
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